The interaction between the catalytic A subunit of calcineurin and its autoinhibitory domain, in the yeast two-hybrid system, is disrupted by cyclosporin A and FK506.

abstract：:Effects of sulfatide and its precursor galactosylceramide (gal-cer) on the kinetics of production of cytokines were studied. In human mononuclear leucocytes, gal-cer but not sulfatide induced significantly increased amounts of interleukin (IL)-1beta, IL-6 and tumor necrosis factor (TNF) mRNA. In phytohemagglutinin-sti...

journal_title：FEBS letters

authors： Bovin LF,Fredman P,Månsson JE,Buschard K,Bendtzen K

更新日期：1999-07-23 00:00:00

abstract：:The polyomavirus enhancer binding protein 2alpha (PEBP2alpha) is a DNA binding transcriptional regulatory protein that binds conserved sites in the polyomavirus enhancer, mammalian type C retroviral enhancers and T-cell receptor gene enhancers. Binding of PEBP2alpha and homologous proteins to the consensus DNA sequenc...

journal_title：FEBS letters

authors： Pérez-Alvarado GC,Munnerlyn A,Dyson HJ,Grosschedl R,Wright PE

更新日期：2000-03-24 00:00:00

abstract：:The mutant hen egg white lysozymes Ile55Thr and Asp66His, corresponding to human amyloidogenic mutant lysozymes Ile56Thr and Asp67His, respectively, were secreted in Saccharomyces cerevisiae. The amyloidogenic mutants (I55T and D66H) of hen egg white lysozymes were remarkably less soluble than that of the wild-type pr...

journal_title：FEBS letters

authors： Song Y,Azakami H,Hamasu M,Kato A

更新日期：2001-02-23 00:00:00

abstract：:Bispecific miniantibodies were constructed by genetically fusing the C(H)1 domain of an IgG1 to the C-terminus of a single-chain Fv fragment (scFv-425), specific for the EGF receptor, and fusing the C(L) domain of a kappa light chain to the C-terminus of a scFv specific for CD2 (scFv-M1). An efficient dicistronic gene...

journal_title：FEBS letters

authors： Müller KM,Arndt KM,Strittmatter W,Plückthun A

更新日期：1998-01-30 00:00:00

abstract：:Alternative splicing of the Alzheimer's amyloid beta protein precursor (ABPP) message leads to the production of several variants of this precursor polypeptide. Two of these variants contain a domain that is highly homologous to members of the Kunitz class of protease inhibitors. In order to initiate a study of the ph...

journal_title：FEBS letters

authors： Castro M,Marks CB,Nilsson B,Anderson S

更新日期：1990-07-16 00:00:00

abstract：:Treatment of human neutrophils with triphenyltin chloride (TPTCl)-inhibited superoxide (O-2) production stimulated with phorbol myristate acetate (PMA). TPTCl was more potent as inhibitor of O-2 production than other phenyltin compounds. The O-2 production by the xanthine oxidase-acetaldehyde system was not inhibited ...

journal_title：FEBS letters

authors： Matsui H,Wada O,Ushijima Y,Akuzawa T

更新日期：1983-12-12 00:00:00

abstract：:cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein ...

journal_title：FEBS letters

authors： Merkulova MI,Andreeva SG,Shuvaeva TM,Novoselov SV,Peshenko IV,Bystrova MF,Novoselov VI,Fesenko EE,Lipkin VM

更新日期：1999-04-30 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in ...

journal_title：FEBS letters

authors： Peri KG,MacKenzie RE

更新日期：1991-12-02 00:00:00

abstract：:We focused on the functional involvement of transforming growth factor-beta-activated kinase 1 (TAK1) in transcriptional regulation of interleukin-2 (IL-2) in T cells. Costimulation of Jurkat cells with 12-O-tetradecanoylphorbol-13-acetate and A23187 leads to a rapid phosphorylation of TAK1 and TAK1-binding protein 1 ...

journal_title：FEBS letters

authors： Sakurai H,Singhirunnusorn P,Shimotabira E,Chino A,Suzuki S,Koizumi K,Saiki I

