S-100 proteins and microtubules: analysis of the effects of rat brain S-100 (S-100b) and ox brain S-100a0, S-100a and S-100b on microtubule assembly-disassembly.

abstract：:It is 40 years since cancer growth was correlated with neovascularisation. Anti-angiogenic drugs remain at the forefront of cancer investigations but progress has been disappointing and unexpected toxicities are emerging. Gap junction channels are implicated in lesion spread following injury, with channel blockers sho...

journal_title：FEBS letters

authors： Zhang J,O'Carroll SJ,Henare K,Ching LM,Ormonde S,Nicholson LF,Danesh-Meyer HV,Green CR

更新日期：2014-04-17 00:00:00

abstract：:We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. T...

journal_title：FEBS letters

authors： Tsubuki S,Saito Y,Kawashima S

更新日期：1994-05-16 00:00:00

abstract：:A 32 kDa phospholipase A2 inhibitory protein was isolated from pig thyroid gland after calcium precipitation and fast protein liquid anion-exchange chromatography. SDS-polyacrylamide gel electrophoresis revealed the purity of the protein. The protein activity was assessed by the inhibition of pancreatic phospholipase ...

journal_title：FEBS letters

authors： Antonicelli F,Rothhut B,Martiny L,Aguie-Aguie G,Lambert B,Bellon G,Russo-Marie F,Jacquemin C,Haye B

更新日期：1988-08-01 00:00:00

abstract：:Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic different...

journal_title：FEBS letters

authors： Jia J,Tian Q,Ling S,Liu Y,Yang S,Shao Z

更新日期：2013-09-17 00:00:00

abstract：:Side chain-hydroxylated derivatives of cholesterol (OH sterol) inhibiting lymphoblastic transformation bind with high affinity and specificity to a hydroxysterol binding protein (OHSBP) in the cytosol of human lymphocytes. These binding properties of OHSBP suggested some analogies with that of steroid hormone receptor...

journal_title：FEBS letters

authors： Defay R,Astruc M,Beseme F,Descomps B,Crastes de Paulet A

更新日期：1984-08-06 00:00:00

abstract：:The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constan...

journal_title：FEBS letters

authors： Zenkova MA,Fedorova OS,Levina AS,Mamaev SV,Karpova GG

更新日期：1990-08-20 00:00:00

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

journal_title：FEBS letters

authors： Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

更新日期：2011-03-23 00:00:00

abstract：:The number of proteins found in body fluids and at cell surfaces, which are known to display carbohydrate-binding properties, continues to increase rapidly. In these proteins, in addition to a domain associated with lectin properties, one or more, non-lectin domains are present. It is possible that binding of sugar re...

journal_title：FEBS letters

authors： Thiel S,Reid KB

更新日期：1989-06-19 00:00:00

abstract：:Mutations in the presenilin (PS) genes PSI and PS2 are involved in Alzheimer's disease (AD). Recently, apoptosis-associated cleavage of PS proteins was identified. Here we demonstrate that PS1 as well as PS2 are substrates for different members of the caspase protein family. Remarkably, the caspases acting on PS1 coul...

journal_title：FEBS letters

authors： van de Craen M,de Jonghe C,van den Brande I,Declercq W,van Gassen G,van Criekinge W,Vanderhoeven I,Fiers W,van Broeckhoven C,Hendriks L,Vandenabeele P

更新日期：1999-02-19 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:Polyamine depletion, obtained in FAO cells with specific inhibitors of biosynthetic enzymes, prevents or decreases the accumulation of hsp 70 mRNA following heat shock [Desiderio et al., Hepatology 24 (1996) 150-156]. The present study shows that under conditions of spermidine depletion caused by alpha-difluoromethylo...

journal_title：FEBS letters

authors： Desiderio MA,Dansi P,Tacchini L,Bernelli-Zazzera A

更新日期：1999-07-16 00:00:00

abstract：:The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...

journal_title：FEBS letters

authors： Harricane MC,Cavadore C,Audemard E,Mornet D

更新日期：1990-08-20 00:00:00

abstract：:We report here the synthesis of novel 5'-linked oligodeoxynucleotides, both normal phosphodiester and phosphorothioate analogs, in which a covalently attached group at the 5'-terminus is an anthraquinone. These compounds represent a new class of antisense compounds in which the base sequence of the oligodeoxynucleotid...

journal_title：FEBS letters

authors： Mori K,Subasinghe C,Cohen JS

更新日期：1989-06-05 00:00:00

abstract：:ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA...

