Abstract:
:Vascular endothelial growth factor (VEGF) mRNA expression was analysed in rabbit vascular smooth muscle cells following exposure to hypoxia and platelet-derived growth factor-BB (PDGF-BB). Hypoxia potently upregulated VEGF mRNA steady-state levels in a time- and concentration-dependent manner reaching a maximum level (approximately 30-fold increase) after 12-24 h at 0% 0(2). In contrast, PDGF-BB caused a modest increase in VEGF expression. However, the combination of PDGF-BB and a threshold hypoxic stimulus (2.5% O2 for 4 h) had a marked synergistic effect. Synergy between hypoxia and PDGF-BB was selective for VEGF expression as hypoxia had no effect on the PDGF-induced upregulation of the proto-oncogene c-myc. These results raise the possibility that hypoxia and PDGF-BB may act in concert to induce VEGF expression in the arterial wall during the development of atherosclerosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Stavri GT,Hong Y,Zachary IC,Breier G,Baskerville PA,Ylä-Herttuala S,Risau W,Martin JF,Erusalimsky JDdoi
10.1016/0014-5793(94)01458-dsubject
Has Abstractpub_date
1995-01-30 00:00:00pages
311-5issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)01458-Djournal_volume
358pub_type
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