Crystal structure of a plant immunophilin domain involved in regulation of MDR-type ABC transporters.

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:Affinity labelling of pancreatic RNase with 4-(N-2-chloroethyl-N-methylamino)benzylamide and (N----P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as sugg...

journal_title：FEBS letters

authors： Knorre DG,Buneva VN,Baram GI,Godovikova TS,Zarytova VF

更新日期：1986-01-01 00:00:00

abstract：:All acetylcholine receptor subunit genes contain E boxes and are blocked by membrane depolarization. We have used transfected C2C12 myogenic cells to investigate the response, to electrical stimulation and KCl, of wildtype and mutant regulatory regions of the chick acetylcholine receptor alpha, gamma and delta subunit...

journal_title：FEBS letters

authors： Su CT,Huang CF,Schmidt J

更新日期：1995-06-12 00:00:00

abstract：:Systemic AA-amyloidosis is a complication of chronic inflammatory diseases and the fibril protein AA derives from the acute phase reactant serum AA. AA-amyloidosis can be induced in mice by an inflammatory challenge. The lag phase before amyloid develops can be dramatically shortened by administration of a small amoun...

journal_title：FEBS letters

authors： Westermark GT,Westermark P

更新日期：2009-08-20 00:00:00

abstract：:We have previously published a simple kinetic model to analyse possible pitfalls in kinetic measurements of H+/O ratios in mitochondria [(1984) FEBS Lett. 178, 187-192]. While this model demonstrated how relative electrode response times may affect the results, it did not adequately describe the kinetics of proton bac...

journal_title：FEBS letters

authors： Krab K,Wikström M

更新日期：1986-06-09 00:00:00

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:Tumour necrosis factor-alpha (TNFalpha) is a multifunctional cytokine that exerts a myriad of biological actions in numerous different tissues including adipocytes through its two distinct cell surface receptors. To address the role of each TNF receptor in the biological actions of TNFalpha in adipocytes, we have deve...

journal_title：FEBS letters

authors： Sethi JK,Xu H,Uysal KT,Wiesbrock SM,Scheja L,Hotamisligil GS

更新日期：2000-03-03 00:00:00

abstract：:Treatment of the myeloid cell lines, U-937 or HL-60, with 10 nM of the phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), for 24 h increased the rate of incorporation of [3H]glycerol into total chloroform extracts. A proportionally greater labeling of the non-polar lipid (NL) fraction compared to the polar, ph...

journal_title：FEBS letters

authors： Ponzoni M,DeBell KE,Hoffman T,Bonvini E

更新日期：1990-12-10 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

journal_title：FEBS letters

authors： Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

更新日期：1997-07-21 00:00:00

abstract：:Challenge of Rhodobacter capsulatus cells with the superoxide propagator methyl viologen resulted in the induction of a diaphorase activity identified as a member of the ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate (NADP(H)) reductase (FPR) family by N-terminal sequencing. The gene cod...

journal_title：FEBS letters

authors： Bittel C,Tabares LC,Armesto M,Carrillo N,Cortez N

更新日期：2003-10-23 00:00:00

abstract：:Octopus, squid and cuttle-fish organs were examined for alcohol dehydrogenase activity. Only one form was detectable, with properties typical of mammalian class III alcohol dehydrogenase. The corresponding protein was purified from octopus and enzymatically characterized. Ion-exchange and affinity chromatography produ...

journal_title：FEBS letters

authors： Rosario Fernández M,Jörnvall H,Moreno A,Kaiser R,Parés X

更新日期：1993-08-16 00:00:00

abstract：:A PCR-based subtractive cloning procedure was used to identify genes expressed at higher levels in the pancreatic beta cell line betaTC1, as compared to the pancreatic alpha cell line alphaTC1. One of the clones isolated by this procedure corresponded to the regulatory subunit (RIalpha) of protein kinase A (PKA). Usin...

journal_title：FEBS letters

authors： Arava Y,Adamsky K,Belleli A,Shaltiel S,Walker MD

更新日期：1998-03-20 00:00:00

abstract：:Tau exon 10 (E10) splicing is a crucial event in its developmental change of tau isoform and tauopathy. To investigate the splicing mechanism, we isolated and compared mouse tau genomic sequence with human sequence. We identified a new element in mouse intron 10 (I10) to suppress E10 splicing, which was located just a...

journal_title：FEBS letters

authors： Yamashita T,Tomiyama T,Li Q,Numata H,Mori H

更新日期：2005-01-03 00:00:00

abstract：:Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to...

journal_title：FEBS letters

authors： Berger MR,Alvarado R,Kiss DL

更新日期：2019-04-01 00:00:00

abstract：:Blebs of the sarcoplasmic reticulum (SR) membrane of heart muscle cells were generated after saponin perforation of the plasma membrane followed by complete hypercontraction of the cell. Although characteristic proteins of the plasma membrane, namely the beta1-adrenoreceptor and Galphai, were stained by monoclonal ant...

