Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets.

abstract：:Tau phosphorylation plays a crucial role in microtubule stabilization and in Alzheimer's disease. To characterize the molecular mechanisms of tau binding on microtubules, we synthesized the peptide R1 (QTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQI), reproducing the first tau microtubule binding motif. We thermodynamically charac...

journal_title：FEBS letters

authors： Devred F,Douillard S,Briand C,Peyrot V

更新日期：2002-07-17 00:00:00

abstract：:5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natur...

journal_title：FEBS letters

authors： Rossi AG,Thomas MJ,O'Flaherty JT

更新日期：1988-11-21 00:00:00

abstract：:A novel type IV collagen-degrading metalloproteinase was purified from the conditioned media of a murine metastatic sarcoma cell line. The molecular weight of the purified enzyme was determined to be 100 kDa by SDS-PAGE, while 700 kDa by gel filtration suggesting that the enzyme has a multimer structure. This enzyme d...

journal_title：FEBS letters

authors： Kato Y,Ogawa K,Yamamoto S,Abe S,Kishi J,Hayakawa T

更新日期：1990-07-30 00:00:00

abstract：:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold,...

journal_title：FEBS letters

authors： McEachern F,Fisher LM

更新日期：1989-08-14 00:00:00

abstract：:By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...

journal_title：FEBS letters

authors： Kambe T,Murakami M,Kudo I

更新日期：1999-06-18 00:00:00

abstract：:The oxysterols, 7beta-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7Beta-hydroxycholesterol and...

journal_title：FEBS letters

authors： Lizard G,Lemaire S,Monier S,Gueldry S,Néel D,Gambert P

更新日期：1997-12-15 00:00:00

abstract：:Leucine-rich repeats (LRRs) are 22-28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide deri...

journal_title：FEBS letters

authors： Gay NJ,Packman LC,Weldon MA,Barna JC

更新日期：1991-10-07 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:The function of the amino- and carboxyl-terminal domains of the yeast plasma membrane H+-ATPase have been investigated by constructing deletions in vitro and selectively expressing the mutant enzymes in vivo. The first 27 amino acids are dispensable but deletion of a further 33 amino acids greatly decreases the appear...

journal_title：FEBS letters

authors： Portillo F,de Larrinoa IF,Serrano R

更新日期：1989-04-24 00:00:00

abstract：:The 21-residue peptide KLLLLKLLLLKLLLLKLLLLK (KL4) has been synthesized and analyzed regarding its secondary structure and orientation in lipid environments. Fourier transform infrared and circular dichroism spectroscopy shows that the peptide exhibits approximately 80% alpha-helical content both in dodecylphosphochol...

journal_title：FEBS letters

authors： Gustafsson M,Vandenbussche G,Curstedt T,Ruysschaert JM,Johansson J

更新日期：1996-04-15 00:00:00

abstract：:We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...

journal_title：FEBS letters

authors： Fernández A

更新日期：1992-02-03 00:00:00

abstract：:Thimet oligopeptidase (EC 3.4.24.15; TOP) is a Zn(II) endopeptidase implicated in physiological regulation of processes involving neuropeptides. The present study clarifies the active site structure and mechanism of catalysis of TOP. The enzyme exhibited a bell-shaped pH dependence of activity having an acidic limb du...

journal_title：FEBS letters

authors： Sigman JA,Edwards SR,Pabon A,Glucksman MJ,Wolfson AJ

更新日期：2003-06-19 00:00:00

abstract：:Activation of AMP-activated protein kinase (AMPK) has been viewed as an important target for the treatment of insulin resistance. Here, by proteomic analysis, we found that expression of heat shock protein beta-1 (HSPB1) was induced by the AMP analog 5-aminoimidazole-4-carboxamide 1-β-D-ribofuranoside in palmitate-ind...

journal_title：FEBS letters

authors： Yuan H,Wang T,Niu Y,Liu X,Fu L

更新日期：2017-01-01 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:The concentration of caffeine required to elute wholly single-stranded DNA from benzoylated DEAE-cellulose is proportional to the polynucleotide length. The use of benzoylated DEAE-cellulose chromatography for isolating and sizing single-stranded regions in double-stranded DNA has been examined using a series of hybri...

journal_title：FEBS letters

authors： Norris MD,Stewart BW

更新日期：1988-02-15 00:00:00

abstract：:Light stimulation of rod cells in vertebrate eyes may cause Ca2+ release from the intracellular disks. Radiolabelled tracers show that light causes a small hyperpolarization of intact disk stacks and redistribution of the ions Ca2+ and Cl-. ...

journal_title：FEBS letters

authors： Hughes SM,Brand MD

更新日期：1985-03-25 00:00:00

abstract：:A functional rat heart Na(+)-Ca2+ exchanger gene has been obtained by splicing and ligating two partially overlapping clones isolated from a rat heart lambda ZAP cDNA library. The deduced primary structure of the protein encoded by the open reading frame corresponds to 971 amino acids, that can be organized into 12 tr...

