Abstract:
:Chronic exposure of a human myeloma cell line to dexamethasone resulted in a selection of cells resistant to the growth-inhibitory action of the glucocorticoid. Upon acute exposure of the parental myeloma cells to dexamethasone growth inhibition was associated with depression of ornithine decarboxylase (ODC, EC 4.1.1.17) activity. However, in cells adapted to grow in the presence of micromolar concentrations of dexamethasone, ODC activity was fully comparable to that in the parental cells. Restriction enzyme analyses with the two isoschizomers HpaII and MspI as well as with the methylation-sensitive CfoI, indicated that the otherwise heavily methylated ODC gene(s) were rendered hypomethylated in the myeloma cells resistant to dexamethasone. This hypomethylation within and/or around ODC genes was associated with a 2-4-fold enhancement of accumulation of ODC mRNA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Leinonen P,Alhonen-Hongisto L,Laine R,Jänne OA,Jänne Jdoi
10.1016/0014-5793(87)80115-1subject
Has Abstractpub_date
1987-05-04 00:00:00pages
68-72issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(87)80115-1journal_volume
215pub_type
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