Abstract:
:The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to detect independent rotation of the subunits in the native enzyme. The observed rotational correlation time (phi = 65 ns) corresponds to the rotation of a rather rigid dimeric structure. The microenvironment surrounding the natural probe pyridoxal-5-P covalently bound to the dimeric structure was explored using 31P-NMR at 72.86 MHz. In the native enzyme, the pyridoxal-5-P 31P-chemical shift is pH-independent, indicating that the phosphate group is well protected from the solvent. The correlation time determined from the 31P-spectrum of the aminotransferase exceeds the value calculated for the hydrated spherical model (phi = 40 ns). It is concluded that the phosphate of the pyridoxal-5-P molecule is rigidly bound to the active site of 4-aminobutyrate aminotransferase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Churchich JE,Kim DS,Schnackerz KDdoi
10.1016/0014-5793(83)80971-5subject
Has Abstractpub_date
1983-08-22 00:00:00pages
221-5issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(83)80971-5journal_volume
160pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Nucleoside analogues previously found to be inactive against the human immunodeficiency virus (HIV) may be activated by simple chemical derivatisation. As part of our effort to deliver masked phosphates inside living cells we have discovered that certain phosphate triester derivatives of inactive nucleoside analogues ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81580-s
更新日期:1993-05-17 00:00:00
abstract::Soluble factors derived from human CD8+ T-lymphocytes inhibit HIV-1 replication by suppressing transcription from the viral long terminal repeat (LTR), an effect shown to be mediated in part by an NFAT-1 enhancer sequence. We show here that the CD8+ derived beta-chemokines, RANTES, MIP1-alpha, and MIP-1beta, known sup...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00654-6
更新日期:1997-06-30 00:00:00
abstract::gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81142-1
更新日期:1989-09-25 00:00:00
abstract::Parathyroid hormone (PTH) binds to its receptor (PTH 1 receptor, PTH1R) and activates multiple pathways. The PTH1R, a class b GPCR, contains consensus calmodulin-binding motifs. The PTH1R cytoplasmic tail interacts with calmodulin in a calcium-dependent manner via the basic 1-5-8-14 motif. Calcium-dependent calmodulin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.12.056
更新日期:2005-01-31 00:00:00
abstract::A serum-free medium conditioned by U937, a human cell line of monocyte/macrophage origin, was found capable of inducing apoptosis on exponentially growing U937 cells themselves (autocrine suicide). The apoptosis-inducing agent is a macromolecule and possibly a protein (SKT factor), with a relative molecular mass in th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00287-8
更新日期:1994-05-09 00:00:00
abstract::The effects of the quaternary agent meproadifen on ACh-activated channel currents were studied on myoballs cultured from hind limb muscles of neonatal rats. Meproadifen (0.02-0.1 microM) combined with ACh (0.1-0.3 microM) in the patch pipette caused an increase, followed by a decrease, in the frequency of channel open...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81171-0
更新日期:1984-09-03 00:00:00
abstract::We report the identification of bovine miRNAs by cloning small RNAs from adipose tissue and the mammary gland. Fifty-nine distinct miRNAs were identified, five of them were not homologous to known mammalian miRNAs, and many of them had 3' and/or 5' end variants. Ribonuclease protection assays indicated that miR-23a an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.081
更新日期:2007-03-06 00:00:00
abstract::Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is common and is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core protein additively repress the universal cyclin-de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02694-7
更新日期:2002-05-08 00:00:00
abstract::Although signal transducer and activator of transcription 1 (STAT1) mediated regulation of p53 transcription and apoptosis has been previously reported, modulation of other members of the p53 family of transcription factors remains poorly understood. In this study, we found that STAT1 and TA-p73 can interact directly ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.049
更新日期:2007-03-20 00:00:00
abstract::Leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP) 2 signal via different receptor systems. We have recently demonstrated that simultaneous stimulation of fetal mouse neuroepithelial cells with these distinct types of cytokines synergistically induces astrocyte differentiation in a 2-day culture. He...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00997-7
更新日期:1999-08-20 00:00:00
abstract::The use of bisindolylmaleimide derivatives of staurosporine as selective inhibitors of protein kinase C (PKC) is in doubt following the report by Alessi [FEBS Lett. 402 (1997) 121-123] that Ro31-8220 and GF109203X are potent in vitro inhibitors of p70 S6 kinase and mitogen-activated protein kinase-activated protein ki...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01252-0
