Abstract:
:Treatment of MCF-7 breast cancer cells with 50 nM okadaic acid triggers an apoptotic response which is accompanied by a 7-fold increase in the activity of a protein kinase with a relative molecular mass of 53 kDa. The activity of the kinase was stimulated by cell treatment with inhibitors of phosphoprotein phosphatase 1 and 2A, but not by stressing conditions. Okadaic acid-induced stimulation of the 53 kDa protein kinase was not abolished by coincubation of cells with cycloheximide. We conclude that stimulation of the 53 kDa protein kinase by inhibitors of phosphoprotein phosphatases involves pre-existing molecular components whose activity depends on the phosphorylation state of serine/threonine residues.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rossini GP,Pinna C,Viviani Rdoi
10.1016/s0014-5793(97)00659-5subject
Has Abstractpub_date
1997-06-30 00:00:00pages
347-50issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00659-5journal_volume
410pub_type
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