Physico-chemical properties of the hydroxysterol binding protein of human lymphocyte cytosol. Effects of high salt concentrations and molybdate.

abstract：:CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within...

journal_title：FEBS letters

authors： Sorice M,Garofalo T,Misasi R,Longo A,Mattei V,Sale P,Dolo V,Gradini R,Pavan A

更新日期：2001-09-28 00:00:00

abstract：:Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

journal_title：FEBS letters

authors： Suzuki T,Kovacs MS,Board PG

更新日期：1990-11-26 00:00:00

abstract：:The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titi...

journal_title：FEBS letters

authors： Jäckel M,Witt C,Antonova O,Curdt I,Labeit S,Jockusch H

更新日期：1997-05-12 00:00:00

abstract：:In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects ...

journal_title：FEBS letters

authors： Yoshida T,Klinkspoor JH,Kuver R,Wrenn SP,Kaler EW,Lee SP

更新日期：2000-07-28 00:00:00

abstract：:Sarcoplasmic reticulum vesicles were solubilized stepwise by the nonionic detergent octaethyleneglycol monododecyl ether; 31P NMR enabled the extent of phospholipid solubilization to be monitored by following the conversion of the broad resonance peak characterizing the phospholipids inserted in the bilayer to the nar...

journal_title：FEBS letters

authors： Roux M,Champeil P

更新日期：1984-06-11 00:00:00

abstract：:Agonist-induced changes in expression and mRNA levels of luteinizing hormone (LH) receptors were compared during stimulation of ovarian follicular maturation and luteinization by gonadotropic hormones. Three major species of LH receptor mRNA, 5.8, 2.6 and 2.3 kb, were present throughout differentiation and changed sim...

journal_title：FEBS letters

authors： Hu ZZ,Tsai-Morris CH,Buczko E,Dufau ML

更新日期：1990-11-12 00:00:00

abstract：:Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...

journal_title：FEBS letters

authors： van der Goot FG,Pugin J,Hribar M,Fransen L,Dunant Y,De Baetselier P,Bloc A,Lucas R

更新日期：1999-10-22 00:00:00

abstract：:1,3-alpha-D-Glucan synthase of Streptococcus mutans catalyzes both the hydrolysis of sucrose to glucose and fructose, and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan. The enzyme catalyzes only sucrose hydrolysis, however, in the absence of 1,6-alpha-D-glucan as an acceptor. In the presen...

journal_title：FEBS letters

authors： Yamashita Y,Hanada N,Itoh-Andoh M,Takehara T

更新日期：1989-01-30 00:00:00

abstract：:Glycogen metabolism in β-cells may affect downstream metabolic pathways controlling insulin release. We examined glycogen metabolism in human islets and in the rodent-derived INS-1 832/13 β-cells and found them to express the same isoforms of key enzymes required for glycogen metabolism. Our findings indicate that gly...

journal_title：FEBS letters

authors： Andersson LE,Nicholas LM,Filipsson K,Sun J,Medina A,Al-Majdoub M,Fex M,Mulder H,Spégel P

更新日期：2016-12-01 00:00:00

abstract：:The distribution of flux control coefficients of a glycolytic system in starved rat liver has been determined. The flux control coefficient profile in starved conditions is compared with normal fed conditions showing that in the former phosphofructokinase enhances more than 2-fold its flux control coefficient while gl...

journal_title：FEBS letters

authors： Torres NV,Mateo F,Meléndez-Hevia E

更新日期：1988-06-06 00:00:00

abstract：:Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...

journal_title：FEBS letters

authors： Park MH,Park SY,Kim Y

更新日期：2008-02-06 00:00:00

abstract：:Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the inhibitors N-tosyl-L-lysyl chloromethylketone (TLCK) and N-tosyl-L-phenylalanyl chloromethylketone (TPCK) have very different effects on apoptosis. TLCK inhibits apoptosis ...

journal_title：FEBS letters

authors： Fearnhead HO,Rivett AJ,Dinsdale D,Cohen GM

更新日期：1995-01-09 00:00:00

abstract：:The proteolytic processing of rabbit intestinal lactase-phlorizin-hydrolase (LPH) was studied by pulse-chase and continuous labeling experiments in organ culture from 15-day-old rabbits in the presence of glycosylation and processing inhibitors. Monensin and brefeldin A inhibited the two proteolytic cleavages of the p...

journal_title：FEBS letters

authors： Rossi M,Maiuri L,Salvati VM,Russomanno C,Auricchio S

更新日期：1993-12-27 00:00:00

abstract：:The primary structure of 20 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homolog...

