The X-ray structure of a divergent cyclophilin from the nematode parasite Brugia malayi.

abstract：:In this study we analyzed the interaction of prion protein PrP(C) with components of glycosphingolipid-enriched microdomains in lymphoblastoid T cells. PrP(C) was distributed in small clusters on the plasma membrane, as revealed by immunoelectron microscopy. PrP(C) is present in microdomains, since it coimmunoprecipit...

journal_title：FEBS letters

authors： Mattei V,Garofalo T,Misasi R,Circella A,Manganelli V,Lucania G,Pavan A,Sorice M

更新日期：2004-02-27 00:00:00

abstract：:The conversion of the cellular prion protein into the beta-sheet-rich scrapie prion protein is thought to be the key step in the pathogenesis of prion diseases. To gain insight into this structural conversion, we analyzed the intrinsic structural propensity of the amino acid sequence of the murine prion C-terminal dom...

journal_title：FEBS letters

authors： Jamin N,Coïc YM,Landon C,Ovtracht L,Baleux F,Neumann JM,Sanson A

更新日期：2002-10-09 00:00:00

abstract：:Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized p...

journal_title：FEBS letters

authors： Zubin EM,Romanova EA,Volkov EM,Tashlitsky VN,Korshunova GA,Shabarova ZA,Oretskaya TS

更新日期：1999-07-30 00:00:00

abstract：:The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All mole...

journal_title：FEBS letters

authors： Dornmair K

更新日期：1988-08-01 00:00:00

abstract：:We report phosphorylated and ubiquitinated aggregates of TAR DNA binding protein of 43 kDa (TDP-43) in SH-SY5Y cells similar to those in brains of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U). Two candidate sequences for the nuclear localization signa...

journal_title：FEBS letters

authors： Nonaka T,Arai T,Buratti E,Baralle FE,Akiyama H,Hasegawa M

更新日期：2009-01-22 00:00:00

abstract：:The bipartite human interleukin-4 (IL-4) receptor was functionally expressed in murine pro-B cells and activated by human IL-4 to evoke intracellular signaling. Mutual association of signal transducing proteins within the receptor complex was then studied in dependence of ligand stimulation. Besides ligand-induced rec...

journal_title：FEBS letters

authors： Erhardt I,Lischke A,Sebald W,Friedrich K

更新日期：1998-11-13 00:00:00

abstract：:Sulfated glycoprotein 2 (SGP-2) mRNA progressively increased in the ventral prostate of the aging rat, reaching, at 24 months, 4-fold higher than at 3 months. Ornithine decarboxylase (ODC) mRNA peaked at 6 months (4-fold increase), and at 12 and 24 months was maintained at higher levels than at 3 months. ODC enzymatic...

journal_title：FEBS letters

authors： Bettuzzi S,Strocchi P,Marinelli M,Astancolle S,Davalli P,Corti A

更新日期：1994-07-18 00:00:00

abstract：:Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to t...

journal_title：FEBS letters

authors： Torres J,Padrós E

更新日期：1993-02-22 00:00:00

abstract：:Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...

journal_title：FEBS letters

authors： Borrello S,Galeotti T,Palombini G,Minotti G

更新日期：1986-12-15 00:00:00

abstract：:More than 40 years have passed since the original publication by Hayflick and Moorhead led to the concept of the 'Hayflick limit' of the maximum number of divisions which somatic cells undergo in vitro. This concept is still regarded as a fundamental characteristic of species longevity. Here we want to emphasize anoth...

journal_title：FEBS letters

authors： Yegorov YE,Zelenin AV

更新日期：2003-04-24 00:00:00

abstract：:Neopterin is synthesized by human monocyte-derived macrophages primarily upon stimulation with the cytokine interferon-gamma. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutr...

journal_title：FEBS letters

authors： Razumovitch JA,Semenkova GN,Fuchs D,Cherenkevich SN

更新日期：2003-08-14 00:00:00

abstract：:Mouse spermatozoa stimulated with epidermal growth factor (EGF) or zona pellucida (ZP) experienced phosphatidylinositol 4,5-bisphosphate hydrolysis, diacylglycerol (DAG) generation and acrosomal exocytosis. The agonists showed additive effects but the action of EGF is likely to be mediated by a distinct receptor becau...

journal_title：FEBS letters

authors： Murase T,Roldan ER

更新日期：1995-03-06 00:00:00

abstract：:Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the ...

journal_title：FEBS letters

authors： Popescu IR,Helleboid-Chapman A,Lucas A,Vandewalle B,Dumont J,Bouchaert E,Derudas B,Kerr-Conte J,Caron S,Pattou F,Staels B

更新日期：2010-07-02 00:00:00

abstract：:To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...

