Solubilization of angiotensin II receptors in rat glomeruli.

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:A functional NAD molecule was immobilized at the active site of Alcohol dehydrogenase within a proteic membrane. The presence and the functionality of the cofactor was checked by fluorescence analysis. The dehydrogenase NAD membrane does not require addition of soluble cofactor for its activity. The system represents ...

journal_title：FEBS letters

authors： Legoy MD,le Moullec JM,Thomas D

更新日期：1978-10-15 00:00:00

abstract：:Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

journal_title：FEBS letters

authors： Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

更新日期：2018-04-01 00:00:00

abstract：:We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...

journal_title：FEBS letters

authors： Prescott M,Lourbakos A,Bateson M,Boyle G,Nagley P,Devenish RJ

更新日期：1997-07-07 00:00:00

abstract：:Oligopeptides derived from the gag polyprotein (Pr55gag) of human immunodeficiency virus type 1 (HIV-1) segment were used to evaluate the extension of the putative binding region for the complex of Pr55gag and the human cytosolic peptidyl prolyl cis/trans isomerase (PPIase) 18 kDa cyclophilin (Cyp18). Five N-terminall...

journal_title：FEBS letters

authors： Schutkowski M,Drewello M,Wöllner S,Jakob M,Reimer U,Scherer G,Schierhorn A,Fischer G

更新日期：1996-10-07 00:00:00

abstract：:Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...

journal_title：FEBS letters

authors： Demeshkina N,Jenner L,Westhof E,Yusupov M,Yusupova G

更新日期：2013-06-27 00:00:00

abstract：:Leucine-rich repeats (LRRs) are 22-28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide deri...

journal_title：FEBS letters

authors： Gay NJ,Packman LC,Weldon MA,Barna JC

更新日期：1991-10-07 00:00:00

abstract：:Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...

journal_title：FEBS letters

authors： Kooistra T,van Hinsbergh V,Havekes L,Kempen HJ

更新日期：1984-05-07 00:00:00

abstract：:The interaction between two codon-anticodon duplexes of the ribosomal A- and P-site-bound tRNAs is the key feature of the proposed model. This interaction prohibits non-canonical base pairing at the first and second positions of the codon and controls base pairing at the third position (wobbling rules ensuing from the...

journal_title：FEBS letters

authors： Lim VI,Venclovas C

更新日期：1992-11-23 00:00:00

abstract：:A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...

journal_title：FEBS letters

authors： Yamamoto T,Imai M,Tachibana K,Mayumi M

更新日期：1986-01-20 00:00:00

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:Horse heart cytochrome c is cleaved by thermolysin in 50% aqueous TFE (v/v) at neutral pH (25 degrees C, 24 h) at the Gly56-Ile57 peptide bond of the 104-residue chain of the protein. Additional, but anyway minor, fragmentation at the Gly45-Phe46 and Met80-Ile81 peptide bonds is also observed. On the other hand, in bu...

journal_title：FEBS letters

authors： Fontana A,Zambonin M,De Filippis V,Bosco M,Polverino de Laureto P

更新日期：1995-04-10 00:00:00

abstract：:ASP is a serine protease secreted by Aeromonas sobria, a sepsis-causing bacterium, and induces sepsis-mimicking disorders through plasma protein cleavage. The pathogen also secretes nASP that has a nick in the carboxy-terminal region. Compared with single-chain ASP (sASP), nASP had near-equivalent activity for small p...

journal_title：FEBS letters

authors： Murakami Y,Wada Y,Kobayashi H,Hasegawa M,Okamoto K,Eto M,Imamura T

更新日期：2012-10-19 00:00:00

abstract：:As a result of an experimental confluence between carcinogenesis and oxidative phosphorylation, we published in 1971 'A Unitary Hypothesis for Carcinogenesis' [(1971) J. Antibiot. Tokyo 24, 405-417]. According to this hypothesis, damaged mitochondria release mitochondrial genetic material which like that from an oncog...

journal_title：FEBS letters

authors： Hadler HI

更新日期：1989-10-09 00:00:00

abstract：:The promoter of the cholecystokinin (CCK) gene possesses evolutionary conserved juxtaposed E-box and cAMP/TPA responsive elements (CRE/TRE). We have examined the functional interaction of these two sites. As previously noted, c-Jun/c-Fos heterodimers greatly increase promoter activity through association with the CRE/...

journal_title：FEBS letters

authors： Rourke IJ,Hansen TV,Nerlov C,Rehfeld JF,Nielsen FC

更新日期：1999-04-01 00:00:00

abstract：:The freshwater ciliate Tetrahymena pyriformis is an ubiquitous organism that is present in all aquatic ecosystems. This protozoan showed a clear resistance against some polycyclic aromatic hydrocarbons which can be attributed to an efflux pump probably of the multidrug resistance (MDR) type. Immunocytochemical detecti...

journal_title：FEBS letters

authors： Bamdad M,Brousseau P,Denizeau F

更新日期：1999-08-13 00:00:00

abstract：:We present evidence that the potent chloride channel blocker NPPB has protonophoric activity in the mitochondria and across the plasma membrane of phagocytic cells. The resting O2 consumption of murine peritoneal macrophages was stimulated up to 2.5-fold in the presence of NPPB, with a K0.5 of 15 microM. The stimulato...

