Transforming growth factor-beta inhibits nephronectin-induced osteoblast differentiation.

abstract：:Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray a...

journal_title：FEBS letters

authors： Hagiwara K,Katsuda T,Gailhouste L,Kosaka N,Ochiya T

更新日期：2015-12-21 00:00:00

abstract：:The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...

journal_title：FEBS letters

authors： Russo R,Borghi R,Markesbery W,Tabaton M,Piccini A

更新日期：2005-11-07 00:00:00

abstract：:In tritium-hydrogen exchange experiments, the large GroEL substrate Rubisco was unfolded and exchanged in urea/acid/tritiated water, then diluted into either protic buffer or protic buffer containing GroEL. The respective Rubisco metastable folding intermediate or Rubisco-GroEL binary complex was then separated from r...

journal_title：FEBS letters

authors： Park ES,Fenton WA,Horwich AL

更新日期：2005-02-14 00:00:00

abstract：:Alterations in protein composition or dosage within chromatin may trigger changes in processes such as gene expression and DNA repair. Through transposon mutagenesis and targeted gene deletions in haploids and diploids of Saccharomyces cerevisiae, we identified mutations that affect telomeric silencing in genes encodi...

journal_title：FEBS letters

authors： Benbow SZ,DuBois ML

更新日期：2008-02-20 00:00:00

abstract：:Peroxynitrite (ONOO-) has recently been implicated in connective tissue destruction in vivo. We have studied the effect of ONOO- on the activity of tissue inhibitor of metalloproteinase-1 (TIMP-1) in vitro. The inactivation of TIMP-1 by ONOO- was dose dependent with 50 microM ONOO- reducing the inhibitory activity of ...

journal_title：FEBS letters

authors： Frears ER,Zhang Z,Blake DR,O'Connell JP,Winyard PG

更新日期：1996-02-26 00:00:00

abstract：:Steady-state kinetic analysis was performed on the reaction between D-fructose and ferricyanide with the quinohemoprotein fructose dehydrogenase from Gluconobacter species. The D-fructose oxidation dependence on the ferricyanide concentration resulted in a series of parallel reciprocal plots, and the reaction was assu...

journal_title：FEBS letters

authors： Marcinkeviciene J,Johansson G

更新日期：1993-02-22 00:00:00

abstract：:Glucose-regulated protein 58 (GRP58)-like immunoreactivity in rat liver obtained in the evening or after fasting underwent an electrophoretic band-shift, which disappeared after phosphatase-treatment. Since mass spectrometric analysis raised a possibility that Ser150 of GRP58 is phosphorylated, an antibody against the...

journal_title：FEBS letters

authors： Kita K,Okumura N,Takao T,Watanabe M,Matsubara T,Nishimura O,Nagai K

更新日期：2006-01-09 00:00:00

abstract：:Holocytochrome c synthase (HCCS) attaches heme covalently to mitochondrial respiratory cytochromes c. Little is known about the reaction of heme attachment to apocytochromes c by HCCS, although recently it has been established that the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important prote...

journal_title：FEBS letters

authors： Zhang Y,Stevens JM,Ferguson SJ

更新日期：2014-09-17 00:00:00

abstract：:Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologo...

journal_title：FEBS letters

authors： Di Berardino M,Dijkstra A,Stüber D,Keck W,Gubler M

更新日期：1996-08-26 00:00:00

abstract：:The Na-Ca exchanger is essential for the Ca2+ homeostasis in many cell types. This transporter has been difficult to investigate because no specific inhibitor is available. We have synthesized an antisense oligodeoxynucleotide directed against the rat cardiac Na-Ca exchanger mRNA. To estimate the activity of the Na-Ca...

journal_title：FEBS letters

authors： Lipp P,Schwaller B,Niggli E

更新日期：1995-05-08 00:00:00

abstract：:The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant bl...

journal_title：FEBS letters

authors： Vauti F,Prochnow BR,Freese E,Ramasamy SK,Ruiz P,Arnold HH

更新日期：2007-12-11 00:00:00

abstract：:Homeodomain-interacting protein kinase 2 (HIPK2) interacts with and phosphorylates various transcription factors that are critical regulators of cell fate decisions and apoptosis during development. Here we show that lysine 25 of HIPK2 is the major sumoylation site, both in vitro and in vivo, and that the sumoylation ...

journal_title：FEBS letters

authors： Sung KS,Go YY,Ahn JH,Kim YH,Kim Y,Choi CY

更新日期：2005-06-06 00:00:00

abstract：:Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

journal_title：FEBS letters

authors： McIntyre GD,Chaudhuri S,Coggins JR

更新日期：1984-10-01 00:00:00

abstract：:To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-...

journal_title：FEBS letters

authors： Zhang G,Guo J,Zhou J,Wang X,Li Q,Yang Y,Shen H,Zhao D,Zhang H,Xi J,Wang L,Qiao S,Jin X

更新日期：2006-02-20 00:00:00

abstract：:On the basis of its predicted homology to human telomerase reverse transcriptase (hTERT), a cDNA for Arabidopsis thaliana TERT (AtTERT) has now been isolated from cultured cells. The cDNA contains an open reading frame of 3372 bp, encoding a protein with a predicted size of 131 kDa and isoelectric point of 9.9. The At...

journal_title：FEBS letters

authors： Oguchi K,Liu H,Tamura K,Takahashi H

更新日期：1999-09-03 00:00:00

abstract：:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...

