Abstract:
:Dicarbonyl/L-xylulose reductase (DCXR) converts l-xylulose into xylitol, and reduces various α-dicarbonyl compounds, thus performing a dual role in carbohydrate metabolism and detoxification. In this study, we identified DHS-21 as the only DCXR ortholog in Caenorhabditis elegans. The dhs-21 gene is expressed in various tissues including the intestine, gonadal sheath cells, uterine seam (utse) cells, the spermathecal-uterus (sp-ut) valve and on the plasma membrane of spermatids. Recombinant DHS-21 was shown to convert L-xylulose to xylitol using NADPH as a cofactor. Dhs-21 null mutants of C. elegans show defects in longevity, reproduction and egg-laying. Knock-down of daf-16 and elt-2 transcription factors affected dhs-21 expression. These results suggest that DHS-21 is a bona fide DCXR of C. elegans, essential for normal life span and reproduction.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Son le T,Ko KM,Cho JH,Singaravelu G,Chatterjee I,Choi TW,Song HO,Yu JR,Park BJ,Lee SK,Ahnn Jdoi
10.1016/j.febslet.2011.03.062subject
Has Abstractpub_date
2011-05-06 00:00:00pages
1310-6issue
9eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00228-6journal_volume
585pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Previous data have suggested an involvement of MDR/PGP-like ABC transporters in transport of the plant hormone auxin and, recently, AtPGP1 has been demonstrated to catalyze the primary active export of auxin. Here we show that related isoform AtPGP4 is expressed predominantly during early root development. AtPGP4 loss...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.08.061
更新日期:2005-10-10 00:00:00
abstract::Pheromone receptors are expressed in the accessory olfactory system, which is vital for non-specific chemical communication and for sexual behavior. Under the hypothesis that some of the pheromone molecules released from female reproductive organs might regulate sperm chemotaxis or chemokinesis, we examined whether th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02411-x
更新日期:2001-01-19 00:00:00
abstract::Amino acid sequences of cytotoxin-like basic proteins (CLBPs), purified from the venoms of Formosan cobra (Naja naja atra) and Indian cobra (Naja naja), were reinvestigated. The determined sequences differed from those reported previously by Takechi et al. [(1985) Biochem. Int. 11, 795-802; (1987) Biochem. Int. 14, 14...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81561-3
更新日期:1989-11-06 00:00:00
abstract::Endothelin (ET-1) has been shown to exert vasoconstrictor activity in vivo and mobilize Ca2+ in vascular smooth muscle cells in culture. In this paper we show that the human skin fibroblast exhibits specific receptors to ET-1 and that activation of these receptors results in increased intracellular Ca2+ (Ca2+i) and ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81713-2
更新日期:1989-10-09 00:00:00
abstract::A native female-specific chemoreceptive protein of a swallowtail butterfly [oviposition stimulant binding protein (OSBP)] was shown to specifically bind to aristolochic acid, a main stimulant for oviposition from its host plant. Oviposition stimulants are recognized by chemoreceptive organs of insects. OSBP isolated p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.027
更新日期:2009-01-22 00:00:00
abstract::Light-dependent Na+ and H+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium Oscillatoria brevis. In the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular Na+ level is shown to increase in the dark and d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81268-s
更新日期:1990-09-17 00:00:00
abstract::Under hypobaric hypoxia, antioxidant defenses of the heart are stressed by the enhanced production of ROS. Mammalian heart acclimatizes to hypoxia through altered gene expression, which we studied in murine heart exposed to 10h of acute hypobaric hypoxia (AHH), equivalent to 15000ft, using cDNA arrays. Functional clas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.08.044
更新日期:2007-10-02 00:00:00
abstract::Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80700-2
更新日期:1988-04-11 00:00:00
abstract::In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13279
更新日期:2018-12-01 00:00:00
abstract::Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB), which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses. Here, we investigated roles of transforming growth factor-beta activated kin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01146-7
更新日期:2000-02-11 00:00:00
abstract::Recombinant expression of the large alternansucrase (2057 amino acids) was hindered in E. coli due to poor enzyme solubility and protein degradation. The effects of deletions of the alternansucrase C-terminal CW-like and APY repeated motifs on enzyme solubility and specificity were investigated. A truncated variant de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.001
更新日期:2006-02-06 00:00:00
abstract::Flagellins of two Escherichia coli strains have been investigated by limited proteolysis and scanning microcalorimetry. It has been shown that a monomer flagellin consists of two parts: a central one cooperatively melting, rather resistant to proteases, and the other without a stable tertiary structure and thus easily...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81047-0
更新日期:1988-12-05 00:00:00
abstract::CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00929-6
更新日期:1998-08-21 00:00:00
abstract::We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00035-x
更新日期:1996-02-12 00:00:00
abstract::A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01309-1