journal_title：FEBS letters

authors： Low W,Kasir J,Rahamimoff H

更新日期：1993-01-18 00:00:00

abstract：:Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...

journal_title：FEBS letters

authors： Hornstein E,Harel H,Levy G,Meyuhas O

更新日期：1999-08-27 00:00:00

abstract：:SUPERMAN was identified as a putative regulator of transcription that acts in floral development, but its function remains to be clarified. We demonstrate here that SUPERMAN is an active repressor whose repression domain is located in the carboxy-terminal region. Ectopic expression of SUPERMAN that lacked the repressi...

journal_title：FEBS letters

authors： Hiratsu K,Ohta M,Matsui K,Ohme-Takagi M

更新日期：2002-03-13 00:00:00

abstract：:Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...

journal_title：FEBS letters

authors： Giambanco I,Bianchi R,Ceccarelli P,Pula G,Sorci G,Antonioli S,Bocchini V,Donato R

更新日期：1991-09-23 00:00:00

abstract：:Dimethylsulphoxide (DMSO) reductase from R. capsulatus contains a molybdenum-pterin cofactor at its active site. As prepared the molybdenum is in the 6+ oxidation state, devoid of EPR signals. Stepwise reduction generates an EPR signal characteristic of Mo(V) having hyperfine coupling to a single proton and integratin...

journal_title：FEBS letters

authors： Benson N,Farrar JA,McEwan AG,Thomson AJ

更新日期：1992-07-28 00:00:00

abstract：:A mouse somatostatin (SS) receptor cDNA was cloned from neuroblastoma x glioma (NG108-15) cells. The sequence is almost identical to that of the mouse SSTR2 receptor [(1992) Proc. Natl. Acad. Sci. USA 89, 251)] but lacks about 300 nucleotides between transmembrane domain VII and the C-terminus. This spliced variant of...

journal_title：FEBS letters

authors： Vanetti M,Kouba M,Wang X,Vogt G,Höllt V

更新日期：1992-10-26 00:00:00

abstract：:Parts of the amino acid sequences of horse and dromedary pancreatic ribonuclease were reinvestigated. The sequence of residues 21-25 in horse ribonuclease is Ser-Asn-Pro-Thr-Tyr or Ser-Asn-Ser-Thr-Tyr. The asparagine in the latter sequence is glycosylated. Horse ribonuclease possesses four additional amino acid residu...

journal_title：FEBS letters

authors： Beintema JJ

更新日期：1985-06-03 00:00:00

abstract：:The G-protein regulatory (GPR) motif is a approximately 25 amino acid sequence that stabilizes the GDP-bound conformation of Gialpha. To identify additional GPR motifs, we expanded a motif-based search strategy and identified an additional 4 mammalian proteins (WAVE1-3, rat GHRH) and 10 plant proteins with candidate G...

journal_title：FEBS letters

authors： Song KS,Peterson YK,Freidman A,Blumer JB,Sato M,Lanier SM

更新日期：2006-04-03 00:00:00

abstract：:Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...

journal_title：FEBS letters

authors： Otte J,Wruck W,Adjaye J

更新日期：2017-08-01 00:00:00

abstract：:DNA methylation has a profound impact on the regulation of gene expression in normal cell development, and aberrant methylation has been recognized as a key factor in the pathogenesis of human diseases such as cancer. The discovery of modified nucleobases arising from 5-methylcytosine (5mC) through consecutive oxidati...

journal_title：FEBS letters

authors： Dietzsch J,Feineis D,Höbartner C

更新日期：2018-06-01 00:00:00

abstract：:The domains of the pore-forming protein responsible for the gate and channel formations were separated and identified in the outer membrane of Pseudomonas aeruginosa. The proteolytic cleavage of the 46K channel protein, protein D2, yielded two major domains with apparent M(r) of 27K and 19K. We identified the 27K poly...

journal_title：FEBS letters

authors： Yoshihara E,Nakae T

更新日期：1992-07-13 00:00:00

abstract：:Plant Golgi bodies possess unique morphological and functional characteristics that are key to several biological and biotechnological processes, such as transport of the cell's building blocks to energy-rich compartments, including chloroplasts, storage vacuoles and a cellulosic cell wall. During the last decade it h...

journal_title：FEBS letters

authors： Faso C,Boulaflous A,Brandizzi F

更新日期：2009-12-03 00:00:00

abstract：:Lipopolysaccharide, the invariant structural component of Gram-negative bacteria, when present in minute amounts in the circulation in humans elicits 'endotoxic shock' syndrome, which is fatal in 60% of the cases. Polymyxin B (PMB), a cyclic cationic peptide, neutralizes the endotoxin, but also induces many harmful si...

journal_title：FEBS letters

authors： Thomas CJ,Surolia A

更新日期：1999-02-26 00:00:00

abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...

journal_title：FEBS letters

authors： Bucci C,Lütcke A,Steele-Mortimer O,Olkkonen VM,Dupree P,Chiariello M,Bruni CB,Simons K,Zerial M

更新日期：1995-06-05 00:00:00