Characterization of yeast fructose-2,6-bisphosphate 6-phosphatase.


:To obtain information on the biological significance of yeast fructose-2,6-bisphosphate 6-phosphatase, kinetic data of the purified enzyme [(1987) Eur. J. Biochem. 164, 27-30] have been measured. Maximal activity was found between pH 6 and 7, the apparent Michaelis constant with fructose 2,6-bisphosphate was 7.2 microM at pH 6.0 and 79 microM at pH 7.0. Concentrations required for 50% inhibition of the enzyme at pH 6.0 were 8 microM Fru2P, 45 microM G1c6P, 80 microM Fru6P and 200 microM inorganic phosphate. The known intracellular steady-state level of about 10 microM fructose 2,6-bisphosphate in the presence of glucose is likely to be the result of a balance between the rapid synthesis of fructose 2,6-bisphosphate catalyzed by 6-phosphofructose 2-kinase and a fructose 2,6-bisphosphate degrading activity. The biological function of fructose-2,6-bisphosphate 6-phosphatase with an apparent Michaelis constant between 7 and 79 microM fructose 2,6-bisphosphate at pH 6-7 is therefore suggested to participate in the maintenance of a steady-state level of fructose 2,6-bisphosphate in a concentration range that fits well with the Michaelis constant of the enzyme.




FEBS letters


Plankert U,Purwin C,Holzer H




Has Abstract


1988-10-24 00:00:00














  • Characterization of early induced genes in Arabidopsis thaliana responding to bacterial inoculation: identification of centrin and of a novel protein with two regions related to kinase domains.

    abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cordeiro MC,Piqueras R,de Oliveira DE,Castresana C

    更新日期:1998-09-04 00:00:00

  • Mitochondrial functional interactions between frataxin and Isu1p, the iron-sulfur cluster scaffold protein, in Saccharomyces cerevisiae.

    abstract::Friedreich's ataxia is caused by a deficit in the mitochondrial protein frataxin. The present work demonstrates that in vivo yeast frataxin Yfh1p and Isu1p, the mitochondrial scaffold protein for the Fe-S cluster assembly, have tightly linked biological functions, acting in concert to promote the Fe-S cluster assembly...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ramazzotti A,Vanmansart V,Foury F

    更新日期:2004-01-16 00:00:00

  • Dual specificity antibodies using a double-stranded oligonucleotide bridge.

    abstract::The covalent conjugation of oligonucleotides to antibody Fab' fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chaudri ZN,Bartlet-Jones M,Panayotou G,Klonisch T,Roitt IM,Lund T,Delves PJ

    更新日期:1999-04-30 00:00:00

  • The nucleosome remodeling factor.

    abstract::An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Alkhatib SG,Landry JW

    更新日期:2011-10-20 00:00:00

  • D-Aspartate stimulation of testosterone synthesis in rat Leydig cells.

    abstract::D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nagata Y,Homma H,Lee JA,Imai K

    更新日期:1999-02-12 00:00:00

  • The phosphate recognition site of Escherichia coli maltodextrin phosphorylase.

    abstract::The role of two positively charged amino acid residues located at the active site of Escherichia coli maltodextrin phosphorylase was investigated by site-directed mutagenesis. Substitution of Lys539 by an arginine caused a 600-fold reduction, substitution of Arg534 by a glutamine caused an even larger 7000-fold reduct...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schinzel R,Drueckes P

    更新日期:1991-07-29 00:00:00

  • Kaempferol is an estrogen-related receptor alpha and gamma inverse agonist.

    abstract::Kaempferol is a dietary flavonoid that is thought to function as a selective estrogen receptor modulator. In this study, we established that kaempferol also functions as an inverse agonist for estrogen-related receptors alpha and gamma (ERRalpha and ERRgamma). We demonstrated that kaempferol binds to ERRalpha and ERRg...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wang J,Fang F,Huang Z,Wang Y,Wong C

    更新日期:2009-02-18 00:00:00

  • Links between fatty acids and expression of UCP2 and UCP3 mRNAs.

    abstract::Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Thompson MP,Kim D

    更新日期:2004-06-18 00:00:00

  • Protein-based phylogenetic analysis by using hydropathy profile of amino acids.