journal_title：FEBS letters

authors： Lukacs GL,Nanda A,Rotstein OD,Grinstein S

更新日期：1991-08-19 00:00:00

abstract：:In the presence of pyrithione, which was used as a Zn2+ ionophore, Zn2+ (10-100 microM) increased phorbol ester binding by intact B-CLL cells in a dose-dependent fashion. Zn pyrithione increased 2-fold the number of phorbol ester receptors in B-cells (0.74 to 1.4 pmol/10(6) cells), neutrophil polymorphs (0.2 to 0.51 p...

journal_title：FEBS letters

authors： Forbes IJ,Zalewski PD,Hurst NP,Giannakis C,Whitehouse MW

更新日期：1989-04-24 00:00:00

abstract：:A chemokine receptor, CXCR4, and its endogenous ligand, stromal cell-derived factor-1 (SDF-1), have been recognized to be involved in the metastasis of several types of cancers. T140 analogs are peptidic CXCR4 antagonists composed of 14 amino acid residues that were previously developed as anti-HIV agents having inhib...

journal_title：FEBS letters

authors： Tamamura H,Hori A,Kanzaki N,Hiramatsu K,Mizumoto M,Nakashima H,Yamamoto N,Otaka A,Fujii N

更新日期：2003-08-28 00:00:00

abstract：:In C4 plants the activity of phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is regulated by phosphorylation/dephosphorylation which is mediated by light/dark signals. The study using protein kinase inhibitors showed that the inhibition pattern of maize PEPC-protein kinase (PEPC-PK) is similar to that of myosin li...

journal_title：FEBS letters

authors： Ogawa N,Okumura S,Izui K

更新日期：1992-05-04 00:00:00

abstract：:For understanding the mechanism(s) relating inflammation to corticosteroid action, the effect of tumour necrosis factor-alpha (TNF-alpha) on 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2), the enzyme regulating access of 11beta-hydroxycorticosteroids to receptors, was studied in LLC-PK(1) cells. We observed ...

journal_title：FEBS letters

authors： Heiniger CD,Rochat MK,Frey FJ,Frey BM

更新日期：2001-11-02 00:00:00

abstract：:Aminopeptidase yscXVI was purified from the yeast Saccharomyces cerevisiae. By SDS-PAGE the enzyme has a molecular weight of 45,000 Da, and in chromatofocusing, elution was observed at pH 6.2. The synthetic substrate cystinyl-4-nitroanilide (Km 22.5 microM, Vmax 12.9 mU/mg) is cleaved most efficiently in the pH range ...

journal_title：FEBS letters

authors： Tisljar U,Wolf DH

更新日期：1993-05-10 00:00:00

abstract：:A bark lectin, RBL, from Robinia pseudoacacia (black locust), binds galactose-related sugars specifically. Recombinant RBL (rRBL) with a histidine tag was expressed in Escherichia coli, purified and characterized. rRBL agglutinated rabbit erythrocytes and the hemagglutination was inhibited by galactose and related sug...

journal_title：FEBS letters

authors： Nishiguchi M,Yoshida K,Sumizono T,Tazaki K

更新日期：1997-02-24 00:00:00

abstract：:In several reactions catalyzed by highly purified peptide elongation factor 1 from rabbit reticulocytes, GTP may be fully replaced by CTP but not by ATP or UTP. This holds true for the factor-dependent binding of aminoacyl-tRNA to ribosomes, GTPase activity, GTP-dependent autophosphorylation of the factor protein and ...

journal_title：FEBS letters

authors： Tuhácková Z,Havránek M,Hradec J

更新日期：1984-11-05 00:00:00

abstract：:Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are current...

journal_title：FEBS letters

authors： Shin DH,Choi M,Kim K,Bang G,Cho M,Choi SB,Choi G,Park YI

更新日期：2013-05-21 00:00:00

abstract：:Bovine alpha 2-antiplasmin (alpha 2AP) has been purified and partially characterized. The amino acid composition is very similar to that of human alpha 2AP, and the N-terminal (23 residues determined) and reactive site loop sequences (42 residues determined) are highly homologous to those of the human protein. Compare...

journal_title：FEBS letters

authors： Christensen S,Sottrup-Jensen L

更新日期：1992-11-02 00:00:00

abstract：:Zymosan, which is composed primarily of alpha-mannan and beta-glucan polymers, is a well recognized activator of macrophages. The type receptor by which unopsonized zymosan induces arachidonic acid release was investigated. It was found that particulate beta-glucan and zymosan stimulated an identical dose-dependent re...

journal_title：FEBS letters

authors： Daum T,Rohrbach MS

更新日期：1992-09-07 00:00:00

abstract：:Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functio...

journal_title：FEBS letters

authors： Krause K,Kilbienski I,Mulisch M,Rödiger A,Schäfer A,Krupinska K

更新日期：2005-07-04 00:00:00

abstract：:The expression of the cytochrome P4501A1 gene, CYP1A1, is induced by e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) mainly by transcriptional mechanisms. The inducers mediate their effect upon binding and activation of the aryl hydrocarbon receptor (AHR) transcription-factor complex. Utilizing chimeric CYP1A1/CAT con...

journal_title：FEBS letters

authors： Jørgensen EC,Autrup H

更新日期：1995-05-29 00:00:00

abstract：:Suppression of electron flow (and its subsequent restoration with 3-10 mM NaHCO3) on the donor side of photosystem II is shown upon either a partial depletion of pea subchloroplast membranes in bicarbonate or the addition of 5-20 microM formate. At higher concentrations (5 mM) formate induces the known 'bicarbonate ef...

journal_title：FEBS letters

authors： Klimov VV,Allakhverdiev SI,Feyziev YaM,Baranov SV

更新日期：1995-04-24 00:00:00