Abstract:
:The role of Shine-Dalgarno-like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far. In vitro translation analysis has revealed that the Shine-Dalgarno-like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in the protein-coding region of the rps14 mRNA. Editing of the second site was found to be partial and hence the partially edited transcripts are accumulated in tobacco green leaves. In vitro translation assays using the fully edited, partially edited and unedited rps14 mRNAs indicated that editing does not directly influence translational efficiency.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hirose T,Kusumegi T,Sugiura Mdoi
10.1016/s0014-5793(98)00673-5subject
Has Abstractpub_date
1998-07-03 00:00:00pages
257-60issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00673-5journal_volume
430pub_type
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