Abstract:
:Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local fluid shear stress. Because the Leu33Pro (Pl(A)) polymorphism of integrin beta(3) confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the Leu33 or Pro33 isoform of beta(3) in flow chamber experiments to test whether shear forces would alter the Pl(A) adhesive phenotype. We found that shear force augmented the Pro33-mediated enhanced adhesion to fibrinogen. This Pro33-dependent enhancement was aspirin-sensitive and was also observed on immobilized von Willebrand factor and cryoprecipitate, but not fibronectin. Thus, shear stress enhances the adhesive phenotype of the Pro33 cells to multiple physiologic substrates.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vijayan KV,Huang TC,Liu Y,Bernardo A,Dong JF,Goldschmidt-Clermont PJ,Alevriadou BR,Bray PFdoi
10.1016/s0014-5793(03)00170-4keywords:
subject
Has Abstractpub_date
2003-04-10 00:00:00pages
41-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303001704journal_volume
540pub_type
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