更新日期:1997-11-03 00:00:00
abstract::Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80661-x
更新日期:1985-05-06 00:00:00
abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03750-x
更新日期:2003-01-02 00:00:00
abstract::Tobacco SABP2, a 29kDa protein catalyzes the conversion of methyl salicylic acid (MeSA) into salicylic acid (SA) to induce SAR. Pretreatment of plants with acibenzolar-S-methyl (ASM), a functional analog of salicylic acid induces systemic acquired resistance (SAR). Data presented in this paper suggest that SABP2 catal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.046
更新日期:2010-08-04 00:00:00
abstract::Mitochondrial dihydroorotate dehydrogenase (DHOdehase; EC 1.3.99.11) is a target of anti-proliferative, immunosuppressive and anti-parasitic agents. Here, redoxal, (2,2'-[3,3'-dimethoxy[1, 1'-biphenyl]-4,4'-diyl)diimino]bis-benzoic acid, was studied with isolated mitochondria and the purified recombinant human and rat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01117-0
更新日期:2000-02-04 00:00:00
abstract::Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.06.077
更新日期:2006-07-24 00:00:00
abstract::The relationship between the antioxidant effects of reduced coenzyme Q10 (ubiquinol, UQH2) and vitamin E (alpha-tocopherol) was investigated in beef heart submitochondrial particles in which lipid peroxidation was initiated by incubation with ascorbate + ADP-Fe3+. These effects were examined after extraction of coenzy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80720-n
更新日期:1991-07-08 00:00:00
abstract::The 77 kDa subunit of the polyadenylation cleavage stimulation factor (CstF77) is important in messenger RNA 3' end processing. Previously, we demonstrated that AtCstF77 interacts with AtCPSF30, the Arabidopsis ortholog of the 30 kDa subunit of the Cleavage and Polyadenylation Specificity Factor. In further dissecting...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.03.007
更新日期:2010-04-16 00:00:00
abstract::The isocitrate lyase-encoding gene AgICL1 from the filamentous hemiascomycete Ashbya gossypii was isolated by heterologous complementation of a Saccharomyces cerevisiae icl1d mutant. The open reading frame of 1680 bp encoded a protein of 560 amino acids with a calculated molecular weight of 62584. Disruption of the Ag...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00017-4
更新日期:1999-02-05 00:00:00
abstract::A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80034-r
更新日期:1993-03-15 00:00:00
abstract::A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' term...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.05.035
更新日期:2013-07-11 00:00:00
abstract::The trend of the electric field and the value of the electric field flux, sensed by the superoxide substrate in the proximity of the active site, were found to be constant in three highly homologous Cu,Zn superoxide dismutases from ox, pig and sheep, which display large differences in net protein charge and distributi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80681-7
更新日期:1989-06-19 00:00:00
abstract::We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12247
更新日期:2016-07-01 00:00:00
abstract::The crystal structure for cce_0566 (171 aa, 19.4 kDa), a DUF269 annotated protein from the diazotrophic cyanobacterium Cyanothece sp. ATCC 51142, was determined to 1.60Å resolution. Cce_0566 is a homodimer with each molecule composed of eight α-helices folded on one side of a three strand anti-parallel β-sheet. Hydrop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.037
更新日期:2012-02-17 00:00:00
abstract::Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80026-6
更新日期:1970-01-15 00:00:00
abstract::The bi-functional protein dimerization cofactor of HNF1 (DCoH)/pterin-4 alpha-carbinolamine dehydratase (PCD) is found in liver cell nuclei bound to the transcription factor hepatocyte nuclear factor 1 (HNF1) as well as in the cytoplasm acting as an enzyme involved in the phenylalanine hydroxylation system. Deficiency...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01325-u
更新日期:1995-01-02 00:00:00
abstract::The lactase-phlorizin hydrolase gene was assigned to chromosome 2 by analysis of Southern blots of DNA from a panel of human-rodent cell hybrids containing characteristic sets of human chromosomes The hybridization probe used was a recently isolated cDNA clone of the human lactase-phlorizin hydrolase gene. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80352-1
更新日期:1988-11-21 00:00:00
abstract::Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.113
更新日期:2005-02-14 00:00:00
abstract::As a member of the dynamin superfamily human guanylate-binding protein 1 (hGBP1) binds and hydrolyses GTP thereby undergoing structural changes which lead to self-assembly of the protein. Here, we employ the reactivity of hGBP1 with a cysteine reactive compound in order to monitor structural changes imposed by GTP bin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.05.027
更新日期:2009-06-18 00:00:00
abstract::In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.04.072
更新日期:2011-06-23 00:00:00