Abstract:
:NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by wash-out in the presence of 1 mM L-arginine. In short-term incubations (45 s) of membrane fractions, L-NMA and L-NNA exhibited similar potencies to inhibit endothelial NO synthase, but L-NNA was markedly more potent than L-NMA after prolonged incubation periods (> or = 3 min) due to induction of a pronounced, reversible enzyme inactivation.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mayer B,Schmid M,Klatt P,Schmidt Kdoi
10.1016/0014-5793(93)80405-jsubject
Has Abstractpub_date
1993-10-25 00:00:00pages
203-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80405-Jjournal_volume
333pub_type
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