journal_title：FEBS letters

authors： Böhle T,Brandt MC,Henn N,Schmidt A,Bloch W,Beuckelmann DJ

更新日期：2001-09-21 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:To better understand the outcome of the interaction between TNF-related apoptosis-inducing factor (TRAIL) and tumor cells, we studied TRAIL-resistant melanoma cells resulting from prolonged exposure to TRAIL and found that they had higher proliferative activity than the parental cells both in vitro and in vivo. This w...

journal_title：FEBS letters

authors： Wu JJ,Zhang XD,Gillespie S,Hersey P

更新日期：2005-03-28 00:00:00

abstract：:The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elem...

journal_title：FEBS letters

authors： Lavrentieva I,Broude NE,Lebedev Y,Gottesman II,Lukyanov SA,Smith CL,Sverdlov ED

更新日期：1999-01-29 00:00:00

abstract：:Plasmids for the high-level overproduction of wild-type, and C- and N-terminal His-tagged MurG N-acetylglucosaminyl transferase from Escherichia coli were constructed. In complementation tests the three forms were active in vivo. After IPTG induction, growth, spheroplast formation and lysis, overproduced MurG proteins...

journal_title：FEBS letters

authors： Crouvoisier M,Mengin-Lecreulx D,van Heijenoort J

更新日期：1999-04-23 00:00:00

abstract：:Inflammation is highly regulated by various agents. Unexpectedly, we report here that the damage-associated molecular pattern S100A9 protein, a potent neutrophil activator and inducer of cytokine production in monocytes, is not a direct activator of cytokine production in human neutrophils. However, S100A9 primed IL-8...

journal_title：FEBS letters

authors： Simard JC,Noël C,Tessier PA,Girard D

更新日期：2014-06-13 00:00:00

abstract：:Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

journal_title：FEBS letters

authors： Evans MC,Gourovskaya K,Nugent JH

更新日期：1999-05-07 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:Human Bfl-1 is an anti-apoptotic Bcl-2 family member. Here, we found that Bfl-1 was converted into a potent death-promoting protein by green fluorescent protein (GFP) fusion with its N-terminus. The transient expression of GFP-Bfl-1 induced cytochrome c release and triggered apoptosis in 293T cells, which depended on ...

journal_title：FEBS letters

authors： Ko JK,Choi KH,Kim HJ,Choi HY,Yeo DJ,Park SO,Yang WS,Kim YN,Kim CW

更新日期：2003-09-11 00:00:00

abstract：:This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

journal_title：FEBS letters

authors： Hatse S,Princen K,Bridger G,De Clercq E,Schols D

更新日期：2002-09-11 00:00:00

abstract：:Phosphate triester derivatives of the anti-neoplastic alkyl lyso phospholipid (ALP) have been prepared as novel potential therapeutic agents. In particular, symmetrical phosphate triesters have been prepared, using phosphorochloridate chemistry. The compounds have been fully characterised by a range of techniques, and...

journal_title：FEBS letters

authors： McGuigan C,Wang Y,Riley PA

更新日期：1995-09-25 00:00:00

abstract：:Recently, we reported two novel early-staged encapsulation-relating proteins (56 kDa and 48 kDa ERPs) isolated from the hemolymph of coleopteran insect, Tenebrio molitor larvae [Cho et al. (1999) Eur. J. Biochem. (in press)]. Here, a cDNA clone for another early-staged encapsulation-relating protein (86 kDa) was isola...

journal_title：FEBS letters

authors： Cho MY,Choi HW,Moon GY,Kim MH,Kwon TH,Homma K,Natori S,Lee BL

更新日期：1999-05-28 00:00:00

abstract：:Several protein kinase C (PKC) isoforms are expressed in human platelets. We report that PKC-delta is tyrosine phosphorylated within 30 s of platelet activation by thrombin. This correlated with a 2-3-fold increase in the kinase activity of PKC-delta relative to unstimulated platelets. The tyrosine phosphorylated PKC-...

journal_title：FEBS letters

authors： Moussazadeh M,Haimovich B

更新日期：1998-11-06 00:00:00

abstract：:In Clostridium thermoaceticum, the synthesis of acetyl-CoA from methyl tetrahydrofolate occurs via a series of enzymatic reactions involving methyl transferase, corrinoid/Fe-S protein (corrinoid), carbon monoxide dehydrogenase (CODH) and ferredoxin. We have investigated the possibility of one or more of these proteins...

journal_title：FEBS letters

authors： Shanmugasundaram T,Sundaresh CS,Kumar GK

更新日期：1993-07-12 00:00:00

abstract：:The products of the isiAB operon are a chlorophyll antenna protein (IsiA) and flavodoxin (IsiB), which accumulate in cyanobacteria grown under iron starvation conditions. Here we show that strong light triggers de-repression of isiAB transcription and leads to IsiA and flavodoxin accumulation under iron replete condit...

journal_title：FEBS letters

authors： Havaux M,Guedeney G,Hagemann M,Yeremenko N,Matthijs HC,Jeanjean R

更新日期：2005-04-25 00:00:00