Abstract:
:The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact with a minor fraction of Pol I when rDNA transcription is active. Surprisingly, Rrn3p, another Pol I-specific initiation factor, is more abundant in cell lysates than UAF and CF. Our analyses revealed that a large fraction of cellular Rrn3p is not associated with Pol I. However, the amount of initiation-active Rrn3p which forms a stable complex with Pol I corresponds to the levels of UAF and CF which have been shown to bind the promoter. Initiation-active Rrn3p dissociates from the template during or immediately after Pol I has switched from initiation to elongation. Our data support a model in which the elongating Pol I leaves the initiation factors UAF, CF and Rrn3p close by the promoter.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bier M,Fath S,Tschochner Hdoi
10.1016/S0014-5793(04)00311-4keywords:
subject
Has Abstractpub_date
2004-04-23 00:00:00pages
41-6issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579304003114journal_volume
564pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The reversible folding/unfolding of a short peptide in solution is studied by molecular dynamics simulations. The effects of long-range electrostatic interactions are examined and found to be important both for the equilibrium between folded and unfolded states and the dynamics of the folding process. The neglect of l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01079-0
更新日期:1999-09-03 00:00:00
abstract::The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01309-7
更新日期:2003-12-18 00:00:00
abstract::We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00670-4
更新日期:1997-07-07 00:00:00
abstract::Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80644-1
更新日期:1989-06-05 00:00:00
abstract::Strigolactones are a new class of phytohormones synthesized from carotenoids via carlactone. The complex structure of carlactone is not easily deducible from its precursor, a cis-configured β-carotene cleavage product, and is thus formed via a poorly understood series of reactions and molecular rearrangements, all cat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12593
更新日期:2017-03-01 00:00:00
abstract::For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.04.035
更新日期:2012-06-04 00:00:00
abstract::The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that plays a major role in energy sensing. AMPK is activated pharmacologically by 5-amino-4-imidazolecarboxamide (AICA) riboside monophosphate (ZMP), which mimics the effects of AMP on the AMPK cascade. Here we show that uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03501-9
更新日期:2002-11-06 00:00:00
abstract::Expression of the leptin receptor gene has been examined in mouse hypothalamus and other brain regions by in situ hybridization. With a probe recognizing all the known splice variants, receptor mRNA was evident in several brain regions (cortex, hippocampus, thalamus), with strong expression in the hypothalamus (arcuat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00473-5
更新日期:1996-06-03 00:00:00
abstract::Intracellular accumulation of anthracycline derivatives was measured in a human embryonic kidney cell line (HEK) and a resistant subline (HEK/multidrug resistance protein (MRP1)) overexpressing MRP1 at the plasma membrane surface. Two compounds (daunorubicin and doxorubicin) were rejected outside the multidrug-resista...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02270-0
更新日期:2001-03-23 00:00:00
abstract::A potent inhibitor of a dual-specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during a screening of microbial metabolites. This inhibitor was identified as 4-isoavenaciolide (4-iA), and was determined to irreversibly inhibit VHR phosphatase activity with a 50% inhibitory concentration of 1.2 mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03065-x
更新日期:2002-08-14 00:00:00
abstract::Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.026
更新日期:2011-10-03 00:00:00
abstract::The effects of mastoparan on phospholipase C-catalysed phosphoinositide hydrolysis were examined in [3H]inositol-labelled human neuroblastoma SH-SY5Y cells. [3H]Inositol phosphate formation in intact cells was not altered by 20 microM mastoparan. In contrast, [3H]inositol phosphate formation in electrically permeabili...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81366-3
更新日期:1989-04-24 00:00:00
abstract::Polymyxin B (PXB), a cyclic peptide antibiotic, in concentrations 0.1-3.0 mg/ml (0.08-4.0 mmol/l), inhibited the K+ efflux induced by opening of the Ca2+-activated K+ channel (the Gárdos effect) in intact human red blood cells. The inhibition was observed when the Gárdos effect was elicited by Ca2+ in the presence of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81152-3
更新日期:1987-12-10 00:00:00