journal_title：FEBS letters

authors： Marekov L,Krook M,Jörnvall H

更新日期：1990-06-18 00:00:00

abstract：:The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

journal_title：FEBS letters

authors： Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

更新日期：1984-01-23 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:Fibromodulin and lumican are closely related members of the extracellular matrix leucine-rich repeat glycoprotein/proteoglycan family. Similar to decorin, another member of this protein family, they bind to fibrillar collagens and function in the assembly of the collagen network in connective tissues. We have studied ...

journal_title：FEBS letters

authors： Svensson L,Närlid I,Oldberg A

更新日期：2000-03-24 00:00:00

abstract：:Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...

journal_title：FEBS letters

authors： Wang Y,Han R,Zhang W,Yuan Y,Zhang X,Long Y,Mi H

更新日期：2008-03-05 00:00:00

abstract：:Signaling via G-protein coupled receptors is initiated by receptor-catalyzed nucleotide exchange on Galpha subunits normally bound to GDP and Gbetagamma. Activated Galpha . GTP then regulates effectors such as adenylyl cyclase. Except for Gbetagamma, no known regulators bind the adenylyl cyclase-stimulatory subunit Ga...

journal_title：FEBS letters

authors： Johnston CA,Ramer JK,Blaesius R,Fredericks Z,Watts VJ,Siderovski DP

更新日期：2005-10-24 00:00:00

abstract：:Methyl-beta-cyclodextrin (MbetaCD) was used to explore a role for cholesterol-enriched plasma membrane microdomains in coupling lysophosphatidic acid (LPA) stimulation to phosphoinositide 3-kinase (PI3K) activation. Cholesterol depletion strongly inhibited the production of phosphatidylinositol 3,4-bisphosphate and ph...

journal_title：FEBS letters

authors： Peres C,Yart A,Perret B,Salles JP,Raynal P

更新日期：2003-01-16 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae the genes involved in galactose metabolism (GAL1,7,10) are transcriptionally activated more than a 1000-fold in the presence of galactose as the sole carbon source in the culture media. In the present work, we monitored the activity of the GAL10 gene promoter in different Ras-cAMP...

journal_title：FEBS letters

authors： Mirisola MG,Gallo A,De Leo G

更新日期：2007-05-15 00:00:00

abstract：:The up-regulation of HER2/neu is associated with human malignancies and is a useful target for developing anticancer drugs. Overexpression of human manganese superoxide dismutase (MnSOD) has been demonstrated to effectively suppress various carcinoma cells, including breast carcinomas, in vitro and in vivo. This study...

journal_title：FEBS letters

authors： Chuang TC,Liu JY,Lin CT,Tang YT,Yeh MH,Chang SC,Li JW,Kao MC

更新日期：2007-09-18 00:00:00

abstract：:This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...

journal_title：FEBS letters

authors： Han Q,Li J

更新日期：2002-09-11 00:00:00

abstract：:Dystrophin, a component of the muscle membrane cytoskeleton, is the protein altered in Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Dystrophin shares significant homology with other cytoskeletal proteins, such as alpha-actinin and spectrin. On the basis of its sequence similarity with alpha-a...

journal_title：FEBS letters

authors： Chan Y,Kunkel LM

更新日期：1997-06-30 00:00:00

abstract：:Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...

journal_title：FEBS letters

authors： Palombo I,Daley DO

更新日期：2012-11-30 00:00:00

abstract：:Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...

journal_title：FEBS letters

authors： Hrabák A,Bajor T,Temesi A,Mészáros G

更新日期：1996-07-22 00:00:00

abstract：:Pretreatment of tissues with potassium channel openers (KCO's) has been observed to be cytoprotective in a broad variety of insults. This phenomenon has been proposed to be intimately linked to activation of mitochondrial potassium channels which apparently modulate the mitochondrial production of reactive oxygen spec...

journal_title：FEBS letters

authors： Malinska D,Mirandola SR,Kunz WS

更新日期：2010-05-17 00:00:00

abstract：:The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken ...

journal_title：FEBS letters

authors： Chen YH,Chen MX,Alessi DR,Campbell DG,Shanahan C,Cohen P,Cohen PT

更新日期：1994-12-12 00:00:00

abstract：:Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfe...

journal_title：FEBS letters

authors： Camborde L,Tournier V,Noizet M,Jupin I

更新日期：2007-01-23 00:00:00

abstract：:A hybrid protein from Neisseria meningitidis, which contains both a peroxiredoxin and a glutaredoxin domain, has been isolated. The enzyme was active in the reduction of various peroxides and dehydroascorbate in the presence of reduced glutathione. These findings suggest that both the peroxiredoxin and glutaredoxin do...

journal_title：FEBS letters

authors： Rouhier N,Jacquot JP

更新日期：2003-11-06 00:00:00