    abstract::So far, various approaches for phylogenetic analysis have been developed. Almost all of them put stress on analyzing nucleic acid sequences or protein primary structures. In this paper, we take the physicochemical properties of amino acids into account and introduce the hydropathy profile of amino acids into phylogene...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Liu N,Wang T

    更新日期:2006-10-02 00:00:00

  • Sequence of subunit a of the Na(+)-translocating F1F0-ATPase of Acetobacterium woodii: proposal for residues involved in Na+ binding.

    abstract::Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rahlfs S,Müller V

    更新日期:1999-06-18 00:00:00

  • The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: molecular and physiological characterization of PEPC kinase mutants.

    abstract::Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meimoun P,Gousset-Dupont A,Lebouteiller B,Ambard-Bretteville F,Besin E,Lelarge C,Mauve C,Hodges M,Vidal J

    更新日期:2009-05-19 00:00:00

  • Degradation by the 2',5'-phosphodiesterase activity of mouse cells requires the presence of a ribo hydroxyl group in the penultimate position of the oligonucleotide substrate.

    abstract::A series of 9-beta-D-xylofuranosyladenine (xyloA or xyloadenosine) substituted analogs of 2-5A core trimer and tetramer were examined for their ability to be degraded by the 2',5'-phosphodiesterase activity of cytoplasmic extracts of mouse L cells. Two distinct groups of xyloA-substituted analogs could be readily disc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Torrence PF,Alster D,Huss S,Gosselin G,Imbach JL

    更新日期:1987-02-23 00:00:00

  • On the biosynthesis of free and covalently bound PQQ. Glutamic acid decarboxylase from Escherichia coli is a pyridoxo-quinoprotein.

    abstract::Analysis of glutamic acid decarboxylase (GDC) (EC from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van der Meer RA,Groen BW,Duine JA

    更新日期:1989-03-27 00:00:00

  • Interaction of plasma gelsolin with tropomyosin.

    abstract::Horse plasma gelsolin labelled with benzophenone-4-isothiocyanate can be photochemically cross-linked to rabbit cardiac tropomyosin. The cross-linking proceeds with greater efficiency in calcium-containing buffers. Further evidence for interaction between these proteins is provided by retention of fluorescently labell...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Koepf EK,Burtnick LD

    更新日期:1992-08-31 00:00:00

  • Using lipoate enantiomers and thioredoxin to study the mechanism of the 2-oxoacid-dependent dihydrolipoate production by the 2-oxoacid dehydrogenase complexes.

    abstract::The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

    更新日期:1995-09-04 00:00:00

  • Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins.

    abstract::Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins. In order to understand the molecular basis of these structures, we examined the binding of recombinant human tropoelastin to other extracellular matrix ligands in solid phase bindi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sasaki T,Göhring W,Miosge N,Abrams WR,Rosenbloom J,Timpl R

    更新日期:1999-10-29 00:00:00

  • A proform of secretin with high secretin-like bioactivity.

    abstract::A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gafvelin G,Carlquist M,Mutt V

    更新日期:1985-05-20 00:00:00

  • How the protonation state of a phosphorylated amino acid governs molecular recognition: insights from classical molecular dynamics simulations.

    abstract::Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kawade R,Kuroda D,Tsumoto K

    更新日期:2020-03-01 00:00:00

  • Hirudin or hirudin-like factor - that is the question: insights from the analyses of natural and synthetic HLF variants.

    abstract::The hirudin-like factor 1 (HLF1) of Hirudo medicinalis belongs to a new class of leech-derived factors. In previous investigations, HLF1 did not exhibit anticoagulatory activities. Here, we describe the analysis of natural and synthetic variants of HLF1 and HLF-Hyb, a yet uncharacterized member of the HLF family. Modi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller C,Lukas P,Böhmert M,Hildebrandt JP

    更新日期:2020-03-01 00:00:00

  • Dab1 stabilizes its interaction with Cin85 by suppressing Cin85 phosphorylation at serine 587.

    abstract::Crk and CrkL adaptors play essential neuronal positioning roles downstream of Reelin-induced Dab1 tyrosine phosphorylation. Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1. Here using mass spectrometry, biochemical and mutational...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bior BK,Ballif BA

    更新日期:2013-01-04 00:00:00

  • Human peripheral blood lymphocytes express D5 dopamine receptor gene and transcribe the two pseudogenes.

    abstract::Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two relat...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi N,Nagai Y,Ueno S,Saeki Y,Yanagihara T