更新日期:1995-12-11 00:00:00
abstract::The ARG-11 gene in Saccharomyces cerevisiae encodes a protein with the characteristic features of a family of 35 related membrane proteins that are encoded in the fungal genome. Some of them are known to transport various substrates and products across the inner membranes of mitochondria, but the functions of 29 membe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00630-3
更新日期:1997-06-30 00:00:00
abstract::Using conserved fingerprints in the glycosyltransferase (GTase) domain of high-molecular-weight penicillin-binding proteins (PBP), a gene (mgt) encoding a putative monofunctional glycosyltransferase has been identified in Haemophilus influenzae and in other bacteria] species. Here we report the cloning of the homologo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00809-5
更新日期:1996-08-26 00:00:00
abstract::BIR domain and its containing proteins play critical roles in cell apoptosis and cell division. Here several lines of novelty were revealed based on a comprehensive evolutionary analysis of BIR domains in 11 representative organisms. First, the type II BIR domains in Survivin and Bruce showed more conservation compare...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.09.058
更新日期:2008-11-12 00:00:00
abstract::The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.012
更新日期:2015-10-07 00:00:00
abstract::The solution structure of endothelin-1, a newly discovered potent bicyclic peptide vaso-constrictor agent, has been investigated using 1H NMR conformational constraints and distance geometry calculations. The conformation is constrained by two disulphide bridges between Cys1-Cys15 and Cys3-Cys11 but the NMR data and c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80071-a
更新日期:1991-01-14 00:00:00
abstract::Thioredoxin-interacting protein (Txnip) knockout (TKO) mice exhibit impaired response to fasting. Herein, we showed that activation of adenine monophosphate-activated protein kinase and cellular AMP levels were diminished in the heart and soleus muscle but not in gastrocnemius muscle of fasting TKO mice. Similarly, gl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.03.042
更新日期:2011-04-20 00:00:00
abstract::Curcumin, the yellow pigment from Curcuma longa, has been shown to possess tumoricidal activity. We have earlier reported the induction of apoptosis in AK-5, rat histiocytic cells by curcumin leading to the inhibition of tumor growth in vivo. In this study we have observed differential activation status in host macrop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02089-5
更新日期:2000-10-13 00:00:00
abstract::Mutations in PTEN-induced putative kinase 1 (PINK1) cause recessive form of Parkinson's disease (PD). PINK1 acts upstream of parkin, regulating mitochondrial integrity and functions. Here, we show that PINK1 in combination with parkin results in the perinuclear mitochondrial aggregation followed by their elimination. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.016
更新日期:2010-03-19 00:00:00
abstract::In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(93)81409-s
更新日期:1993-06-28 00:00:00
abstract::We introduced rat ornithine transcarbamylase (OTC) gene into OTC-deficient spf-ash mice by mating spf-ash heterozygotes with transgenic mice which carried recombinant DNA composed of 1.3 kb of the 5' flanking region of the gene fused onto rat OTC cDNA. The liver OTC activity of hemizygous spf-ash mice which carried th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80148-v
更新日期:1991-02-25 00:00:00
abstract::This study reports for the first time (a) the longitudinal profile of the transmembrane potential (mDeltapsi) of single mitochondria using a Nernstian fluorescent probe and (b) the distribution of mDeltapsi fluctuations of mitochondria undergoing permanent depolarization. Our findings show that (1) mitochondria in dif...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01683-5
更新日期:2000-06-23 00:00:00
abstract::Placentas of mice lacking p57(Kip2) expression have trophoblastic hyperplasia. To elucidate the mechanism underlying this phenomenon, we studied expression of two angiogenic factors, vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Immunohistochemical analysis with anti-VEGF antibodies indi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03681-5
更新日期:2002-12-18 00:00:00
abstract::An essential difference between eukaryotic ferritins and bacterioferritins is that the latter contain naturally, in vivo haem as Fe-protoporphyrin IX. This haem is located in a hydrophobic pocket along the 2-fold symmetry axes and is liganded by two Met 52. However, in in vivo studies, a cofactor has been isolated in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.034
更新日期:2006-11-13 00:00:00
abstract::Reconstituted Na+,K+-ATPase from either pig kidney or shark rectal glands was phosphorylated by cAMP dependent protein kinase, PKA. The stoichiometry was approximately 0.9 mol P(i)/mol alpha-subunit in the pig kidney enzyme and approximately 0.2 mol P(i)/mol alpha-subunit in the shark enzyme. In shark, Na+,K+-ATPase P...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00032-4
更新日期:1996-02-19 00:00:00
abstract::Desmoplastic small round cell tumor (DSRCT) is a malignant human cancer that is associated with a specific t(11;22) chromosome translocation, where 265 amino acids from the EWS amino-terminus are fused to the DNA binding domain of the WT1 tumor suppressor gene. We have noticed the presence of several SH3 interacting d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01590-8
更新日期:2000-06-02 00:00:00