journal_title：FEBS letters

authors： Hung F,Luo D,Sauvé DM,Muller MT,Roberge M

更新日期：1996-02-12 00:00:00

abstract：:The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...

journal_title：FEBS letters

authors： Geelen MJ,Hindriks GA

更新日期：1984-09-17 00:00:00

abstract：:Cytotoxic effects were observed following 4 h incubation of human leukaemic cells with the iron chelator 1-hydroxypyridine-2-thione (omadine). Its cytotoxic activity was comparable to that of the cytotoxic drug doxorubicin. At the same concentration two other effective iron chelators, desferrioxamine and 1,2-dimethyl-...

journal_title：FEBS letters

authors： Kontoghiorghes GJ,Piga A,Hoffbrand AV

更新日期：1986-08-18 00:00:00

abstract：:Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...

journal_title：FEBS letters

authors： Bartling B,Hilgefort C,Friedrich I,Silber RE,Simm A

更新日期：2003-12-18 00:00:00

abstract：:To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted ami...

journal_title：FEBS letters

authors： Yamazaki N,Shinohara Y,Shima A,Terada H

更新日期：1995-04-17 00:00:00

abstract：:Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...

journal_title：FEBS letters

authors： Chen H,Wu Y,Zhang Y,Jin L,Luo L,Xue B,Lu C,Zhang X,Yin Z

更新日期：2006-05-29 00:00:00

abstract：:The Golgi apparatus is known to modify and sort newly synthesized secretory proteins. However, fundamental mysteries remain about the structure, operation, and dynamics of this organelle. Important insights have emerged from studying the Golgi in yeasts. For example, yeasts have provided direct evidence for Golgi cist...

journal_title：FEBS letters

authors： Papanikou E,Glick BS

更新日期：2009-12-03 00:00:00

abstract：:Mouse epidermal cytosol contains a protein phosphatase with Mr 38,000, which dephosphorylates the elongation factor 2 (EF-2) of protein biosynthesis and is stimulated after topical application of TPA to mouse skin [(1988) Biochem. Biophys. Res. Commun. 153, 1129-1135]. Dephosphorylation of EF-2 by this phosphatase is ...

journal_title：FEBS letters

authors： Gschwendt M,Kittstein W,Mieskes G,Marks F

更新日期：1989-11-06 00:00:00

abstract：:Association reactions of a recombinant CFTR-NBF-2 polypeptide fused to glutathione S-transferase with guanine nucleotides were monitored quantitatively by recording the fluorescence enhancement of excited trinitrophenol (TNP)-labelled GTP after binding to NBF-2. Binding of TNP-GTP to the recombinant NBF-2 polypeptide ...

journal_title：FEBS letters

authors： Randak C,Neth P,Auerswald EA,Assfalg-Machleidt I,Roscher AA,Hadorn HB,Machleidt W

更新日期：1996-11-25 00:00:00

abstract：:A tetradecapeptide with a sequence identical to residues 26-39 of the cro protein from bacteriophage lambda has been synthesized. This peptide has no secondary structure in an aqueous buffer but adopts an alpha-helical conformation in the presence of 20% hexafluoroisopropanol. The fluorescence of the single tyrosyl re...

journal_title：FEBS letters

authors： Mayer R,Lancelot G,Hélène C

更新日期：1983-03-21 00:00:00

abstract：:The uncoupler-induced inactivation of H+-ATPase in hepatoma 22a and mouse liver mitochondria has been studied. The dependence of this process on delta microH, and pH and ATP was established. The inactivated ATPase could be reactivated at alkaline pH values in the absence of ATP. These data indicate that the inactivati...

journal_title：FEBS letters

authors： Chernyak BV,Dukhovich VF,Khodjaev EYu

更新日期：1987-05-11 00:00:00

abstract：:Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentratio...

journal_title：FEBS letters

authors： Borchert TV,Zeelen JP,Schliebs W,Callens M,Minke W,Jaenicke R,Wierenga RK

更新日期：1995-07-03 00:00:00

abstract：:Endoplasmic reticulum stress (ER-stress) is associated with ataxia telangiectasia mutated (ATM) gene. We present here conclusive data showing that ATM blocks ER-stress induced by tunicamycin or ionizing radiation (IR). X-box protein-1 (XBP-1) splicing, GRP78 expression and caspase-12 activation were increased by tunic...

journal_title：FEBS letters

authors： He L,Kim SO,Kwon O,Jeong SJ,Kim MS,Lee HG,Osada H,Jung M,Ahn JS,Kim BY

更新日期：2009-03-04 00:00:00

abstract：:Rat peritoneal mast cells, labelled with [14C]arachidonic acid, released histamine and [14C]arachidonic acid upon the addition of A23187. The release induced by low concentrations of A23187 was suppressed by removal of extracellular Na+ and by addition of the Na+/H+ exchange inhibitor, amiloride. Addition of protein k...

journal_title：FEBS letters

authors： Linnebjerg H,Hansen HS,Jensen B

更新日期：1988-11-21 00:00:00

abstract：:We report a rapid and sensitive colorimetric approach to quantitate the amount of glucose transporters exposed at the surface of intact cells, using L6 muscle cells expressing GLUT4 containing an exofacial myc epitope. Unstimulated cells exposed to the surface 5 fmol GLUT4myc per mg protein. This value increased to 10...

journal_title：FEBS letters

authors： Wang Q,Khayat Z,Kishi K,Ebina Y,Klip A

更新日期：1998-05-08 00:00:00