Abstract:
:The effects of ribosomal protein L5 on the conformation of 5 S rRNA in the 5 S rRNA-protein L5 complex extracted from rat liver ribosomes have been studied by laser Raman spectroscopy. A comparison of the spectra shows small protein-induced conformational changes in the 5 S rRNA, but most of the base-paired regions appear to be present in the complex with protein L5 as well as in the free 5 S rRNA. Furthermore specific interactions between 5 S rRNA and protein L5 are indicated. Cytosine (and/or uracil) residues in single-stranded regions and the N(7) of guanine are engaged in interactions with the protein as suggested by the Raman data.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fabian H,Böhm S,Carius W,Misselwitz R,Welfle Hdoi
10.1016/0014-5793(82)80622-4subject
Has Abstractpub_date
1983-05-08 00:00:00pages
285-90issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(82)80622-4journal_volume
155pub_type
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