Abstract:
:The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilise alpha-MSH receptor proteins from cultured primary rat Schwann cells. [125I-Tyr2,Nle4,D-Phe7,ATB-Lys11]-alpha-MSH photoaffinity labelled proteins from Schwann cells were analyzed by SDS-PAGE followed by autoradiography. The results indicate that the alpha-MSH receptor proteins labelled have a molecular weight of 42-45 kDa. These data are the first to demonstrate solubilisation and characterisation of alpha-MSH receptors from non-melanoma cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dyer JK,Ahmed AR,Oliver GW,Poulton CW,Haynes LWdoi
10.1016/0014-5793(93)81619-bsubject
Has Abstractpub_date
1993-12-20 00:00:00pages
103-6issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81619-Bjournal_volume
336pub_type
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