Abstract:
:Cytochrome bd is one of the two terminal ubiquinol oxidases in the respiratory chain of Escherichia coli catalyzing reduction of O2 to H2O. The enzyme is expressed under low oxygen tension; due to high affinity for O2 it is isolated mainly as a stable oxygenated complex. Direct measurement of O2 binding to heme d in the one-electron reduced isolated enzyme gives K(d(O2)) of approximately 280 nM. It is possible to photolyse the heme d oxy-complex by illumination of the enzyme for several minutes under microaerobic conditions; the light-induced difference absorption spectrum is virtually identical to the inverted spectrum of O2 binding to heme d.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Belevich I,Borisov VB,Konstantinov AA,Verkhovsky MIdoi
10.1016/j.febslet.2005.07.011keywords:
subject
Has Abstractpub_date
2005-08-29 00:00:00pages
4567-70issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00854-9journal_volume
579pub_type
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