Abstract:
:The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constant of cooperative binding of the reagent and the oligonucleotide-effector with 16 S rRNA was determined. The accuracy of modification was shown to double in the presence of 50 microM effector at 5 microM concentration of the reagent.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zenkova MA,Fedorova OS,Levina AS,Mamaev SV,Karpova GGdoi
10.1016/0014-5793(90)81110-asubject
Has Abstractpub_date
1990-08-20 00:00:00pages
26-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(90)81110-Ajournal_volume
269pub_type
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