    更新日期:1992-12-07 00:00:00

  • Overexpression of cdk4/cyclin D1 induces apoptosis in PC12 cells in the presence of trophic support.

    abstract::The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katayama K,Dobashi Y,Kitagawa M,Kamekura S,Kawai M,Kadoya Y,Kameya T

    更新日期:2001-12-14 00:00:00

  • Thiostrepton binds to malarial plastid rRNA.

    abstract::Binding of the thiazolyl peptide antibiotic thiostrepton to the GTPase domain of 23S rRNA involves a few crucial nucleotides, notably A1067 (E. coli). Small RNA transcripts were prepared corresponding to the GTPase domain of the plastid 23S rRNA and the two forms of cytosolic 28S rRNAs found in the human malaria paras...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Clough B,Strath M,Preiser P,Denny P,Wilson IR

    更新日期:1997-04-07 00:00:00

  • Decreased stability of photosystem I in dgd1 mutant of Arabidopsis thaliana.

    abstract::The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Guo J,Zhang Z,Bi Y,Yang W,Xu Y,Zhang L

    更新日期:2005-07-04 00:00:00

  • Concomitant activation of Gi and Gq protein-coupled receptors does not require an increase in cytosolic calcium for platelet aggregation.

    abstract::U46619 is a potent platelet agonist, its binding to the thromboxane A2 receptor resulting in Gq-binding protein-mediated responses; nevertheless, it is unable to cause platelet aggregation, unless released ADP is present. In this study we demonstrate that Gi activation is the step U46619 lacks to cause platelet aggreg...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pulcinelli FM,Pesciotti M,Pignatelli P,Riondino S,Gazzaniga PP

    更新日期:1998-09-11 00:00:00

  • Robust global sensitivity in multiple enzyme cascade system explains how the downstream cascade structure may remain unaffected by cross-talk.

    abstract::A steady-state framework was applied to the ubiquitous tricyclic enzyme cascade structure, as seen in the mitogen-activated protein (MAP) kinase system, to analyze the effect of upstream kinase concentrations on final output response. The results suggest that signal amplification achieved by the cascade structure ensu...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mutalik VK,Singh AP,Edwards JS,Venkatesh KV

    更新日期:2004-01-30 00:00:00

  • Forskolin induces circadian gene expression of rPer1, rPer2 and dbp in mammalian rat-1 fibroblasts.

    abstract::Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yagita K,Okamura H

    更新日期:2000-01-07 00:00:00

  • Affinity labelling of Escherichia coli ribosomes with a benzylidene derivative of AUGU6 within initiation and pretranslocational complexes.

    abstract::Affinity labelling of E. coli ribosomes with the 2',3'-O-[4-(N-2-chloroethyl)-N-methylamino]benzylidene derivative of AUGU6 was studied within the initiation complex (complex I) obtained by using fMet-tRNAMetf and initiation factors and within the pretranslocational complex (complex II) obtained by treatment of comple...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Babkina GT,Veniaminova AG,Vladimirov SN,Karpova GG,Yamkovoy VI,Berzin VA,Gren EJ,Cielens IE

    更新日期:1986-07-07 00:00:00

  • Comparison of proline and N-methylnorleucine induced conformational equilibria in cyclic pentapeptides.

    abstract::The cyclic, imido acid containing pentapeptides cyclo(Asp-Trp-(NMe)Nle-Asp-Phe) (cpp[NMeNle(3)]) and cyclo(Asp-Trp-Pro-Asp-Phe) (cpp[Pro(3)]) have been investigated by 1H-NMR spectroscopy in DMSO and by restrained molecular dynamics methods. The spectra indicate the existence of at least four cis/trans isomers for cpp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Weisshoff H,Wieprecht T,Henklein P,Frömmel C,Antz C,Mügge C

    更新日期:1996-06-03 00:00:00

  • Microbial pathogenesis revealed by intravital microscopy: pros, cons and cautions.

    abstract::Intravital multiphoton imaging allows visualization of infections and pathogenic mechanisms within intact organs in their physiological context. Today, most organs of mice and rats are applicable to in vivo or ex vivo imaging, opening completely new avenues for many researchers. Advances in fluorescent labeling of pat...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Stolp B,Melican K

    更新日期:2016-07-01 00:00:00