Abstract:
:A single chain ribosome-inactivating protein with RNA N-glycosidase activity, here named Dianthin 29, was isolated from leaves of Dianthus barbatus L. Incubation of intact Escherichia coli ribosomes with Dianthin 29 and subsequent aniline treatment of the isolated rRNA releases a rRNA fragment of 243 nucleotides from 23 S rRNA. Nucleotide sequence studies showed that the site of N-glycosidic bond cleavage is at A-2660 within the universally conserved sequence 5'-AGUACGAGAGGA-3' near the 3'-end of 23/28 S rRNAs. To our knowledge, Dianthin 29 is the first ribosome-inactivating protein which is shown to inactivate intact prokaryotic ribosomes in the same manner as eukaryotic ribosomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Prestle J,Hornung E,Schönfelder M,Mundry KWdoi
10.1016/0014-5793(92)80549-vkeywords:
subject
Has Abstractpub_date
1992-02-10 00:00:00pages
250-2issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(92)80549-Vjournal_volume
297pub_type
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