journal_title：FEBS letters

authors： Fujita-Yoshigaki J,Shirouzu M,Koide H,Nishimura S,Yokoyama S

更新日期：1991-12-09 00:00:00

abstract：:The peptidic siderophore pyoverdine is the primary iron uptake system of fluorescent pseudomonads, and a virulence factor in the opportunistic pathogen Pseudomonas aeruginosa. Pyoverdine biogenesis is a co-ordinate process requiring several precursor-generating enzymes and large nonribosomal peptide synthetases (NRPSs...

journal_title：FEBS letters

authors： Imperi F,Visca P

更新日期：2013-11-01 00:00:00

abstract：:The fibroblast growth factor receptor substrate (FRS)-2 protein family comprises FRS2alpha, a well-known central mediator for fibroblast growth factor signaling, and FRS2beta, whose endogenous expression pattern and function are not yet defined. Immunohistochemical analysis revealed that expression of FRS2beta was res...

journal_title：FEBS letters

authors： Minegishi Y,Iwanari H,Mochizuki Y,Horii T,Hoshino T,Kodama T,Hamakubo T,Gotoh N

更新日期：2009-02-18 00:00:00

abstract：:The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

journal_title：FEBS letters

authors： Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

更新日期：1991-11-04 00:00:00

abstract：:A simple technique for rapid isolation of mitochondrial DNA (mtDNA) from animal cells is described. The method is based on the selective alkaline denaturation procedure of Birnboim and Doly [(1979) Nucleic Acids Res. 7, 1513-1523] and avoids the use of CsCl gradient centrifugation. The yield of mtDNA is comparable to ...

journal_title：FEBS letters

authors： Palva TK,Palva ET

更新日期：1985-11-18 00:00:00

abstract：:We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to inter...

journal_title：FEBS letters

authors： Stiles AR,Qian X,Shears SB,Grabau EA

更新日期：2008-06-11 00:00:00

abstract：:SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in ...

journal_title：FEBS letters

authors： Law PY,Liu YM,Geng H,Kwan KH,Waye MM,Ho YY

更新日期：2006-06-26 00:00:00

abstract：:Jasmonic acid (JA) and its derivatives are well-characterized signaling molecules in plant defense and development, but the site of their localization within plant tissue is entirely unknown. To address the question whether applied JA accumulates extracellularly or intracellularly, leaves of tomato and barley were fed...

journal_title：FEBS letters

authors： Bücking H,Förster H,Stenzel I,Miersch O,Hause B

更新日期：2004-03-26 00:00:00

abstract：:We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...

journal_title：FEBS letters

authors： Kaido T,Yamaoka S,Seto S,Funaki N,Kasamatsu T,Tanaka J,Nakamura T,Imamura M

更新日期：1997-07-14 00:00:00

abstract：:Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine ...

journal_title：FEBS letters

authors： Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJ

更新日期：1994-09-12 00:00:00

abstract：:The hypothesis that calpain subunits dissociate in the presence of Ca2+ has been tested by methods which avoid interference by Ca2+-induced aggregation and large subunit autolysis. Inactive Cys105Ser-m-calpain, bound either to Ni-NTA-agarose or to immobilized casein, after incubation with Ca2+, could be recovered in h...

journal_title：FEBS letters

authors： Dutt P,Arthur JS,Croall DE,Elce JS

更新日期：1998-10-09 00:00:00

abstract：:Ca(2+)-dependent protein kinases (CDPKs) play an essential role in plant Ca(2+)-mediated signal transduction. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. Eight of them were reported previously to be inducible by different stress sti...

journal_title：FEBS letters

authors： Wan B,Lin Y,Mou T

更新日期：2007-03-20 00:00:00

abstract：:Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...

journal_title：FEBS letters

authors： Matsuura M,Kojima Y,Homma JY,Kubota Y,Yamamoto A,Kiso M,Hasegawa A

更新日期：1984-02-27 00:00:00

abstract：:Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase bindi...

journal_title：FEBS letters

authors： Sasaki T,Göhring W,Miosge N,Abrams WR,Rosenbloom J,Timpl R

更新日期：1999-10-29 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:The mutation of Asp198 to Asn in the receptor for glucagon-like peptide-1(7-36)amide (GLP-1) had no effect upon GLP-1 affinity whereas substitution with Ala greatly reduced affinity, demonstrating the importance of polarity rather than negative charge at Asp198. However, the Asp198-Ala mutation had less effect upon th...

journal_title：FEBS letters

authors： López de Maturana R,Donnelly D

更新日期：2002-10-23 00:00:00

abstract：:FITC-labeled LPS from Neisseria meningitidis can be used as a probe to follow the process of LPS incorporation into liposomal membrane and to study its interaction with a bilayer. The incorporation of FITC-LPS into the bilayer was proved by physicochemical methods as well as by liposomal LPS toxicity decrease in actin...

journal_title：FEBS letters

authors： Trubetskoy VS,Koshkina NV,Omel'yanenko VG,L'vov VL,Dmitriev BA,Petrov AB,Torchilin VP

更新日期：1990-08-20 00:00:00