The use of carotenoids and oxonol VI as probes for membrane potential in proteoliposomes.

Abstract:

:Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

van Walraven HS,Krab K,Hagendoorn MJ,Kraayenhof R

doi

10.1016/0014-5793(85)80661-x

subject

Has Abstract

pub_date

1985-05-06 00:00:00

pages

96-9

issue

1

eissn

0014-5793

issn

1873-3468

pii

0014-5793(85)80661-X

journal_volume

184

pub_type

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