ATM blocks tunicamycin-induced endoplasmic reticulum stress.

abstract：:In fission yeast, the only known essential function of Ned8p is the modification of the cullin, Pcu1p, and subsequent Cullin-RING-Ligase (CRL) activation and substrate ubiquitination. We show here that a functional Pcu1p mutant, deleted for its C-terminal autoinhibitory domain, which negates the requirement of neddyla...

journal_title：FEBS letters

authors： Girdwood D,Robertson M,Gordon C

更新日期：2012-05-21 00:00:00

abstract：:Stromal cell-derived factor 1alpha (SDF-1alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this study, we show that SDF-1alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory a...

journal_title：FEBS letters

authors： Ohtsuki T,Hosono O,Kobayashi H,Munakata Y,Souta A,Shioda T,Morimoto C

更新日期：1998-07-17 00:00:00

abstract：:The androgen-binding protein (ABP) has been purified 87,500-fold from rat testis using 4 steps of HPLC, with a yield of 14%. The molecule was 99% pure with a specific activity estimated to 16,600 pmol/mg protein. The iodinated molecule was eluted in 2 peaks in Sephacryl S300 gel filtration with a molecular mass estima...

journal_title：FEBS letters

authors： Gueant JL,Khanfri J,Gerard H,Fremont S,Gerard A,Grignon G,Nicolas JP

更新日期：1986-10-27 00:00:00

abstract：:Constitutively secreted proteins have traditionally been believed to be excluded from the regulated secretory pathway. In this work we show that kappa light chain and Fc fragment, two markers of the constitutive pathway, are present in the regulated pathway in AtT-20 cells. They colocalize with the endogenous hormone ...

journal_title：FEBS letters

authors： Castle AM,Huang AY,Castle JD

更新日期：1998-11-20 00:00:00

abstract：:Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO3)2 in phosphate buffer, while the activity decreased only 20% if the incubation was...

journal_title：FEBS letters

authors： Dimroth P,Thomer A

更新日期：1992-03-23 00:00:00

abstract：:Redox homeostasis is a prerequisite for survival of the pathogen Mycobacterium tuberculosis (Mtb) which employs the low molecular weight thiol mycothiol (MSH). The Mycobacterial NADPH-dependent mycothione reductase (MtMtr), composed of an NADPH-, FAD-, and a dimerization-domain connected by linkers, regulates the bala...

journal_title：FEBS letters

authors： Kumar A,Subramanian Manimekalai MS,Grüber G

更新日期：2018-02-01 00:00:00

abstract：:The role of the Ca(2+)-activated tyrosine kinase, Pyk2, in the pleiotropic coupling of nerve cell stimulation to the MAP kinase cascade still remains undefined. Using a panel of PC12 clones, one of which was defective in Pyk2, we demonstrate (1) that the MAP kinase response induced by a [Ca(2+)](i) rise (following app...

journal_title：FEBS letters

authors： Barsacchi R,Heider H,Girault J,Meldolesi J

更新日期：1999-11-19 00:00:00

abstract：:Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific st...

journal_title：FEBS letters

authors： Efremov RG,Nolde DE,Volynsky PE,Chernyavsky AA,Dubovskii PV,Arseniev AS

更新日期：1999-11-26 00:00:00

abstract：:We searched for the previously defined uncoupling protein (UCP) signatures [Jezek, P. and Urbánková, E. (2000) IUBMB Life 49, 63-70] in genomes of Drosophila melanogaster, Caenorhabditis elegans, Dictyostelium discoideum, and Arabidopsis thaliana. We identified four UCPs in Drosophila and one in Caenorhabditis or Dict...

journal_title：FEBS letters

authors： Hanák P,Jezek P

更新日期：2001-04-27 00:00:00

abstract：:Rhodopsin phosphorylation was investigated using synthetic C-terminal peptides from rhodopsin. The peptides were phosphorylated by expressed rhodopsin kinase (RK) in the presence of a photolyzed truncated rhodopsin at the C-terminus. No peptide phosphorylation was detected under dark or in conditions in which RK was i...

journal_title：FEBS letters

authors： Ohguro H

更新日期：1997-08-25 00:00:00

abstract：:Production of glycerol and a key enzyme in glycerol production, glycerol 3-phosphate dehydrogenase (NAD+) (GPD), was studied in Saccharomyces cerevisiae cultured in basal media or media of high salinity, with glucose, raffinose or ethanol as the sole carbon source. At high salinity, glycerol production was stimulated ...

journal_title：FEBS letters

authors： André L,Hemming A,Adler L

更新日期：1991-07-29 00:00:00

abstract：:Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

journal_title：FEBS letters

authors： Amano T,Tozawa K,Yoshida M,Murakami H

更新日期：1994-07-04 00:00:00

abstract：:The amounts of RNA polymerase I (Pol I) and basal rDNA transcription factors were determined in yeast whole cell extracts. A 17-fold excess of Pol I was found compared to the Pol I-specific initiation factors upstream activating factor (UAF) and core factor (CF) which underlines that both initiation factors interact w...

journal_title：FEBS letters

authors： Bier M,Fath S,Tschochner H

更新日期：2004-04-23 00:00:00

abstract：:alphaArg-376, betaLys-155, and betaArg-182 are catalytically important ATP synthase residues that were proposed to be involved in substrate Pi binding and subsequent steps of ATP synthesis [Senior, A.E., Nadanaciva, S. and Weber, J. (2002) Biochim. Biophys. Acta 1553, 188-211]. Here, it was shown using purified Escher...

