Abstract:
:Recent studies indicate that junctophilin (JP) subtypes contribute to the formation of the junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum (ER/SR) in excitable cells. Cardiac muscle contains the diad, in which the transverse (T) tubule of the invaginated cell membrane is closely associated with the SR membrane, and skeletal muscle bears the triad, in which the T-tubule is associated with two SR membranes on the both sides. Among defined JP subtypes, JP-2 is specifically expressed in cardiac muscle, while skeletal muscle cells contain both JP-1 and JP-2. These observations, together with other findings, suggest that the triad might be constructed in a JP-1-dependent manner after the achievement of JP-2-mediated diad formation during skeletal muscle maturation. In this study using transgenic mice, we examined whether the triad can be formed when JP-1 is additionally expressed in cardiac muscle. Immunochemical analysis demonstrated co-expression of JP-1 and JP-2 in cardiac myocytes from the transgenic mice. In cardiac muscle expressing JP-1, abnormal junctional membranes were frequently observed under the electron microscope, in which the T-tubules were rolled up with the SR membranes at several turns, but authentic triad formation could not be detected. Therefore, ectopic JP-1 expression cannot convert the diad to the triad in cardiac myocytes. The present results suggest that triad formation requires an as yet unknown skeletal muscle-specific mechanism, in addition to the JP subtypes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Komazaki S,Nishi M,Takeshima Hdoi
10.1016/s0014-5793(03)00340-5keywords:
subject
Has Abstractpub_date
2003-05-08 00:00:00pages
69-73issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303003405journal_volume
542pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.077
更新日期:2007-09-04 00:00:00
abstract::Several of the recently described leucocyte surface (glyco)-proteins with significant amino acid sequence similarity (human CD9, CD37, CD53, CD63, TAPA-1, CO-029 and R2 and several homologues of other species) are distinguished by the polypeptide chain apparently four times crossing the membrane. Although the biologic...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(91)80988-f
更新日期:1991-08-19 00:00:00
abstract::The polymorphism at the multitude of loci adjacent to human endogenous retrovirus long terminal repeats (LTRs) was analyzed by a technique for whole genome differential display based on the PCR suppression effect that provides selective amplification and display of genomic sequences flanking interspersed repeated elem...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00004-6
更新日期:1999-01-29 00:00:00
abstract::Differentiation of glia (astrocytes and oligodendrocytes) in Drosophila requires the gene glial cells missing (gcm), which controls lineage determination. In the absence of gcm, neuroglia progenitors exclusively differentiate into neurons, instead of into both neurons and glia. In contrast, ectopic overexpression of g...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00890-3
更新日期:1996-09-16 00:00:00
abstract::We show here that the genome of F9 teratocarcinoma cells growing in culture is heavily methylated but undergoes massive demethylation in tumors derived by subcutaneous injection of the cells. This demethylation occurs primarily in single copy gene sequences. As a result imprinted genes acquire their characteristic mon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00994-5
更新日期:1996-10-14 00:00:00
abstract::Glycogen storage diseases type 1 (GSD 1) are a group of autosomal recessive disorders characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Mutations of the glucose-6-phosphatase gene are responsible for the most frequent form of GSD 1, the subtype 1a, while mutations of the glucose-6-ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01248-x
更新日期:1999-10-08 00:00:00
abstract::We have expressed green fluorescent protein (GFP) from A. victoria in the secretory pathway of HeLa cells by fusing it to the C-terminus of a secretory protein, chromogranin B. Under normal culture conditions at 37 degrees C maturation of GFP to the fluorescent form was not detectable. However, fluorescent GFP was obs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00765-2
更新日期:1995-08-07 00:00:00
abstract::Shiga toxin-producing Escherichia coli (STEC) is associated with hemolytic uremic syndrome (HUS). Although most clinical isolates of STEC produce hemolysin (called enterohemolysin), the precise role of enterohemolysin in the pathogenesis of STEC infections is unknown. Here we demonstrated that E. coli carrying the clo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03027-2
更新日期:2002-07-31 00:00:00
abstract::The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.07.038
更新日期:2015-09-14 00:00:00
abstract::Hell's Gate globin I (HGbI), a heme-containing protein structurally homologous to mammalian neuroglobins, has been identified from an acidophilic and thermophilic obligate methanotroph, Methylacidiphilum infernorum. HGbI has very high affinity for O(2) and shows barely detectable autoxidation in the pH range of 5.2-8....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.002
更新日期:2011-10-20 00:00:00
abstract::Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12716
更新日期:2017-08-01 00:00:00
abstract::Deuterated arachidonic acid (AA, [2H8]20:4 n-6) 1-25 microM, is converted to other fatty acids, as evaluated by gas chromatography-mass spectrometry, in THP-1 cells. The major products, in the 1 to 10 microM range, are 22:4 (elongated) and 20:3 (reduced in 5). At 25 microM, 24:4, 24:5 and 22:5 accumulate, with [2H8]/[...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80554-7
