Abstract:
:Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hait NC,Fujita K,Lester RL,Dickson RCdoi
10.1016/s0014-5793(02)03636-0keywords:
subject
Has Abstractpub_date
2002-12-04 00:00:00pages
97-102issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579302036360journal_volume
532pub_type
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