journal_title：FEBS letters

authors： Li Z,Liu D

更新日期：2012-05-07 00:00:00

abstract：:Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

journal_title：FEBS letters

authors： Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

更新日期：2003-01-02 00:00:00

abstract：:Extended X-ray absorption fine structure (EXAFS) spectra at the Cu K-edge have been recorded of the oxidized and reduced form at pH 3.5 of rusticyanin, the type-1 or 'blue'-copper protein from Thiobacillus ferrooxidans. The EXAFS of oxidized rusticyanin is well simulated with models assuming a ligand set of 2 N(His) a...

journal_title：FEBS letters

authors： Holt SD,Piggott B,Ingledew WJ,Feiters MC,Diakun GP

更新日期：1990-08-20 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level ...

journal_title：FEBS letters

authors： Stavri GT,Hong Y,Zachary IC,Breier G,Baskerville PA,Ylä-Herttuala S,Risau W,Martin JF,Erusalimsky JD

更新日期：1995-01-30 00:00:00

abstract：:The potent inhibitors of protein kinase C (PKC), H7, staurosporine, and staurosporine derivatives, were examined for their inhibitory effects on novel PKC (nPKC) isozymes delta and epsilon. H7 and staurosporine, usually used as selective inhibitors of PKC, showed similar inhibitory effects on cPKC (a mixture of cPKC a...

journal_title：FEBS letters

authors： Mizuno K,Saido TC,Ohno S,Tamaoki T,Suzuki K

更新日期：1993-09-13 00:00:00

abstract：:We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...

journal_title：FEBS letters

authors： Tabuchi Y,Takasaki I,Doi T,Ishii Y,Sakai H,Kondo T

更新日期：2006-05-29 00:00:00

abstract：:Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fraction...

journal_title：FEBS letters

authors： Hait NC,Fujita K,Lester RL,Dickson RC

更新日期：2002-12-04 00:00:00

abstract：:T cells express two different types of voltage-independent Ca(2+)-activated K(+) channels with small (SK) and intermediate (IK) conductance that serve important roles in the activation of T lymphocytes. In contrast to the IK channels from T lymphocytes which are upregulated upon mitogen stimulation, SK channels of Jur...

journal_title：FEBS letters

authors： Jäger H,Adelman JP,Grissmer S

更新日期：2000-03-10 00:00:00

abstract：:Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...

journal_title：FEBS letters

authors： Ohnuma T,Umemoto N,Kondo K,Numata T,Fukamizo T

更新日期：2013-08-19 00:00:00

abstract：:In cooperative aggregation of bovine platelets induced by simultaneous addition of PAF and collagen at subthreshold concentrations the following observations were made. (i) Formation of phosphatidic acid and arachidonic acid metabolites, which characterize PAF and collagen alone-induced aggregation, respectively, was ...

journal_title：FEBS letters

authors： Kojima S,Sekiya F,Inada Y,Sato F,Tsukada T,Saito Y

更新日期：1990-07-16 00:00:00

abstract：:In C4 plants the activity of phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is regulated by phosphorylation/dephosphorylation which is mediated by light/dark signals. The study using protein kinase inhibitors showed that the inhibition pattern of maize PEPC-protein kinase (PEPC-PK) is similar to that of myosin li...

journal_title：FEBS letters

authors： Ogawa N,Okumura S,Izui K

更新日期：1992-05-04 00:00:00

abstract：:The family of lipases (triacylglycerol-acyl-hydrolases EC 3.1.1.3) constitutes an interesting class of enzymes because of their ability to interact with lipid-water interfaces, their wide range of substrate specificities, and their potential industrial applications. Here we report the first crystal structure of a bact...

journal_title：FEBS letters

authors： Noble ME,Cleasby A,Johnson LN,Egmond MR,Frenken LG

更新日期：1993-09-27 00:00:00

abstract：:Abstract Hyperhomocysteinemia has recently been identified as an important risk factor for Alzheimer's disease (AD). One of the potential mechanisms underlying harmful effects of homocysteine (Hcy) is site-specific acylation of proteins at lysine residues by homocysteine thiolactone (HCTL). The accumulation of amyloid...

journal_title：FEBS letters

authors： Khodadadi S,Riazi GH,Ahmadian S,Hoveizi E,Karima O,Aryapour H

更新日期：2012-01-20 00:00:00

abstract：:The mediator complex in the RNA polymerase II holoenzyme is known to be involved in transcriptional activation. The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date. While the expression of Srb7p under the control of a repre...

journal_title：FEBS letters

authors： Gromöller A,Lehming N

更新日期：2000-10-27 00:00:00

abstract：:Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

journal_title：FEBS letters

authors： Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

更新日期：1999-07-09 00:00:00

abstract：:1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced c...

journal_title：FEBS letters

authors： Hamada M,Nishio K,Doe M,Usuki Y,Tanaka T

更新日期：2002-03-13 00:00:00