更新日期:1994-05-02 00:00:00
abstract::Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH-3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00945-6
更新日期:1995-09-18 00:00:00
abstract::The L1CAM antibody A10-A3 efficiently reduces tumor growth in a nude mouse model. Here, we describe the crystal structure of the Fab fragment of A10-A3 determined at 2.0 angstrom resolution. The A10-A3 antibody H3 loop reveals a characteristic arrangement of exposed aromatic residues that may play an important role in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.028
更新日期:2011-01-03 00:00:00
abstract::Phosphorylation of H2AX is believed to be associated with the repair of damaged DNA. Recent findings suggest a novel function of H2AX in cellular apoptosis. Specifically, it was shown that ultraviolet A-activated JNK phosphorylates H2AX to regulate apoptosis. Here we show that serum starvation induces H2AX phosphoryla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.051
更新日期:2008-08-06 00:00:00
abstract::Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03072-1
更新日期:2001-11-23 00:00:00
abstract::Human T-cell leukemia and bovine leukemia viruses have a potential transforming gene, termed X. In addition to the major open reading frame known to encode a functional protein, the X gene harbors another short open reading frame which overlaps this major one. Both of these open reading frames are found on a single sp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80038-7
更新日期:1985-11-11 00:00:00
abstract::The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.054
更新日期:2004-06-04 00:00:00
abstract::The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02528-5
更新日期:2001-06-15 00:00:00
abstract::The retinal pigment epithelium-specific 65 kDa (RPE65) isomerase plays a pivotal role in photoreceptor survival and function. RPE65-catalyzed synthesis of 11-cis-retinol from all-trans-retinyl esters in the visual cycle is negatively regulated, through a heretofore unknown mechanism, by the fatty acid transport protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13633
更新日期:2020-02-01 00:00:00
abstract::OmpC, a surface antigen of Salmonella typhi was crystallized after several attempts, using PEG 3350. Well shaped hexagonal crystals were grown from vapor diffusion method using octyl glucoside and C12E9 as detergents. Crystals are sensitive to X-ray and diffract weakly up to 7 A. Porin isoforms, due to the bound lipop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00746-2
更新日期:1999-06-25 00:00:00
abstract::MiR-140 is a microRNA specially involved in chondrogenesis and osteoarthritis pathogenesis. However, its transcriptional regulation and target genes in cartilage development are not fully understood. Here we detected that miR-140 was uniquely expressed in chondrocyte and suppressed by Wnt/β-catenin signalling. The miR...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.013
更新日期:2011-10-03 00:00:00
abstract::Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an int...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12547
更新日期:2017-01-01 00:00:00
abstract::Guluronate and mannuronate oligomers with various degree of polymerization were prepared from polyguluronate (PG) and polymannuronate (PM) with an alginate lyase from a Pseudoalteromonas sp., and their activities to induce cytokine secretion from mouse macrophage cell line RAW264.7 cells were examined. Enzymatically d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.07.007
更新日期:2005-08-15 00:00:00
abstract::Leonor Michaelis spent the years of 1922-1926 as Professor of Biochemistry of the Aichi Medical College (now Graduate School of Medicine, Nagoya University) in Nagoya, Japan. Michaelis succeeded in gathering many bright young biochemists from all over Japan into his laboratory, and made tremendous contributions to the...
journal_title:FEBS letters
pub_type: 传,历史文章,杂志文章
doi:10.1016/j.febslet.2013.04.020
更新日期:2013-09-02 00:00:00
abstract::A truncated human Na(+)/glucose cotransporter (C(5), residues 407-664) was expressed and purified from Escherichia coli using a GST fusion vector and glutathione affinity chromatography. The truncated transporter (C(5)) was cleaved from GST-C(5) by Factor Xa proteolysis and purified by gel filtration chromatography. U...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01292-2
更新日期:1999-10-15 00:00:00
abstract::Both HIV infection per se and antiretroviral drugs might contribute to oxidative stress and mitochondrial dysfunctions. In this study we assess zidovudine, stavudine and didanosine on U937 and CEM cell lines. All these drugs induced apoptosis and increased intracellular hydrogen peroxide but not superoxide anions. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.016
更新日期:2006-12-11 00:00:00
abstract::The whole-cell patch-clamp technique was used to measure cell membrane capacitance (Cm) to monitor exocytosis in single-cultured bovine prolactin-secreting cells (lactotrophs) of the anterior pituitary. The cells were dialyzed with solutions containing different concentrations of ionised Ca and non-hydrolyzable GTP an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80936-6
更新日期:1989-08-14 00:00:00
abstract::A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80044-h
更新日期:1992-02-24 00:00:00
abstract::A new secondary structure model for parts of the 7 SL RNA is proposed which indicates for a stretch of at least 40 bases a strong structural homology to the ribosomal protein L5 binding site of eukaryotic 5 S rRNA. It is suggested that the 5 S rRNA-like structural part of 7 SL RNA mediates binding of the signal recogn...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81548-x
更新日期:1987-02-09 00:00:00