Abstract:
:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at the extreme C-terminus. This sorting signal operates independently of targeting information in IST2 mRNA and sorting to the plasma membrane does not require She-mediated mRNA transport into daughter cells. Based on these results, we suggest a posttranslational mechanism, which leads to the concentration of Ist2--via multimerization--at ER sites, followed by direct transport to the plasma membrane. This novel mechanism operates downstream of IST2 mRNA localization.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Franz A,Maass K,Seedorf Mdoi
10.1016/j.febslet.2006.12.048subject
Has Abstractpub_date
2007-02-06 00:00:00pages
401-5issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00011-7journal_volume
581pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Leishmania major promastigotes are parasites endowed with a plasma membrane electrogenic H+ pump and anionic channels. These systems have been thought to contribute to pH homeostasis of parasites and environmental adaptation by mediating extrusion of protons which are either generated metabolically or result from exog...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00171-5
更新日期:1995-03-13 00:00:00
abstract::The metallochromic indicator murexide has been used to monitor calcium concentration changes during the dextran-induced, phosphatidylserine-dependent degranulation of rat peritoneal mast cells. The dextran-induced Ca2+-uptake showed an absolute dependence on the presence of phosphatidylserine. The extent of Ca2+-uptak...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80725-x
更新日期:1983-09-05 00:00:00
abstract::Tumor necrosis factor alpha (TNF-α) is a prototypical proinflammatory cytokine that can elicit strong inflammation in macrophages by activating NF-κB. The underlying epigenetic mechanism is obscure. We show here that megakaryocytic leukemia 1 (MKL1) is an epigenetic mediator of TNF-α-induced proinflammatory transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12601
更新日期:2017-03-01 00:00:00
abstract::In addition to their g = 1.94 EPR signal, nitrogenase Fe-proteins from Azotobacter vinelandii, Azotobacter chroococcum and Klebsiella pneumoniae exhibit a weak EPR signal with g approximately equal to 5. Temperature dependence of the signal was consistent with an S = 3/2 system with negative zero-field splitting, D = ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81033-4
更新日期:1985-09-23 00:00:00
abstract::Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.017
更新日期:2014-03-03 00:00:00
abstract::Escherichia coli maltose-binding protein (MBP) is frequently used as an affinity tag to facilitate the purification of recombinant proteins. An important additional attribute of MBP is its remarkable ability to enhance the solubility of its fusion partners. MBPs are present in a wide variety of microorganisms includin...
journal_title:FEBS letters
pub_type: 历史文章,杂志文章
doi:10.1016/s0014-5793(03)00070-x
更新日期:2003-02-27 00:00:00
abstract::HepG2 cells, stably transfected with MDR1 cDNA, encoding the P-glycoprotein multidrug resistance efflux pump, display an altered sphingolipid composition compared to control cells, stably transfected with empty vector. The MDR1 overexpressing cells display a approximately 3-fold increased level of lactosylceramide and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.003
更新日期:2005-06-20 00:00:00
abstract::A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensiona...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00473-m
更新日期:1995-05-29 00:00:00
abstract::Glycogen phosphorylase b at concentrations close to those found in skeletal muscle interacts with sarcoplasmic reticulum membranes, but not with liposomes made of lipids extracted from these membranes, and is inhibited upon binding to the membrane. The interaction of glycogen phosphorylase with the sarcoplasmic reticu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80606-4
更新日期:1991-06-03 00:00:00
abstract::Fatty acids induce apoptosis in primary astrocytes by enhancing ceramide synthesis de novo. The possible role of the AMP-activated protein kinase (AMPK) in the control of apoptosis was studied in this model. Long-term stimulation of AMPK with 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) prevented apoptosis. A...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02089-0
更新日期:2001-02-02 00:00:00
abstract::In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the me...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.050
更新日期:2005-02-28 00:00:00
abstract::Polypeptides rich in glutamic acid are strong inhibitors purified from isolated nuclei of Xenopus laevis oocytes of casein kinase II. The presence of tyrosine in these peptides greatly enhances their inhibitory capacity. Using casein as a substrate, copolyglu:tyr (4:1) has an I50 value of 20 nM, 250 fold lower than th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80897-r
更新日期:1990-06-04 00:00:00
abstract::The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81237-2
更新日期:1989-04-10 00:00:00