更新日期：2005-12-05 00:00:00

abstract：:Membrane incorporation and aggregation of the peptide alamethicin have been investigated as a function of lipid type. Head group and acyl chain regions both contribute to modulate alamethicin incorporation. Specifically, the peptide prefers thin membranes and saturated chains; incorporation is reduced by the presence ...

journal_title：FEBS letters

authors： Stankowski S,Schwarz G

更新日期：1989-07-03 00:00:00

abstract：:The role of lipids in the interaction of the beta-adrenergic receptor (R) with the regulatory protein (Gs) was investigated. Solubilized preparations of R and of Gs from turkey erythrocytes were delipidated by gel filtration. They were subsequently combined and reconstituted by the addition of various lipids. When rec...

journal_title：FEBS letters

authors： Kirilovsky J,Steiner-Mordoch S,Selinger Z,Schramm M

更新日期：1985-04-08 00:00:00

abstract：:S100, a subfamily of the EF-hand type calcium sensing proteins, is implicated in many cellular functions including muscle contractility. Two isoforms, S100A1 and S100B, at 2-10 microM significantly inhibit active tension, stiffness and ATPase of skinned single rabbit psoas muscle fibers at sub-maximal (pCa approximate...

journal_title：FEBS letters

authors： Adhikari BB,Wang K

更新日期：2001-05-25 00:00:00

abstract：:Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) is common and is associated with a more severe liver disease and increased frequency in the development of hepatocellular carcinoma (HCC). Here, we demonstrated that HBV X protein (HBx) and HCV core protein additively repress the universal cyclin-de...

journal_title：FEBS letters

authors： Han HJ,Jung EY,Lee WJ,Jang KL

更新日期：2002-05-08 00:00:00

abstract：:Vitamin D3, cholecalciferol, induces cell-cell communication via gap junctions in murine fibroblasts (C3H/10T 1/2 cells) at concentrations between 0.01 and 1.0 microM, as assayed by the dye transfer method using Lucifer yellow CH. The extent of induction is similar to that obtained with the positive controls, canthaxa...

journal_title：FEBS letters

authors： Stahl W,Nicolai S,Hanusch M,Sies H

更新日期：1994-09-19 00:00:00

abstract：:The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...

journal_title：FEBS letters

authors： Simões AP,Camps M,Schnabel P,Gierschik P

更新日期：1995-05-29 00:00:00

abstract：:We recently showed that some of the enzymes underpinning cellulose solubilization by Ruminococcus albus 8 lack the conventional type of dockerin module characteristic of cellulosomal proteins and instead, bear an "X" domain of unknown function at their C-termini. We have now subcloned and expressed six X domains and s...

journal_title：FEBS letters

authors： Xu Q,Morrison M,Nelson KE,Bayer EA,Atamna N,Lamed R

更新日期：2004-05-21 00:00:00

abstract：:We have recorded (13)C nuclear magnetic resonance (NMR) spectra of [3-(13)C]Ala, [1-(13)C]Val-labeled pharaonis phoborhodopsin (ppR or sensory rhodopsin II) incorporated into egg PC (phosphatidylcholine) bilayer, by means of site-directed high-resolution solid-state NMR techniques. Seven (13)C NMR signals from transme...

journal_title：FEBS letters

authors： Arakawa T,Shimono K,Yamaguchi S,Tuzi S,Sudo Y,Kamo N,Saitô H

更新日期：2003-02-11 00:00:00

abstract：:Jasmonic acid (JA) has been shown to be a signaling compound which elicits the production of secondary metabolites including phytoalexins in plants. It has been shown that the phytoalexin production is elicited by exogenously applied JA in rice leaves. We now show that this phytoalexin production by exogenously applie...

journal_title：FEBS letters

authors： Tamogami S,Rakwal R,Kodama O

更新日期：1997-07-21 00:00:00

abstract：:Both HIV infection per se and antiretroviral drugs might contribute to oxidative stress and mitochondrial dysfunctions. In this study we assess zidovudine, stavudine and didanosine on U937 and CEM cell lines. All these drugs induced apoptosis and increased intracellular hydrogen peroxide but not superoxide anions. The...

