Abstract:
:In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å resolution and successfully fitted into our small angle X-ray scattering (SAXS) data of the SHU-complex. Taken together with our electrophoretic mobility shift assay (EMSA) results, a model is proposed for the SHU-protein complex coupled with DNA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
She Z,Gao ZQ,Liu Y,Wang WJ,Liu GF,Shtykova EV,Xu JH,Dong YHdoi
10.1016/j.febslet.2012.06.024subject
Has Abstractpub_date
2012-07-30 00:00:00pages
2306-12issue
16eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00502-9journal_volume
586pub_type
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