Abstract:
:In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects in cultured human pulmonary artery endothelial cells (HPAE). Oxysterols inhibited cell proliferation in a dose-dependent fashion. Oxysterols inhibited cell growth to 50% of control at a higher dose for DGBE cells than for HPAE cells. TriolC was more cytotoxic than 7KC. We also investigated the effect of oxysterols on bile salt-induced mucin secretion by DGBE cells. TriolC suppressed mucin secretion by DGBE cells, whereas 7KC did not. These findings support the hypothesis that biliary oxysterols affect gallbladder mucosal function.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yoshida T,Klinkspoor JH,Kuver R,Wrenn SP,Kaler EW,Lee SPdoi
10.1016/s0014-5793(00)01831-7keywords:
subject
Has Abstractpub_date
2000-07-28 00:00:00pages
113-8issue
1-2eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01831-7journal_volume
478pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We provide first large scale analysis of the peculiarities of surface areas of 5658 dissimilar (below 50% sequence similarity) proteins with known 3D-structures that bind to proteins, DNA or RNAs. We show here that area of the protein surface is highly correlated with the protein length. The size of the interface surf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.014
更新日期:2015-09-14 00:00:00
abstract::Using time-resolved low-temperature spectroscopy, we have examined whether or not bovine rhodopsin has a unique transducin-binding state, meta Ib, previously detected from chicken rhodopsin. Unlike chicken meta Ib, bovine meta Ib was detected only by detailed kinetics analysis of the bleaching process, but it was stab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00216-6
更新日期:1998-03-20 00:00:00
abstract::We have previously identified an ESE in NF1 exon 37 whose disruption by the pathological mutation c.6792C>G caused aberrant splicing. We now investigate the RNA-protein complexes affected by the c.6792C>G mutation observing that this concurrently decreases the affinity for the positive splicing factor YB-1 and increas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.05.018
更新日期:2008-06-25 00:00:00
abstract::Maculatin 1.1 is an antimicrobial peptide isolated from the Australian tree frog Litoria genimaculata that adopts an amphipathic, alpha-helical structure in solution. Its orientation and conformation when incorporated to pre-formed DMPG (1,2-dimyristoyl-sn-glycero-3-phosphoglycerol) and DMPC (1,2-dimyristoyl-sn-glycer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03313-0
更新日期:2002-02-13 00:00:00
abstract::Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00820-0
更新日期:1999-07-09 00:00:00
abstract::The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase whether or not the regulatory subunit (p85 alpha) was present, sugge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80482-6
更新日期:1994-04-04 00:00:00
abstract::A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80942-9
更新日期:1983-08-22 00:00:00
abstract::Ribonuclease T1 can be unfolded and refolded without forming noticeable amounts of aggregates allowing to characterise the dimensions of a protein in different denatured states in terms of the Stokes radius RS. Upon thermal unfolding RS increases from 1.74 nm at 20 degrees C to 2.14 nm at 60 degrees C. By contrast, RS...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00058-6
更新日期:1997-02-24 00:00:00
abstract::DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00136-2
更新日期:1999-02-26 00:00:00
abstract::According to the endosymbiont hypothesis, mitochondria are descended from ancient aerobic bacteria that were engulfed by protoeukaryotic cells. Experiments described here show that a synthetic peptide corresponding to a yeast mitochondrial targeting sequence can be imported into Paracoccus denitrificans, a soil bacter...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80619-5
更新日期:1994-01-03 00:00:00
abstract::In thrombin-stimulated platelets alpha IIb beta 3 integrin engagement triggers both phosphatidylinositol 3',4'-bisphosphate synthesis and calpain activation. We checked the possible involvement of calpains in phosphatidylinositol 3-kinase signalling pathway using a cell permeant specific inhibitor of calpains, calpept...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00079-3
更新日期:1997-03-03 00:00:00
abstract::Agonist-induced changes in expression and mRNA levels of luteinizing hormone (LH) receptors were compared during stimulation of ovarian follicular maturation and luteinization by gonadotropic hormones. Three major species of LH receptor mRNA, 5.8, 2.6 and 2.3 kb, were present throughout differentiation and changed sim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81359-v
更新日期:1990-11-12 00:00:00
abstract::The adipose-derived plasma protein, adiponectin (APN), has various protective effects on cardiovascular diseases. In this study, we show that endogenous APN is required for full cyclooxygenase-2 (COX-2) induction by ischemia-reperfusion injury in the heart in vivo. In rat neonatal cardiac myocytes, APN-induced COX-2 e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.002
