Abstract:
:Cytochrome oxidase o has been isolated from the obligately aerobic, methylotrophic bacterium Methylophilus methylotrophus in the form of a cytochrome cL-o complex. The latter is comprised of cytochrome cL (Mr 21 000) and cytochrome o (Mr 29 000) in a 1-2:1 ratio, possibly in association with one or more minor polypeptides; the complex exhibits a high ascorbate-TMPD oxidase activity which is inhibited non-competitively by cyanide (Ki approximately 2 microM). In contrast, the oxidation of methanol by whole cells is inhibited uncompetitively by cyanide (Ki approximately 4 microM), thus indicating the involvement in methanol oxidation of cytochrome oxidase aa3 rather than o.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Carver MA,Jones CWdoi
10.1016/0014-5793(82)80599-1subject
Has Abstractpub_date
1983-05-08 00:00:00pages
187-91issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(82)80599-1journal_volume
155pub_type
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