abstract::Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13259
更新日期:2018-11-01 00:00:00
abstract::Two different isoforms of subunit VIIa have been found in cytochrome c oxidase isolated from human skeletal muscle. The first 22 residues of the N-terminal amino acid sequences showed 5 differences. Our results provide the first conclusive evidence for the existence of cytochrome c oxidase isoenzymes in man. Since the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81376-y
更新日期:1990-04-24 00:00:00
abstract::Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00444-e
更新日期:1995-05-29 00:00:00
abstract::The photocycle of the light-adapted purple membrane was studied with a time-resolved fluorometry apparatus: fluorescence of the sample suspension (greater than 660 nm) was pumped with a 633-nm cw laser and the temporal change induced by a 532-nm pulsed laser was measured with a photon-counting-type transient recorder....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80643-u
更新日期:1992-06-22 00:00:00
abstract::Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.009
更新日期:2013-06-27 00:00:00
abstract::The tailspike protein (TSP) of bacteriophage P22 is a homotrimeric multifunctional protein responsible for cell attachment and hydrolysis of the Salmonella typhimurium host cell receptor. Despite the folding of TSP involves the formation of thermolabile intermediates, the mature protein is extremely resistant to heat ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00876-x
更新日期:1998-08-07 00:00:00
abstract::It was recently shown that there is a predominance of phase 1 introns near the cleavage site of signal peptides encoded by human genes. It was suggested that this biased distribution was due to intron insertion at AGmid R:G proto-splice sites. However, we found that there is no disproportional excess of AGmid R:G that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.094
更新日期:2006-03-06 00:00:00
abstract::We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.048
更新日期:2006-05-29 00:00:00
abstract::We previously reported that tau protein kinase II (TPKII) from bovine brain was composed of 30 kDa and 23 kDa subunits. The 30 kDa subunit of TPKII can be regarded as a catalytic subunit because of its ATP-binding activity. Antibodies directed against TPKII-phosphorylated tau also reacted with tau phosphorylated by cd...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80723-8
更新日期:1993-12-06 00:00:00
abstract::The thermal stability of PQQ glucose dehydrogenases (PQQGDHs) which were chimeras with more than 95% made up of the N-terminal region of Escherichia coli PQQGDH and the rest made up of the C-terminal region of Acinetobacter calcoaceticus PQQGDH was investigated. Among the chimeric PQQGDHs, E97A3 (E. coli 97% and A. ca...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00418-9
更新日期:1995-05-15 00:00:00
abstract::A recent report of electrical measurements on oriented bacteriorhodopsin in gels [(1986) FEBS Lett. 195, 164 168] concluded that low concentrations of diamines reversed the direction of the proton pump. Calculations are presented which show that in low diamine concentrations, charge displacements of the counterion atm...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80775-5
更新日期:1987-05-25 00:00:00
abstract::The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12987
更新日期:2018-02-01 00:00:00
abstract::The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01214-8
更新日期:1996-11-25 00:00:00
abstract::The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00537-6
更新日期:1996-07-01 00:00:00
abstract::Infantile and juvenile neuronal ceroid lipofuscinosis (NCLs) are progressive neurodegenerative disorders of childhood with distinct ages of clinical onset, but with a similar pathological outcome. Infantile and juvenile NCL are inherited in an autosomal recessive manner due to mutations in the CLN1 and CLN3 genes, res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00162-5
更新日期:2003-03-13 00:00:00
abstract::Almost all eukaryotic mRNAs acquire a poly(A) tail at the 3'-end by a concerted RNA processing event: cleavage and polyadenylation. The canonical PAP, PAPα, was considered the only nuclear PAP involved in general polyadenylation of mRNAs. A phosphoinositide-modulated nuclear PAP, Star-PAP, was then reported to regulat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.05.029
更新日期:2014-06-27 00:00:00
abstract::c-Myc is a transcriptional regulator involved in carcinogenesis through its role in growth control and cell cycle progression. Here we attempt to relate its role in stimulating the G1-S transition to the ability to affect functioning of key cell cycle regulators, and we focus on how its property of modulating transcri...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02118-4
更新日期:2001-02-16 00:00:00