Mechanism of transport of L-alanine by luminal-membrane vesicles from pars recta of rabbit proximal tubule.

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightl...

journal_title：FEBS letters

authors： Dell'Antone P

更新日期：1994-08-22 00:00:00

abstract：:Formation of tubulin/Na(+),K(+)-ATPase (NKA) complex in erythrocytes of hypertensive subjects results in a 50% reduction in NKA activity. We demonstrate here that detyrosinated tubulin, which is increased in hypertensive erythrocytes membranes, enhances the inhibitory effect of acetylated tubulin on NKA activity. More...

journal_title：FEBS letters

authors： Amaiden MR,Santander VS,Monesterolo NE,Nigra AD,Rivelli JF,Campetelli AN,Pie J,Casale CH

更新日期：2015-01-30 00:00:00

abstract：:Proteins and peptides are readily and specifically modified at their sulfhydryl groups by the vapors of a mixture of 4-vinylpyridine and tributylphosphine. The phenylthiohydantoin derivative of S-beta-(4-pyridylethyl)cysteine formed during sequence analysis is easily detectable in current identification systems. ...

journal_title：FEBS letters

authors： Amons R

更新日期：1987-02-09 00:00:00

abstract：:Treatment of H. chlorum membrane preparations with diethyl ether of high degrees of water saturation raised the bacteriochlorophyll (BChl) g' mole fraction, as determined by HPLC analysis of their acetone extracts, toward a level of 40% of total BChl g or higher. Starting from pure BChl g, the BChl g' mole fraction sh...

journal_title：FEBS letters

authors： Kobayashi M,Watanabe T,Ikegami I,van de Meent EJ,Amesz J

更新日期：1991-06-17 00:00:00

abstract：:Alternative splicing of the Alzheimer's amyloid beta protein precursor (ABPP) message leads to the production of several variants of this precursor polypeptide. Two of these variants contain a domain that is highly homologous to members of the Kunitz class of protease inhibitors. In order to initiate a study of the ph...

journal_title：FEBS letters

authors： Castro M,Marks CB,Nilsson B,Anderson S

更新日期：1990-07-16 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...

journal_title：FEBS letters

authors： Perkins KJ,Davies KE

更新日期：2018-06-01 00:00:00

abstract：:Increases in choline containing metabolites have been associated with a number of disorders, including malignant cell growth. In this study, high resolution magic angle spinning (1)H nuclear magnetic resonance spectroscopy was employed to monitor metabolite changes during cell transfection, and an increase in phosphoc...

journal_title：FEBS letters

authors： Griffin JL,Mann CJ,Scott J,Shoulders CC,Nicholson JK

更新日期：2001-12-07 00:00:00

abstract：:Activation of T-cells infected by HIV-1 results in activation of long terminal repeat (LTR)-dependent viral transcription and ultimately the production of infectious virus. Although full T-cell activation requires a complex series of intracellular signals, including protein kinase C activation, calcium mobilisation, a...

journal_title：FEBS letters

authors： Baldari CT,Macchia G,Massone A,Telford JL

更新日期：1992-06-15 00:00:00

abstract：:We have isolated a portion of the canine gene encoding the orphan receptor RDC1 [1]. The complete coding sequence is contained in a single exon, and an intron divides the 5' untranslated region of RDC1 mRNA. The RDC1 protein is 94% homologous to the gene product of GPRN1, which has been proposed to serve as a VIP rece...

journal_title：FEBS letters

authors： Cook JS,Wolsing DH,Lameh J,Olson CA,Correa PE,Sadee W,Blumenthal EM,Rosenbaum JS

更新日期：1992-03-30 00:00:00

abstract：:Mycobacterium avium subsp. hominissuis (MAH) is an environmental bacterium causing opportunistic infections. The objective of this study was to identify flexible genome regions in MAH isolated from different sources. By comparing five complete and draft MAH genomes we identified a genomic island conferring additional ...

journal_title：FEBS letters

authors： Lahiri A,Sanchini A,Semmler T,Schäfer H,Lewin A

更新日期：2014-11-03 00:00:00

abstract：:Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional clas...

journal_title：FEBS letters

authors： Karar J,Dolt KS,Mishra MK,Arif E,Javed S,Pasha MA

更新日期：2007-10-02 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00

abstract：:Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or Rho...

journal_title：FEBS letters

authors： Anear E,Parish RW

更新日期：2012-05-07 00:00:00

abstract：:Phorbol-esters have been shown to modulate the beta-adrenergic-stimulated adenylyl cyclase in a number of cell lines. Here, using site directed mutagenesis, we investigate the role of the beta 2-adrenergic receptor phosphorylation by protein kinase C in this regulatory process. Mutation of the serine-261, -262, -344 a...

journal_title：FEBS letters

authors： Bouvier M,Guilbault N,Bonin H

更新日期：1991-02-25 00:00:00

abstract：:Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...

journal_title：FEBS letters

authors： Kawade R,Kuroda D,Tsumoto K

更新日期：2020-03-01 00:00:00

abstract：:Cold-stable and cold-labile microtubules were prepared by two cycles of assembly and disassembly and two periods of exposure to cold. The cold-labile preparations were shown to contain a higher proportion of a high molecular mass microtubule-associated protein (MAP 2) than cold-stable preparations. The cold-stable pre...

