Abstract:
:We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. This protein is heat stable and has no effect on the other catalytic activities in proteasome, or on the activities of trypsin, chymotrypsin, or m- and mu-calpains either. The molar ratio of inhibitor-to-proteasome that inhibits 50% of the ZLLL-MCA degrading activity of proteasome is 1:1. The inhibitory mechanism of the inhibitor against proteasome is non-competitive. Finally, the inhibitor was identified as heat-shock protein 90 (HSP90) by partial amino acid sequencing and immunodetection. The results suggest that HSP90 initiates neurite outgrowth through the inhibition of the ZLLL-MCA degrading activity in proteasome.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tsubuki S,Saito Y,Kawashima Sdoi
10.1016/0014-5793(94)00388-2subject
Has Abstractpub_date
1994-05-16 00:00:00pages
229-33issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00388-2journal_volume
344pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Rats were infused intravenously with a bolus of mevalonolactone or cholesterol-rich chylomicron and microsomes prepared from the livers at times up to 2 h after the infusion. Acyl-CoA cholesterol acyltransferase (ACAT) activity was measured in the microsomes under conditions that define the activity state of the enzym...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80410-4
更新日期:1983-08-08 00:00:00
abstract::To determine the regulatory mechanism for human tyrosine hydroxylase, we examined modulations of the activity of the enzyme from human pheochromocytoma by cyclic AMP-dependent protein kinase, calmodulin-dependent protein kinase II and polyanion. The most remarkable activation was observed when the enzyme was assayed a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80927-5
更新日期:1989-08-14 00:00:00
abstract::The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80150-1
更新日期:1983-03-07 00:00:00
abstract::We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.080
更新日期:2007-05-29 00:00:00
abstract::Data are presented on the formation of a linoleic acid oxygenation product under basal conditions by guinea pig alveolar macrophages (AM). Under non-stimulated conditions 20 X 10(6) AM released about 1 nmol 9-hydroxylinoleic acid (9-HODE) during a 15 min incubation. Furthermore, AM released more than 2 nmol 12-hydroxy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81121-8
更新日期:1986-12-15 00:00:00
abstract::Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12280
更新日期:2016-08-01 00:00:00
abstract::Mutations in the human mtDNA gene encoding subunit III of cytochrome c oxidase (CO) have been reported to cause MELAS and LHON. Poracoccus denitrificans cells expressing substitutions homologous to these MELAS- and LHON-causing mutations had lower growth yield than wild type cells and lower efficiency of proton pumpin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00891-6
更新日期:1998-08-14 00:00:00
abstract::Even though the plant photoreceptors cryptochromes were discovered more than 20 years ago, the mechanism through which they transduce light signals to their partner molecules such as COP1 (Constitutive Photomorphogenic 1) or SPA1 (Suppressor of Phytochrome A) still remains to be established. We propose that a negative...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.12.008
更新日期:2015-01-16 00:00:00
abstract::Adaptation to hypoxia is essential for tumor progression. Transcriptional activation of hypoxia-regulated genes is mediated by hypoxia-inducible factor 1 (HIF-1), a heterodimer of HIF-1alpha and ARNT (Ah receptor nuclear translocator; HIF-1beta). Using representational difference analysis, we identified three novel hy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03475-0
更新日期:2002-10-23 00:00:00
abstract::The Hippo-Warts pathway defines a novel signaling cascade involved in organ size control and tumor suppression. However, the developmental function of this pathway is less understood. Here we report that the Caenorhabditis elegans homolog of Warts, Ce-wts-1, plays important roles during worm development. The null alle...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.002
更新日期:2009-10-06 00:00:00
abstract::Putrescine N-methyltransferase (PMT) catalyses S-adenosylmethionine (SAM)-dependent methylation of putrescine in tropane alkaloid biosynthesis. PMT presumably evolved from the ubiquitous spermidine synthase (SPDS). SPDS protein structure suggested that only few amino acid exchanges in the active site were necessary to...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.09.043
更新日期:2009-10-20 00:00:00
abstract::Regulatory T-cells (Tregs) are the guardians of peripheral tolerance acting to prevent autoimmune diseases such as systemic lupus erythomatosus (SLE) and rheumatoid arthritis (RA). Defects in Tregs have been reported in these two diseases despite significant differences in their clinical phenotype and pathogenesis. In...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.07.043
更新日期:2011-12-01 00:00:00
abstract::Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81039-o
更新日期:1992-08-10 00:00:00
abstract::It was of interest to examine whether yeast Cu-thionein could be used to transfer the thiolate bound copper directly into the copper binding site of 'blue' apoproteins which contain free thiol groups. In particular apo-stellacyanin was used in the present study and it was found to be able to accept Cu(I) from yeast Cu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80489-x
更新日期:1983-02-07 00:00:00
