Abstract:
:The expression of the cytochrome P4501A1 gene, CYP1A1, is induced by e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) mainly by transcriptional mechanisms. The inducers mediate their effect upon binding and activation of the aryl hydrocarbon receptor (AHR) transcription-factor complex. Utilizing chimeric CYP1A1/CAT constructs transient gene expression experiments indicate that the putative negative regulatory element (NRE) of CYP1A1 influence the relative TCDD induced CAT activity in HepG2 cells, whereas this effect was not observed in MCF-7 cells. Differences in the formation of cell-specific protein-DNA complexes were demonstrated by gel retardation assays suggesting a functional difference of NRE in these two cell lines.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jørgensen EC,Autrup Hdoi
10.1016/0014-5793(95)00456-jsubject
Has Abstractpub_date
1995-05-29 00:00:00pages
101-7issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00456-Jjournal_volume
365pub_type
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