journal_title：FEBS letters

authors： Hesketh JE

更新日期：1984-04-24 00:00:00

abstract：:A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...

journal_title：FEBS letters

authors： Muñoz A,Harvey R,Carrasco L

更新日期：1983-08-22 00:00:00

abstract：:The hepatitis B viral X protein (HBx) is a multifunctional protein that plays a role in the activation of the NF-kappaB signaling pathway. In this study, we demonstrate that HBx up-regulates expression of TANK-binding kinase-1 (TBK1) at the transcriptional level. Increased levels of TBK1 protein then induce the phosph...

journal_title：FEBS letters

authors： Kim HR,Lee SH,Jung G

更新日期：2010-02-05 00:00:00

abstract：:Steroids are of universal occurrence, present variously as cell wall constituents and bioregulators. A number of bacteria, fungi, and photosynthetic vascular plants synthesize steroids that are hormonally active in the animal world. The cellular effect of such steroids in microbes and plants appears by and large to be...

journal_title：FEBS letters

authors： Agarwal MK

更新日期：1993-05-17 00:00:00

abstract：:The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT-Raman spectroscopy in reaction centres purified from an antenna-deficient strain of Rhodobacter sphaeroides. We find that mutation at the M210 position does not significantly perturb th...

journal_title：FEBS letters

authors： Jones MR,Heer-Dawson M,Mattioli TA,Hunter CN,Robert B

更新日期：1994-02-14 00:00:00

abstract：:Monoclonal H5 antibodies and a polyclonal antiserum, raised against the globular domain of chicken H5 (GH5) but which cross-reacts with histone H1(0) from mouse liver, were used to search for H5 or H1 (0)-like proteins in chicken embryo and adult tissue sections by indirect immunofluorescence. Chicken cell lines in cu...

journal_title：FEBS letters

authors： Shannon MF,Wigley PL,Wells JR

更新日期：1985-07-08 00:00:00

abstract：:Oligonucleotide probes based on the known amino acid sequence of salmon calcitonin were used to screen a cDNA library obtained from ultimobranchial glands of salmon for clones encoding salmon calcitonin. From the cDNA sequence of strongly hybridizing clones the complete primary structure of the calcitonin precursor co...

journal_title：FEBS letters

authors： Pöschl E,Lindley I,Hofer E,Seifert JM,Brunowsky W,Besemer J

更新日期：1987-12-21 00:00:00

abstract：:In order to better define the structural elements involved in allosteric signalling, wild-type DnaK and three deletion mutants of the peptide binding domain have been characterized by biophysical (steady-state and time-resolved fluorescence) and biochemical methods. In the presence of ATP the chemical environment of t...

journal_title：FEBS letters

authors： Moro F,Fernández V,Muga A

更新日期：2003-01-02 00:00:00

abstract：:The structure determination of apotransketolase and the comparison of its three-dimensional structure with that of the holoenzyme has revealed that no large conformational changes are associated with cofactor binding. Two loops at the active site are flexible in the apoenzyme which enables ThDP to reach its binding si...

journal_title：FEBS letters

authors： Sundström M,Lindqvist Y,Schneider G

更新日期：1992-11-30 00:00:00

abstract：:Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...

journal_title：FEBS letters

authors： Brown EM,Redgrave J,Thatcher J

更新日期：1984-09-17 00:00:00

abstract：:In the first committed reaction of chlorophyll biosynthesis, magnesium chelatase couples ATP hydrolysis to the thermodynamically unfavorable Mg(2+) insertion into protoporphyrin IX (ΔG°' of circa 25-33 kJ·mol(-1) ). We explored the thermodynamic constraints on magnesium chelatase and demonstrate the effect of nucleoti...

journal_title：FEBS letters

authors： Adams NB,Brindley AA,Hunter CN,Reid JD

更新日期：2016-06-01 00:00:00

abstract：:In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 wer...

journal_title：FEBS letters

authors： Kregler O,Schilf R,Lander A,Bannert N,Bogner E

更新日期：2009-04-02 00:00:00

abstract：:Two hybrid proteins between Pseudomonas aeruginosa exotoxin A (PE) and poliovirus protease 2Apro have been generated. One hybrid protein contains the poliovirus 2Apro sequence replacing the region of PE corresponding to amino acids 413-607. The other hybrid contains in addition the transforming growth factor sequence....

journal_title：FEBS letters

authors： Novoa I,Feduchi E,Carrasco L

更新日期：1994-11-21 00:00:00

abstract：:Streptococci are a broad group of Gram-positive bacteria. This genus includes various human pathogens causing significant morbidity and mortality. Two of the most important human pathogens are Streptococcus pneumoniae (pneumococcus) and Streptococcus pyogenes (group A streptococcus or GAS). Streptococcal pathogens hav...

journal_title：FEBS letters

authors： Syed S,Viazmina L,Mager R,Meri S,Haapasalo K

更新日期：2020-08-01 00:00:00