Abstract:
:Rats were infused intravenously with a bolus of mevalonolactone or cholesterol-rich chylomicron and microsomes prepared from the livers at times up to 2 h after the infusion. Acyl-CoA cholesterol acyltransferase (ACAT) activity was measured in the microsomes under conditions that define the activity state of the enzyme which recent evidence suggests may depend on the degree of phosphorylation of the enzyme. ACAT activity was increased by both treatments. Some of the observed increase could be attributed to an increase in the supply of substrate cholesterol to the enzyme. The remaining change in activity was consistent with a change in the activity state of ACAT occurring in vivo in response to the influx of cholesterol to the enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Suckling KE,Stange EF,Dietschy JMdoi
10.1016/0014-5793(83)80410-4subject
Has Abstractpub_date
1983-08-08 00:00:00pages
29-32issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(83)80410-4journal_volume
159pub_type
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