Abstract:
:Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a fluorescent microscope. All the green embryos were shown to carry the transgene by PCR analysis. Taking advantage of the detection of GFP expression can be done non-invasively, the selected embryos were demonstrated to be able to developed to term with 100% of accuracy of the selection.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ikawa M,Kominami K,Yoshimura Y,Tanaka K,Nishimune Y,Okabe Mdoi
10.1016/0014-5793(95)01162-8subject
Has Abstractpub_date
1995-11-13 00:00:00pages
125-8issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(95)01162-8journal_volume
375pub_type
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