Abstract:
:Affinity labelling of pancreatic RNase with 4-(N-2-chloroethyl-N-methylamino)benzylamide and (N----P) N-methylimidazolide of d(pTpA) results in the formation of monomodified enzyme derivatives retaining partially enzymatic activity. These data together with some cases described in the literature are considered as suggesting the dynamic nature of the enzyme-reagent complex represented by a set of states differing in the probability of intra-complex reaction. In particular, modification may proceed in a low probability state with an especially favorable mutual orientation of reagent and some protein residue remote from the active site of the enzyme resulting in the removal of the covalently attached reagent moiety from the active center.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Knorre DG,Buneva VN,Baram GI,Godovikova TS,Zarytova VFdoi
10.1016/0014-5793(86)80052-7subject
Has Abstractpub_date
1986-01-01 00:00:00pages
64-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(86)80052-7journal_volume
194pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::P67, a new protein binding to a specific RNA probe, was purified from radish seedlings [Echeverria, M. and Lahmy, S. (1995) Nucleic Acids Res. 23, 4963-4970]. Amino acid sequence information obtained from P67 microsequencing allowed the isolation of genes encoding P67 in radish and Airabidopsis thaliana. Immunolocalis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01935-9
更新日期:2000-09-01 00:00:00
abstract::Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12040
更新日期:2016-01-01 00:00:00
abstract::The manganese containing center of the oxygen evolving complex accumulates four oxidizing equivalents in the four stepped water oxidation cycle. Based on experiments on electrochromic absorption transients and the reduction rate of the primary electron donor, P680, it has been speculated that the oscillations of these...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01181-8
更新日期:1994-11-21 00:00:00
abstract::In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.058
更新日期:2006-12-22 00:00:00
abstract::The catalytic cycle of heme peroxidases involves two reactive states, compound I and compound II. Although their reduction potentials at pH 7 are similar, compound I is in general more reactive towards organic substrates than compound II. The different reactivities have until now remained unexplained. In this study, t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00792-8
更新日期:1997-07-28 00:00:00
abstract::We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.042
更新日期:2006-05-29 00:00:00
abstract::The tissue inhibitors of matrix metalloproteinases (MMPs), TIMP-1 and TIMP-2, are also angiogenesis inhibitors. Cathepsin B and MMPs are found at sites of neovascularization in pathologies such as cancer and osteoarthritis. Treatment of TIMP-1, TIMP-2, and of a mixture of both inhibitors from human articular chondrocy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00897-2
更新日期:1999-07-23 00:00:00
abstract::A cDNA of a type 7 adenylyl cyclase isoform was cloned from a bovine retinal pigment epithelium cDNA library using oligonucleotides developed to conserved regions common to mammalian adenylyl cyclases. A 6.7 kb mRNA of very high abundance was uniquely present on Northern blots containing mRNA or total RNA from the pig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01470-5
更新日期:1996-01-15 00:00:00
abstract::Creatine kinases (CK) play a prominent role in cell energy distribution through an energy shuttle between mitochondria and other organelles. Human brain CK was cloned and overexpressed in COS-7 cells. We then deleted His-65 and/or Pro-66 situated near the center of a flexible loop as shown by X-ray crystallography on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01614-8
更新日期:2000-06-09 00:00:00
abstract::In vitro cultured blood cells for transfusion purposes provide a safe alternative to donor blood, particularly for patients who require recurrent transfusions, and can be used as carriers of therapeutic molecules. In vitro derivation of hematopoietic cell types from human-induced pluripotent stem cells (iPSCs) allows ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13599
更新日期:2019-12-01 00:00:00
abstract::In Alzheimer's disease, beta-amyloid peptides (betaA(1-40) and betaA(1-42)) are deposited on the brain cell surfaces as neurotoxic plaques. Some reports indicate that small heat shock proteins, Hsp27 and alphaB-crystallin, colocalize in the plaques, but their functions are not known. Interaction between betaA and alph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02136-0
更新日期:2000-11-03 00:00:00
abstract::Viperin is a radical SAM enzyme that possesses antiviral properties against a broad range of enveloped viruses. Here, we describe the activity of human viperin with two molecules of the mevalonate pathway, geranyl pyrophosphate, and farnesyl pyrophosphate, involved in cholesterol biosynthesis. We postulate that the ra...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12941
更新日期:2018-01-01 00:00:00
abstract::In order to better define the structural elements involved in allosteric signalling, wild-type DnaK and three deletion mutants of the peptide binding domain have been characterized by biophysical (steady-state and time-resolved fluorescence) and biochemical methods. In the presence of ATP the chemical environment of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03752-3
