Abstract:
:Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family of cytokines that promotes apoptosis and NF-kappaB activation. Here we show that recombinant hu-TRAIL initiates the activation of multiple caspases, the loss of mitochondrial transmembrane potential, the cleavage of BID and the redistribution of mitochondrial cytochrome c. However, whereas Bcl-2 efficiently blocked UV radiation-induced cytochrome c release and consequent apoptosis of CEM cells, it failed to do either in the context of TRAIL treatment. Thus, TRAIL engages a death pathway that is at least partially routed via the mitochondria, but in contrast with other stimuli that engage this pathway, TRAIL-induced cytochrome c release is not regulated by Bcl-2.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Keogh SA,Walczak H,Bouchier-Hayes L,Martin SJdoi
10.1016/s0014-5793(00)01375-2keywords:
subject
Has Abstractpub_date
2000-04-07 00:00:00pages
93-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01375-2journal_volume
471pub_type
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