Abstract:
:Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the cloned enterohemolysin operon (hlyC, A, B, D genes) from an STEC human strain induced the production of interleukin-1beta (IL-1beta) through its mRNA expression but not tumor necrosis factor-alpha from human monocytes. No IL-1beta release was observed with an enterohemolysin (HlyA)-negative, isogenic E. coli strain carrying a mutation in the hlyA gene. The data suggest that enterohemolysin, a pore-forming toxin, induces the production of IL-1beta, which is one of serum risk markers for HUS.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Taneike I,Zhang HM,Wakisaka-Saito N,Yamamoto Tdoi
10.1016/s0014-5793(02)03027-2keywords:
subject
Has Abstractpub_date
2002-07-31 00:00:00pages
219-24issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302030272journal_volume
524pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Casein phosphopeptides beta-CN(1-25)4P and alpha(s1)-CN(59-79)5P, from beta- and alpha(s1)-casein, respectively, both carrying the characteristic 'acidic motif' Ser(P)-Ser(P)-Ser(P)-Glu-Glu, were chemically synthesized and administered to HT-29 cells differentiated in culture, which are a used model of intestinal epit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00741-5
更新日期:2003-09-11 00:00:00
abstract::A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80189-8
更新日期:1987-09-28 00:00:00
abstract::The characteristics of renal transport of L-alanine by luminal-membrane vesicles from proximal straight tubules (pars recta) of rabbit kidney were investigated. The following picture emerges from transport studies. Two electrogenic and Na+ requiring systems confined to this region of the nephron exist for the transpor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81408-x
更新日期:1988-01-18 00:00:00
abstract::The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01392-9
更新日期:2004-01-02 00:00:00
abstract::We provide first large scale analysis of the peculiarities of surface areas of 5658 dissimilar (below 50% sequence similarity) proteins with known 3D-structures that bind to proteins, DNA or RNAs. We show here that area of the protein surface is highly correlated with the protein length. The size of the interface surf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.014
更新日期:2015-09-14 00:00:00
abstract::There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.063
更新日期:2014-05-02 00:00:00
abstract::Flavonoid 3'-hydroxylase (F3'H) and flavonoid 3',5'-hydroxylase (F3'5'H) are cytochrome P450 enzymes and determine the B-ring hydroxylation pattern of flavonoids by introducing hydroxyl groups at the 3'- or the 3'- and 5'-position, respectively. Sequence identity between F3'H and F3'5'H is generally low since their di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.045
更新日期:2007-07-24 00:00:00
abstract::We have found that the sign of phototaxis, i.e. the direction of phototactic migration either towards or away from the light source, of wild-type Chlamydomonas reinhardtii depends on its photosynthetic activity. This accounts for the frequently reported transient positive phototaxis that precedes a negative phototaxis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80867-t
更新日期:1993-12-28 00:00:00
abstract::The class III phosphatidylinositol 3-kinase (PI3K-III) complex and its phosphorylated lipid product phosphatidylinositol 3-phosphate (PtdIns3P) control the three topologically related membrane-involution processes autophagy, endocytosis, and cytokinesis. The activity of the catalytic unit of PI3K-III complex, the Vacu...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.04.046
更新日期:2012-06-04 00:00:00
abstract::The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00565-q
更新日期:1995-06-26 00:00:00
abstract::The specific mechanism by which the inhibitory guanine nucleotide binding protein (Gi) mediates the inhibition of adenylate cyclase activity is still unclear. The subunit dissociation model, based on studies in purified or reconstituted systems, suggests that the beta gamma subunit, which is dissociated with activatio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80075-t
更新日期:1990-01-15 00:00:00
abstract::Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.07.036
更新日期:2013-09-17 00:00:00
abstract::A macrophage migration inhibitory factor (MIF), originally described as a product of activated lymphocytes, has been defined as a 12 kDa protein, expressed in a wide variety of tissues. Here MIF is identified as a phenylpyruvate tautomerase (EC 5.3.2.1) having p-hydroxyphenylpyruvate and phenylpyruvate as its natural ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01261-1