abstract::Artificial spider silk proteins may form fibers with exceptional strength and elasticity. Wrapping silk, or aciniform silk, is the toughest of the spider silks, and has a very different protein composition than other spider silks. Here, we present the characterization of an aciniform protein (AcSp1) subunit named W1, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.08.024
更新日期:2013-10-01 00:00:00
abstract::The peroxisome assembly factor-1 (PAF-1) is reported here to contain the signature subsequence for a ring finger motif in its carboxyl-terminus. This conserved subsequence in PAF-1 may be the key to a gene expression regulatory pathway important in peroxisome biogenesis. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81397-5
更新日期:1992-11-02 00:00:00
abstract::Endoplasmic reticulum stress (ER-stress) is associated with ataxia telangiectasia mutated (ATM) gene. We present here conclusive data showing that ATM blocks ER-stress induced by tunicamycin or ionizing radiation (IR). X-box protein-1 (XBP-1) splicing, GRP78 expression and caspase-12 activation were increased by tunic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.002
更新日期:2009-03-04 00:00:00
abstract::Exposure of Ehrlich ascites tumor cells to 5-azacytidine for 5 h resulted in a partial loss of ability of DNA to stimulate ADP-ribosyltransferase activity, as assessed in a reconstituted in vitro enzyme system consisting of purified calf thymus enzyme, calf thymus whole histone and DNA isolated from the cells. The deg...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80154-b
更新日期:1990-03-12 00:00:00
abstract::Bacterial cells containing the ner gene of phage Mu inserted into pBR322 express a binding activity with specificity for the left-end EcoRI.C fragment of Mu DNA. Crude extracts containing either Mu repressor or ner protein have been used to localize binding sites on TaqI subfragments of the EcoRI.C fragment. There are...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81128-4
更新日期:1983-06-27 00:00:00
abstract::Rats bearing the fast-growing AH-130 Yoshida ascites hepatoma showed a marked cachectic response which has been previously reported [Tessitore et al. (1987) Biochem. J. 241, 153-159]. Thus tumour-bearing animals showed significant decreases in body and muscle weight (soleus and gastrocnemius) as compared to both pair-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80290-4
更新日期:1994-02-07 00:00:00
abstract::Neuronal activity is often associated with changes in gene expression. By a two-dimensional cDNA-display system, restriction landmark cDNA scanning, we identified a novel gene whose expression in the hippocampus was up-regulated by kainate stimulation. The mRNA expression was detected only in brain and up-regulated by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00497-9
更新日期:1998-05-22 00:00:00
abstract::Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local fluid shear stress. Because the Leu33Pro (Pl(A)) polymorphism of integrin beta(3) confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00170-4
更新日期:2003-04-10 00:00:00
abstract::MicroRNA-107 (miR-107) has been demonstrated to regulate proliferation and apoptosis in many types of cancers. Nevertheless, its biological function in gastric cancer remains largely unexplored. Here, we found that the expression level of miR-107 was increased in gastric cancer in comparison with the adjacent normal t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.12.009
更新日期:2014-02-14 00:00:00
abstract::A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80387-i
更新日期:1991-04-09 00:00:00
abstract::A new peptide, Tc1, containing only 23 amino acids closely packed by three disulfide bridges was isolated from the Amazonian scorpion Tityus cambridgei. It blocks reversibly the Shaker B K(+)-channels with a K(d) of 65 nM and displaces binding of noxiustoxin to mouse brain synaptosome membranes. It is the shortest kno...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02253-5
更新日期:2000-12-08 00:00:00
abstract::There is evidence that CCK-8 may interact with opioids and that both systems are probably implicated in pain modulation. In order to elucidate this relationship we sought to examine factors governing the movement of CCK-8 from the spinal cord into the extracellular space. We report that CCK-8 like immunoreactivity, as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80723-9
更新日期:1989-07-03 00:00:00
abstract::The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific bindi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.05.028
更新日期:2015-07-08 00:00:00
abstract::Disulfide bond formation is a critical step in the folding of many secretory proteins. In bacteria, disulfide bonds are introduced by the periplasmic dithiol oxidase DsbA, which transfers its catalytic disulfide bond to folding polypeptides. Reduced DsbA is reoxidized by ubiquinone Q8, catalyzed by inner membrane quin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.07.063
更新日期:2008-10-15 00:00:00
abstract::We analysed the biochemical properties of insulin receptors of a Type A insulin resistant patient with a single heterozygous point mutation substituting Gln for Arg1174. Insulin binding capacity and affinity to Epstein-Barr virus transformed lymphocytes was normal. Quantitative analysis of autophosphorylation and subs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00876-0
更新日期:1994-09-05 00:00:00
abstract::We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.12.005
更新日期:2013-01-31 00:00:00