abstract::The Fanconi anemia (FA) pathway, of which the FANCD2 protein is a key component, plays crucial roles in the maintenance of hematopoietic stem cells and suppression of carcinogenesis. However, the function of FANCD2 remains unclear. Here, we report that FANCD2 is a novel and specific substrate of caspase 3. Cleavage of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.08.027
更新日期:2014-10-16 00:00:00
abstract::FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02915-5
更新日期:2001-10-19 00:00:00
abstract::Mutations in the presenilin (PS) genes PSI and PS2 are involved in Alzheimer's disease (AD). Recently, apoptosis-associated cleavage of PS proteins was identified. Here we demonstrate that PS1 as well as PS2 are substrates for different members of the caspase protein family. Remarkably, the caspases acting on PS1 coul...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00108-8
更新日期:1999-02-19 00:00:00
abstract::A complete cDNA encoding rabbit uteroglobin was constructed and expressed in MDCK and Caco-2 cells. The MDCK cells secrete uteroglobin in approximately equal amounts to the apical and the basolateral side, whereas the Caco-2 cells secrete uteroglobin mainly to the basolateral side. Both MDCK and Caco-2 cells thus secr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80891-w
更新日期:1993-09-20 00:00:00
abstract::The heterotrophic nitrifier Paracoccus denitrificans expresses a membrane-associated ammonia monooxygenase. The active enzyme has been solubilized in the detergent dodecyl-beta-D-maltoside and purified by standard chromatographic techniques. This is the first purification of an ammonia monooxygenase. The enzyme consis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00463-2
更新日期:1996-05-27 00:00:00
abstract::Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13679
更新日期:2020-03-01 00:00:00
abstract::The Mahogany/Attractin gene (Atrn) has been proposed as a downstream mediator of Agouti signaling because yellow hair color and obesity in lethal yellow (A(y)) mice are suppressed by the mahogany (Atrn(mg)) mutation. The present study examined the distribution of Atrn mRNA in the brain and spinal cord by in situ hybri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01494-5
更新日期:1999-11-26 00:00:00
abstract::Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80131-2
更新日期:1994-03-07 00:00:00
abstract::The human gene frataxin and its yeast homolog YFH1 affect mitochondrial function. Deficits in frataxin result in Friedreich ataxia, while deletion of YFH1 results in respiratory incompetence. We determined that as long as respiratory incompetent yeast express Yfh1p they do not accumulate excessive mitochondrial iron. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03137-4
更新日期:2001-11-30 00:00:00
abstract::The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00956-8
更新日期:1996-09-30 00:00:00
abstract::The cAMP-dependent induction of 2,5-oligoadenylate (2-5A) synthetase and cAMP-dependent inhibition of 2-5A phosphodiesterase are shown. Variations in activities of cAMP-dependent protein kinase and the enzymes of 2-5A metabolism in the cells deepening into the resting state were found to be compatible with the above f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80467-6
更新日期:1984-06-04 00:00:00
abstract::A site-selective cAMP analog, 8-chloroadenosine 3',5'-cyclic monophosphate (8-Cl-cAMP), was demonstrated to be a potent inhibitor of both the monolayer and soft agar growth of normal rat kidney (NRK) fibroblasts that had been transformed with the v-Ki-ras oncogene or treated with transforming growth factor alpha (TGF ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80502-2
更新日期:1989-01-02 00:00:00
abstract::The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01067-o
更新日期:1995-10-16 00:00:00
abstract::We characterized the promoter activity of a medaka fish intestinal guanylyl cyclase gene, OlGC6, by assay of enzyme activity in response to various promoter-luciferase fusion gene constructs introduced into CACO-2 cells and medaka fish embryos. A transient transfection assay of the various fusion gene constructs showe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00002-4
更新日期:2003-02-11 00:00:00
abstract::In mammalian cells, the Bcl-2 and Bcl-x(L) proteins suppress programmed cell death whereas the topographically similar Bax protein accelerates the apoptotic process. Recently published data suggest that expression of the human Bax-alpha gene is lethal for the yeast Saccharomyces cerevisiae and that this toxicity can b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00044-0
更新日期:1996-02-12 00:00:00
abstract::Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80856-e
更新日期:1990-12-17 00:00:00
abstract::The membrane-membrane fusion during fertilization of oocyte by spermatozoa is believed to be mainly mediated by so called "fusion proteins". In the present study we have tried to demonstrate that beside the proteins, lipid components of membrane may play an important role in fusion of oocyte with spermatozoa. Conventi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.015
更新日期:2006-04-17 00:00:00