Aminoacyl-coenzyme A synthesis catalyzed by a CoA ligase from Penicillium chrysogenum.

Abstract:

:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the proteinogenic amino acids L-phenylalanine and L-tyrosine, as well as the non-proteinogenic amino acids D-phenylalanine, D-tyrosine and (R)- and (S)-β-phenylalanine were also accepted. Of these amino acids, the highest activity was found for (R)-β-phenylalanine, forming (R)-β-phenylalanyl-CoA. Homology modeling suggested that alanine 312 is part of the active site cavity, and mutagenesis (A312G) yielded a variant that has an enhanced catalytic efficiency with β-phenylalanines and D-α-phenylalanine.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

doi

10.1016/j.febslet.2011.02.018

subject

Has Abstract

pub_date

2011-03-23 00:00:00

pages

893-8

issue

6

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(11)00119-0

journal_volume

585

pub_type

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