journal_title：FEBS letters

authors： Ferraresi R,Troiano L,Roat E,Nemes E,Lugli E,Nasi M,Pinti M,Calvani M,Iannuccelli M,Cossarizza A

更新日期：2006-12-11 00:00:00

abstract：:The temperature dependence of the rate constants of the univalent redox steps YzoxSi----YzSi + 1 (i = 0,1,2) and YzoxS3----(YzS4)----YzSo + O2 in the water oxidase was investigated by measuring time resolved absorption changes at 355 nm induced by a laser flash train in dark adapted PS II membrane fragments from spina...

journal_title：FEBS letters

authors： Renger G,Hanssum B

更新日期：1992-03-24 00:00:00

abstract：:It has recently been discovered that glutathione-dependent formaldehyde dehydrogenase (FALDH) exhibits a strong S-nitrosoglutathione reductase activity. Plants use NO and S-nitrosothiols as signaling molecules to activate defense mechanisms. Therefore, it is interesting to investigate the regulation of FALDH by mechan...

journal_title：FEBS letters

authors： Díaz M,Achkor H,Titarenko E,Martínez MC

更新日期：2003-05-22 00:00:00

abstract：:By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes ...

journal_title：FEBS letters

authors： Liu N,Delcour AH

更新日期：1998-08-28 00:00:00

abstract：:Recently, we have reported the presence of kinetin (N6-furfuryladenine) in commercially available DNA, in freshly extracted cellular DNA and in plant cell extracts. We have also found that kinetin has electrochemical properties which can be used for monitoring the level of this modified base in DNA. Here, for the firs...

journal_title：FEBS letters

authors： Barciszewski J,Siboska GE,Pedersen BO,Clark BF,Rattan SI

更新日期：1997-09-08 00:00:00

abstract：:The use of tobacco plants as a novel expression system for the production of human homotrimeric collagen I is presented in this report. Constructs were engineered from cDNA encoding the human proalpha1(I) chain to generate transgenic tobacco plants expressing collagen I. The recombinant proalpha1(I) chains were expres...

journal_title：FEBS letters

authors： Ruggiero F,Exposito JY,Bournat P,Gruber V,Perret S,Comte J,Olagnier B,Garrone R,Theisen M

更新日期：2000-03-03 00:00:00

abstract：:Dosage compensation involves fine-tuning of gene expression at the level of entire chromosomes. The principles that assure selective targeting of the male X chromosome in Drosophila and the mechanism by which transcription levels are adjusted in a twofold range are still mysterious. We discuss the prevalent models in ...

journal_title：FEBS letters

authors： Straub T,Dahlsveen IK,Becker PB

更新日期：2005-06-13 00:00:00

abstract：:Eukaryotic porins are a group of membrane proteins whose best known role is to form an aqueous pore channel in the mitochondrial outer membrane. As opposed to the bacterial porins (a large family of protein whose 3D structure has been determined by X-ray diffraction), the structure of eukaryotic porins (also termed VD...

journal_title：FEBS letters

authors： Casadio R,Jacoboni I,Messina A,De Pinto V

更新日期：2002-06-05 00:00:00

abstract：:We examined whether a proposed spatial proximity between Asp114(2.50) and Asn332(7.49) affected the functional properties of the mu opioid receptor. The D114(2.50)N mutant had reduced binding affinities for morphine, DAMGO and CTAP, but not for naloxone and [3H]diprenorphine; this mutation also abolished agonist-induc...

journal_title：FEBS letters

authors： Xu W,Ozdener F,Li JG,Chen C,de Riel JK,Weinstein H,Liu-Chen LY

更新日期：1999-03-26 00:00:00

abstract：:To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...

journal_title：FEBS letters

authors： Su S,Gedamu L,Dehnade F,Zafarullah M

更新日期：1996-10-21 00:00:00

abstract：:The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provid...

journal_title：FEBS letters

authors： Birukov KG,Shirinsky VP,Vorotnikov AV,Gusev NB

更新日期：1990-03-26 00:00:00