journal_title：FEBS letters

authors： Ahmad Z,Senior AE

更新日期：2005-01-17 00:00:00

abstract：:Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric oxide reacts with hy...

journal_title：FEBS letters

authors： Noronha-Dutra AA,Epperlein MM,Woolf N

更新日期：1993-04-19 00:00:00

abstract：:Addition of cyanide to the CO complex of cytochrome oxidase reduces the apparent photosensitivity of the Fe-CO bond. This effect is not seen with azide, or when cyanide is added to ferromyoglobin-CO. It is proposed that cyanide binds to CuB, and restricts the passage of CO out of the protein. This restriction favors g...

journal_title：FEBS letters

authors： Hill BC

更新日期：1994-11-14 00:00:00

abstract：:Brain nitric oxide synthase is a Ca2+/calmodulin-regulated enzyme which converts L-arginine into NO. Enzymatic activity of this enzyme essentially depends on NADPH and is stimulated by tetrahydrobiopterin (H4biopterin). We found that purified NO synthase contains enzyme-bound H4biopterin, explaining the enzymatic acti...

journal_title：FEBS letters

authors： Mayer B,John M,Heinzel B,Werner ER,Wachter H,Schultz G,Böhme E

更新日期：1991-08-19 00:00:00

abstract：:Human legumain was characterized following overexpression in a murine cell line as the C-terminal Ig-fusion protein. Upon acid treatment, the prolegumain autoproteolyzed distal to two aspartic acid residues to yield a highly active form. The ability of mature legumain to cleave after aspartic acid residues was confirm...

journal_title：FEBS letters

authors： Halfon S,Patel S,Vega F,Zurawski S,Zurawski G

更新日期：1998-10-30 00:00:00

abstract：:Rapidly generated high-titer Semliki Forest virus (SFV) vectors can infect numerous mammalian cell lines and primary cell cultures, and result in high levels of transgene expression. SFV-based expression of transmembrane receptors has been characterized by specific ligand-binding activity and functional responses. Ada...

journal_title：FEBS letters

authors： Lundstrom K,Schweitzer C,Rotmann D,Hermann D,Schneider EM,Ehrengruber MU

更新日期：2001-08-31 00:00:00

abstract：:DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...

journal_title：FEBS letters

authors： Wang Y,Rea T,Bian J,Gray S,Sun Y

更新日期：1999-02-26 00:00:00

abstract：:The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

journal_title：FEBS letters

authors： Budohoski L,Challiss RA,McManus B,Newsholme EA

更新日期：1984-02-13 00:00:00

abstract：:We present the three-dimensional structure of the N-terminal FK506-binding protein (FKBP)-like domain of the immunophilin FKBP42 from Arabidopsis thaliana. The data provide the structural background for the explanation of key functional properties reported previously. ...

journal_title：FEBS letters

authors： Weiergräber OH,Eckhoff A,Granzin J

更新日期：2006-01-09 00:00:00

abstract：:Here we show that increased amount of secondary structure is acquired in the folded states of two structurally-different proteins (alpha-helical VlsE and alpha/beta flavodoxin) in the presence of macromolecular crowding agents. The structural content of flavodoxin and VlsE is enhanced by 33% and 70%, respectively, in ...

journal_title：FEBS letters

authors： Perham M,Stagg L,Wittung-Stafshede P

更新日期：2007-10-30 00:00:00

abstract：:In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...

journal_title：FEBS letters

authors： Greenhalf W,Stephan C,Chaudhuri B

更新日期：1996-02-12 00:00:00

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

journal_title：FEBS letters

authors： Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

更新日期：2004-01-02 00:00:00

abstract：:Integral cGMP-dependent currents as well as activity of single cGMP-activated channels in plasma membrane of rod outer segment (ROS) were recorded using the patch-clamp method. The dependence of integral currents on cGMP concentration is shown to be bell-shaped. Decrease in cGMP-dependent conductance at high cGMP conc...

journal_title：FEBS letters

authors： Zhainazarov AB,Kolesnikov SS

更新日期：1990-01-15 00:00:00

abstract：:A serum-free medium conditioned by U937, a human cell line of monocyte/macrophage origin, was found capable of inducing apoptosis on exponentially growing U937 cells themselves (autocrine suicide). The apoptosis-inducing agent is a macromolecule and possibly a protein (SKT factor), with a relative molecular mass in th...

journal_title：FEBS letters

authors： Ghibelli L,Coppola S,Nosseri C,Bergamini A,Beninati S

更新日期：1994-05-09 00:00:00

abstract：:The stimulatory effects of several DNA-binding basic proteins (histone and protamine) and HIV-1 Rev with arginine (Arg)-rich clusters on the activity of casein kinase II (CK-II) were investigated in vitro. It was found that recombinant Rev (rRev) and the synthetic oligo-fragments corresponding to the amino acid sequen...

journal_title：FEBS letters

authors： Ohtsuki K,Maekawa T,Harada S,Karino A,Morikawa Y,Ito M

更新日期：1998-05-29 00:00:00

abstract：:The ability of a yeast nuclear protein to be transported into the nucleus of a higher eukaryotic cell was investigated. Mcm1, a transcriptional activator protein from Saccharomyces cerevisiae, was microinjected into the cytoplasm of Xenopus laevis frog oocytes. Mcm1 was imported into the oocyte nucleus indicating that...

journal_title：FEBS letters

authors： Wagner P,Hall MN

更新日期：1993-04-26 00:00:00