更新日期:2008-04-02 00:00:00
abstract::Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80942-n
更新日期:1993-08-16 00:00:00
abstract::This paper reports the genomic analysis of the strain CBS7064 of Pichia sorbitophila, a homothallic diploid yeast. We sequenced 4829 random sequence tags of about 1 kb and compared them to the Saccharomyces cerevisiae gene products. Approximately 1300 nuclear genes, 22 tRNAs, the rDNA locus, and six mitochondrial gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02286-9
更新日期:2000-12-22 00:00:00
abstract::Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local fluid shear stress. Because the Leu33Pro (Pl(A)) polymorphism of integrin beta(3) confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00170-4
更新日期:2003-04-10 00:00:00
abstract::Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to inves...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.068
更新日期:2006-01-23 00:00:00
abstract::Unlike mammalian lysosomal cysteine proteases, the trypanosomal cysteine protease cruzipain contains a 130-amino acid residue C-terminal domain, in addition to the catalytic domain, and it is stable at neutral pH. The endogenous (with C-terminal domain) and recombinant (without C-terminal domain) cruzipains exhibit si...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01221-7
更新日期:2000-03-03 00:00:00
abstract::Nuclear cap-binding protein (CBP) 80/20-dependent translation (CT) is one of the targets for miRNA-mediated gene silencing. Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is tightly coupled to CT. Teth...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.07.047
更新日期:2011-09-02 00:00:00
abstract::Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02422-x
更新日期:2001-05-11 00:00:00
abstract::The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.009
更新日期:2007-04-03 00:00:00
abstract::The gamma subunit of the F1 moiety of the bovine mitochondrial H(+)-ATP synthase is shown to function as a component of the gate. Addition of purified gamma subunit to F0-liposomes inhibits transmembrane proton conduction. This inhibition can be removed by the bifunctional thiol reagent diamide. Immunoblot analysis sh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80462-r
更新日期:1990-10-15 00:00:00
abstract::Plasminogen activator inhibitor-1 (PAI-1) increases injury in several liver, lung and kidney disease models. The objective of this investigation was to assess the effect of PAI-1 deficiency on cholestatic liver fibrosis and determine PAI-1 influenced fibrogenic mechanisms. We found that PAI-1(-/-) mice had less fibros...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.049
更新日期:2007-06-26 00:00:00
abstract::Autophagy is an evolutionarily conserved stress response that promotes the lysosomal degradation of intracellular components. The bone marrow stromal cell-derived growth inhibitor (BDGI) functions as a stress sensor which is upregulated by oxidative stress and DNA damage. However, the role of BDGI in autophagic respon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13732
更新日期:2020-04-01 00:00:00
abstract::Fourteen truncated forms of Leuconostoc mesenteroides NRRL B512-F dextransucrase, involving N-, C- or N- plus C-terminal domain truncations were tested for their ability to bind dextrans. The shortest fragment (14kDa molecular weight) that still exhibited a strong interaction with dextran was localized between amino a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.08.062
更新日期:2007-10-02 00:00:00
abstract::A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81130-2
更新日期:1983-06-27 00:00:00
abstract::Oncostatin M (OSM) is a potent modulator of human lung-derived epithelial cell function. This cytokine binds two distinct receptor complexes: type I OSM receptor which is also a functional receptor for leukemia inhibitory factor (LIF), and type II OSM-specific receptor. The role of these two distinct receptors in medi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00643-7
更新日期:1998-06-16 00:00:00
abstract::We have attempted to elucidate the mechanism of apoptotic cell death induced by hypoxia (very low oxygen conditions) in neuronal cells. Human neuroblastoma SK-N-MC cells under hypoxic conditions resulted in apoptosis in a time-dependent manner estimated by DNA fragmentation assay and nuclear morphology stained with fl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01363-5
更新日期:1998-11-13 00:00:00
abstract::Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(+)]) remains above ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.034
更新日期:2006-09-18 00:00:00
abstract::Chlorella virus-encoded membrane protein Kcv represents a new class of potassium channel. This 94-amino acids miniature K(+) channel consists of two trans-membrane alpha-helix domains intermediated by a pore domain that contains a highly conserved K(+) selectivity filter. Therefore, as an archetypal K(+) channel, the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.005
更新日期:2007-03-06 00:00:00