更新日期:1997-11-03 00:00:00
abstract::Enhancement by thrombin of Ca2+-dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80507-0
更新日期:1987-10-19 00:00:00
abstract::Proteases not only play a fundamental role in numerous physiological processes, but are also involved in several human diseases including Alzheimer's disease (AD). A key protease implicated in AD is the so far unidentified gamma-secretase, which cleaves the membrane-bound beta-amyloid precursor protein (betaAPP) at th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01627-0
更新日期:1999-12-17 00:00:00
abstract::We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS. We have tested mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01051-x
更新日期:1997-09-08 00:00:00
abstract::Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03475-0
更新日期:2002-10-23 00:00:00
abstract::Complementary (c) DNAs encoding a glycine receptor (GlyR) isomer were cloned from libraries constructed in lambda ZAPII with poly (A)+ RNA of neonatal rat spinal cord. Northern blot analysis revealed that RNA hybridized to the cloned cDNA is detectable only for a period of late embryonic/early postnatal stage of the s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80383-e
更新日期:1991-04-09 00:00:00
abstract::The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.002
更新日期:2009-10-06 00:00:00
abstract::Phenylalanine-specific tRNA from yeast was hydrolysed with cobra venom ribonuclease in the double-stranded regions and the fragments isolated. The 'dissected' molecules with nicks in positions 28 and 41 were reconstructed from supplementary fragments and treated with T-4 RNA ligase. A phosphodiester bond between two f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80021-6
更新日期:1984-01-02 00:00:00
abstract::Protein import into isolated tobacco mitochondria was investigated using mitochondria from leaves harvested at different times of the day and night. Efficient import was only detected with mitochondria isolated from leaves harvested during the dark period of the growth cycle, only low levels of import were detected fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01116-2
更新日期:1997-09-29 00:00:00
abstract::On the basis of the intron/exon organization and the intramolecular homology of DNA sequences, I propose a novel model for genesis of the calmodulin gene. A primordial calmodulin gene consisting of 51 base pairs (17 amino acids) was subjected to three-fold duplication to create modern calmodulin with four calcium-bind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80661-0
更新日期:1987-06-15 00:00:00
abstract::To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00302-x
更新日期:1996-04-15 00:00:00
abstract::We report here the synthesis of novel 5'-linked oligodeoxynucleotides, both normal phosphodiester and phosphorothioate analogs, in which a covalently attached group at the 5'-terminus is an anthraquinone. These compounds represent a new class of antisense compounds in which the base sequence of the oligodeoxynucleotid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80626-x
更新日期:1989-06-05 00:00:00
abstract::Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13674
更新日期:2020-03-01 00:00:00
abstract::Hterologous complementation of yeast mutants has enabled the isolation of genes encoding several families of amino acid transporters. Among them, NTR1 codes for a membrane protein with weak histidine transport activity. However, at the sequence level, NTR1 is related to rather non-specific oligopeptide transporters fr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00853-2
更新日期:1995-08-21 00:00:00
abstract::The H3K9 protein lysine methyltransferase SUV39H2 was reported to methylate K134 of H2AX and stimulate H2AX phosphorylation during DNA damage response [Sone K et al. (2014) Nat Commun 5, 5691]. However, the sequence context of H2AX-K134 differs from the specificity of SUV39H2. We performed in vitro methylation reactio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12216
更新日期:2016-06-01 00:00:00
abstract::A stabilization of both the precursor and the mature beta 1 integrin subunit was observed in metabolically labeled human skin fibroblasts and Molt-4 T lymphocytes upon addition of protein synthesis inhibitors or by ATP depletion. Differential effects of protein synthesis inhibitors are reported since the slow degradat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80171-p
更新日期:1993-07-26 00:00:00
abstract::The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03095-8
更新日期:2002-08-14 00:00:00
abstract::Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.10.051
更新日期:2008-12-10 00:00:00