更新日期:2003-01-02 00:00:00
abstract::The neopterin derivatives, neopterin and 7,8-dihydroneopterin, modulate the cellular oxidant-antioxidant balance as well as the expression of the inducible nitric oxide synthase (iNOS) gene. Since apoptosis can be induced by reactive oxygen intermediates and nitric oxide (NO) we investigated whether these neopterin de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01194-5
更新日期:1996-11-18 00:00:00
abstract::Inhibition of mammalian DNA topoisomerase I by phospholipids was investigated using purified enzyme. Acidic phospholipids inhibited the DNA relaxation activity of topoisomerase I whereas neutral phospholipid, phosphatidylethanolamine, did not. Accumulation of a protein-DNA cleavable complex, an intermediate which is k...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80658-6
更新日期:1990-02-12 00:00:00
abstract::The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant bl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.11.031
更新日期:2007-12-11 00:00:00
abstract::In Alzheimer's disease (AD) brain, microtubule-associated protein tau is abnormally modified by hyperphosphorylation and glycosylation, and is aggregated as neurofibrillary tangles of paired helical filaments. To investigate the role of tau glycosylation in neurofibrillary pathology, we isolated various pools of tau p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02228-7
更新日期:2002-02-13 00:00:00
abstract::The modification of homeodomain-interacting protein kinase 2 (HIPK2) by small ubiquitin-like modifier 1 (SUMO-1) plays an important role in its targeting into the promyelocytic leukemia body, as well as in its differential interaction with binding partner, but the desumoylation of HIPK2 by SUMO-specific proteases is l...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.10.010
更新日期:2005-11-07 00:00:00
abstract::The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02639-4
更新日期:2001-08-03 00:00:00
abstract::Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.03.074
更新日期:2004-05-07 00:00:00
abstract::Anomalously high values of photoinduced absorption changes were revealed in the antenna of photosynthetic purple bacteria. They were found to be 4-16 times greater at the bleaching peak of the antenna than at the bleaching peak of the BChl dimer of the reaction center. This is direct proof of excitation delocalization...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00663-t
更新日期:1995-07-17 00:00:00
abstract::Flash excitation of Tris-treated photosystem II (PSII) membranes generated a thermoluminescence band peaking at -20 degrees C, indicating that a positively charged oxidizing equivalent is stably accumulated on donor side of PSII even in the absence of the Mn-cluster. This oxidizing equivalent was sensitive to low conc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80112-g
更新日期:1991-01-28 00:00:00
abstract::HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80649-f
更新日期:1993-10-18 00:00:00
abstract::The CA microsatellite repeat marker, D7S522, is located at the center of a approximately 1000 kb smallest common deleted region that is lost in many forms of human cancer. It has been proposed that a putative tumor suppressor gene lies in close proximity to D7S522, within this smallest common deleted region. However, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00365-8
更新日期:1999-04-09 00:00:00
abstract::We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80469-w
更新日期:1992-05-25 00:00:00
abstract::Phosphorylation of the basal site with glycogen synthase kinase-4 enhanced the rate of phosphorylation of the regulatory site by cyclic AMP-dependent protein kinase 1.7-fold. In contrast, the phosphorylation state of the regulatory site did not affect the rate of phosphorylation of the basal site with glycogen synthas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80390-9
更新日期:1988-05-09 00:00:00
abstract::The photoreduction of the primary electron acceptor, QA, has been characterized by light-induced Fourier transform infrared difference spectroscopy for Rb. sphaeroides reaction centers and for Rsp. rubrum and Rp. viridis chromatophores. The samples were treated both with redox compounds, which rapidly reduce the photo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80129-q
更新日期:1991-01-28 00:00:00
abstract::Apart from the 8 kDa subunit, which is of chloroplast origin, most of the small polypeptides of the PS I reaction center from higher plants are encoded in nuclear genes. We describe here the first nucleotide sequence of a nuclear component of this photosystem, the precursor of the 20 kDa protein. The deduced sequence ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80752-x
更新日期:1988-05-23 00:00:00
abstract::One of the most intriguing plant defense reactions against herbivores is the emission of volatiles as potentially attractive signals for the natural enemies of the attacking species. Like many other low and high molecular weight chemical defenses, volatile production is under the control of the octadecanoid signalling...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01398-9
更新日期:1995-12-27 00:00:00
abstract::Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80784-x
更新日期:1984-10-01 00:00:00