Abstract:
:The structures of the M110 and M21 regulatory subunits of protein phosphatase-1M, the major enzyme which dephosphorylates myosin in smooth muscle, have been deduced from cloned cDNAs. The N-terminus of the M110 subunit from rat aorta contains seven ankyrin repeats, while the C-terminus of the M21 subunit from chicken gizzard contains a leucine zipper motif. The M110 subunit is expressed in two different forms which differ in their C-terminal sequences. One of these is highly homologous to the whole of the M21 subunit.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chen YH,Chen MX,Alessi DR,Campbell DG,Shanahan C,Cohen P,Cohen PTdoi
10.1016/0014-5793(94)01231-8subject
Has Abstractpub_date
1994-12-12 00:00:00pages
51-5issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)01231-8journal_volume
356pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80653-7
更新日期:1988-03-28 00:00:00
abstract::The amino acid sequence of rat ribosomal protein L26 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2-terminal amino acid sequence of the protein. Rat L26 contains 145 amino acids and has a molecular mass of 17,266 Da. Hybridization of the cDNA to digests of nuclear DNA sug...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81434-6
更新日期:1989-07-17 00:00:00
abstract::A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80964-8
更新日期:1983-08-22 00:00:00
abstract::Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80049-9
更新日期:1991-08-05 00:00:00
abstract::A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01377-6
更新日期:1995-12-27 00:00:00
abstract::Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81039-o
更新日期:1992-08-10 00:00:00
abstract::Oligonucleotides 5'-d(CT)5-L-d(AG)5-L-d(GA)5-3' and 5'-d(GA)5-L-d(TC)5-L-d(GA)5-3' [L = pO(CH2CH2O)3p] were studied by thermal denaturation, chemical modification and binding of fluorescent dyes. Both oligonucleotides are shown to fold back on itself twice forming at pH 7 a sufficiently stable triplex ether with antip...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00519-f
更新日期:1995-06-19 00:00:00
abstract::A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81053-1
更新日期:1989-09-11 00:00:00
abstract::The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.023
更新日期:2015-01-30 00:00:00
abstract::Heme is essential and synthesized via highly regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins noncovalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such as c-type cytochrome...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.13006
更新日期:2018-03-01 00:00:00
abstract::Ovulation is a complex process initiated by the surge of the pituitary luteinizing hormone (LH) that provokes the expression of specific genes. We report herein the isolation and characterization of an ovulation-associated, ovary-specific novel isoform of epoxide hydrolase 2 (Ephx2), Ephx2C. This variant is exclusivel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.016
更新日期:2007-10-16 00:00:00
abstract::In the presence of ammonium sulfate the absorption spectra of a peroxidase from the fungus Arthromyces ramosus (ARP) showed that the low-spin component increased as the pH increased from 6.0 to 9.0, whereas in its absence ARP remained in the high-spin state in the pH range investigated. The crystal structure of ARP at...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01458-6
更新日期:1996-01-15 00:00:00
abstract::To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted ami...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00277-g
更新日期:1995-04-17 00:00:00
abstract::Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02498-x
更新日期:2001-06-01 00:00:00
abstract::Antibodies generated against synthetic peptides that correspond to highly conserved sequence motifs in the annexins reacted with a variety of annexins from different species. These include Xenopus laevis and Drosophila melanogaster, which contain cross-reacting polypeptides of apparent Mr 34,000 and 30,000. As expecte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81668-0
更新日期:1989-12-04 00:00:00
abstract::Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 ser...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80397-5
更新日期:1985-09-02 00:00:00
abstract::Cytochrome c oxidase has been isolated from beef heart mitochondria and labeled with the fluorochrome eosin-5-maleimide (EMA) after pretreatment with mersalyl. On SDS-polyacrylamide gels, EMA fluorescence and absorption occurred at a single band corresponding to subunit III. Since only Cys 115 of the two cysteinyl res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80664-5
更新日期:1985-05-06 00:00:00
abstract::Proline-rich tyrosine kinase2 (Pyk2) is a cytoplasmic tyrosine kinase related to focal adhesion kinase. Pyk2 expression has been known to be restricted to neuronal and hematopoietic cells and Pyk2 tyrosine phosphorylation and its kinase activity is important for the function of monocytes/macrophages. In NB4 acute prom...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.001
更新日期:2008-02-06 00:00:00
abstract::We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01407-r
更新日期:1995-01-23 00:00:00
abstract::In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81331-8
更新日期:1982-12-13 00:00:00
abstract::A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80638-3
更新日期:1983-03-21 00:00:00
abstract::Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03059-9
更新日期:2001-11-16 00:00:00
abstract::STAT4 is a transcription factor activated in response to IL-12, and is involved in Th1 cell development. The molecular mechanisms controlling the transcription of the STAT4 gene are however, unclear. Sequence comparison of the 5' flanking regions of human, mouse and pufferfish (Fugu rubripes) Stat4 genes revealed a hi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.018
更新日期:2005-08-15 00:00:00
abstract::Methicillin-resistant clinical isolates of Staphylococcus aureus are intrinsically resistant to beta-lactam antibiotics in that the resistance mechanism is unrelated to the possession of beta-lactamases. We have demonstrated that a new, high-molecular-mass penicillin-binding protein (PBP) is present in these strains w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80036-3
更新日期:1985-11-11 00:00:00
abstract::Prominin-1 (CD133) is a cholesterol-interacting pentaspan membrane protein concentrated in plasma membrane protrusions. In epithelial cells, notably neuroepithelial stem cells, prominin-1 is found in microvilli, the primary cilium and the midbody. These three types of apical membrane protrusions are subject to remodel...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.050
更新日期:2010-05-03 00:00:00
abstract::Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to inves...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.068
更新日期:2006-01-23 00:00:00
abstract::It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00097-x
更新日期:1996-02-26 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in plants. In Arabidopsis, 20 MAPKs have been identified and classified into four major groups (A-D). Little is known about the role of group C MAPKs. We have studied the activation of Arabidopsis subgroup C1 MAPKs (A...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.075
更新日期:2007-05-01 00:00:00
abstract::Hyperthermia such as that occurring during fever may improve cell survival during infection, although its mechanism of action is largely unknown. Here we show that acute exposure to mild, but not severe, heat shock induces the synthesis of cyclin D1 that plays a critical role(s) in G1 progression of the cell cycle. Th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02459-6
更新日期:2002-03-27 00:00:00
abstract::Jeotgalicoccus sp. 8456 OleTJE (CYP152L1) is a fatty acid decarboxylase cytochrome P450 that uses hydrogen peroxide (H2 O2 ) to catalyse production of terminal alkenes, which are industrially important chemicals with biofuel applications. We report enzyme fusion systems in which Streptomyces coelicolor alditol oxidase...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12581
更新日期:2017-03-01 00:00:00