12-O-tetradecanoyl phorbol-13-acetate interferes with germination of Phycomyces blakesleeanus sporangiospores.


:The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by 6 nM TPA. The TPA analog 4 alpha PDD, an ineffective activator of PKC, did not affect spore germination. 3 mM dbcAMP, on the other hand, reversed the inhibition of germination caused by TPA. TPA-stimulated protein kinase activity was detected in spores. The possible relationship between PKC and the increased levels of cAMP that accompany the induction of spore germination is discussed.




FEBS letters


Carrillo-Rayas T,Garcia-Soto J,Martinez-Cadena G




Has Abstract


1988-10-10 00:00:00














  • Hsa-miR-125b suppresses bladder cancer development by down-regulating oncogene SIRT7 and oncogenic long non-coding RNA MALAT1.

    abstract::MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/oncogenic long non-coding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic down-regulated, whereas hsa-miR-125b inhibitor up-regulated the expression of SIRT7 and MALA...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Han Y,Liu Y,Zhang H,Wang T,Diao R,Jiang Z,Gui Y,Cai Z

    更新日期:2013-11-29 00:00:00

  • Complement component C8gamma is expressed in human fetal and adult kidney independent of C8alpha.

    abstract::Human complement component C8gamma is an unusual complement factor since it shows no homology to other complement proteins but is a member of the lipocalin superfamily. So far, it has been found exclusively in plasma, covalently linked to C8alpha by disulfide bridging. We have used dot blot and Northern blot analyses ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Trojer P,Wojnar P,Merschak P,Redl B

    更新日期:1999-03-12 00:00:00

  • Linkage between phosphorylated cystatin alpha and filaggrin by epidermal transglutaminase as a model of cornified envelope and inhibition of cathepsin L activity by cornified envelope and the conjugated cystatin alpha.

    abstract::Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi M,Tezuka T,Kakegawa H,Katunuma N

    更新日期:1994-03-07 00:00:00

  • Mitochondrial uncoupling proteins and phylogenesis--UCP4 as the ancestral uncoupling protein.

    abstract::We searched for the previously defined uncoupling protein (UCP) signatures [Jezek, P. and Urbánková, E. (2000) IUBMB Life 49, 63-70] in genomes of Drosophila melanogaster, Caenorhabditis elegans, Dictyostelium discoideum, and Arabidopsis thaliana. We identified four UCPs in Drosophila and one in Caenorhabditis or Dict...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hanák P,Jezek P

    更新日期:2001-04-27 00:00:00

  • 8-Chloro-dGTP, a hypochlorous acid-modified nucleotide, is hydrolyzed by hMTH1, the human MutT homolog.

    abstract::The human mutT homolog, hMTH1, suppresses spontaneous mutations by degrading the endogeneous mutagen, 8-hydroxy-dGTP. We previously reported the broad substrate specificity of hMTH1, which also degrades the oxidatively damaged purine nucleotides, 2-hydroxy-dATP, 8-hydroxy-dATP, 2-hydroxy-ATP, and 8-hydroxy-GTP, in add...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fujikawa K,Yakushiji H,Nakabeppu Y,Suzuki T,Masuda M,Ohshima H,Kasai H

    更新日期:2002-02-13 00:00:00

  • Differential expression of AMPA glutamate receptor mRNAs in the rat adrenal gland.

    abstract::Localisation of mRNA encoding the four AMPA glutamate receptor subunits in the rat adrenal gland was investigated using in situ hybridisation. GluR1 is found in the zona glomerulosa of the cortex, GluR3 in the remaining parts of the cortex, GluR2 in adrenal medullary cells and GluR4 at a very low level in the zona glo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kristensen P

    更新日期:1993-10-11 00:00:00

  • Ganglioside headgroups decrease lipid order in reconstituted phosphatidylcholine liposomes.

    abstract::The effect of oligosaccharide carrying lipids on membrane fluidity has been investigated. Gangliosides GM1 and GQ1 were reconstituted into phosphatidylcholine bilayer membranes at low concentrations (less than 5 mol%). A strong fluidizing effect was observed leading to a suppression of the phase transition temperature...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ollmann M,Galla HJ

    更新日期:1985-01-01 00:00:00

  • A novel family of carbohydrate-binding modules identified with Ruminococcus albus proteins.

    abstract::We recently showed that some of the enzymes underpinning cellulose solubilization by Ruminococcus albus 8 lack the conventional type of dockerin module characteristic of cellulosomal proteins and instead, bear an "X" domain of unknown function at their C-termini. We have now subcloned and expressed six X domains and s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Xu Q,Morrison M,Nelson KE,Bayer EA,Atamna N,Lamed R

    更新日期:2004-05-21 00:00:00

  • A D-antifreeze polypeptide displays the same activity as its natural L-enantiomer.

    abstract::The D- and L-forms of an alpha-helical antifreeze polypeptide (AFP) have been chemically synthesized. Circular dichroism spectra of the molecules show equal and opposite ellipticites. The D- and the L-enantiomers alone, and a 50:50 mixture of the two, all show identical antifreeze activity, but the enantiomeric forms ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wen D,Laursen RA

    更新日期:1993-02-08 00:00:00

  • Phosphoinositide hydrolysis in permeabilized SH-SY5Y human neuroblastoma cells is inhibited by mastoparan.

    abstract::The effects of mastoparan on phospholipase C-catalysed phosphoinositide hydrolysis were examined in [3H]inositol-labelled human neuroblastoma SH-SY5Y cells. [3H]Inositol phosphate formation in intact cells was not altered by 20 microM mastoparan. In contrast, [3H]inositol phosphate formation in electrically permeabili...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wojcikiewicz RJ,Nahorski SR

    更新日期:1989-04-24 00:00:00

  • PDT-induced HSP70 externalization up-regulates NO production via TLR2 signal pathway in macrophages.

    abstract::We studied the molecular mechanism underlying PDT-induced apoptosis-dependent macrophage activation, particularly through NO production. We demonstrate that NO production is initially induced by HSP70 on the apoptotic cell surface, and is further enhanced by macrophage phagocytosis. Additionally, we found that apoptot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Song S,Zhou F,Chen WR,Xing D

    更新日期:2013-01-16 00:00:00

  • FHA domain boundaries of the dun1p and rad53p cell cycle checkpoint kinases.

    abstract::Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

    更新日期:2000-04-14 00:00:00

  • Hypoxia-inducible factor-1alpha regulates matrix metalloproteinase-1 activity in human bone marrow-derived mesenchymal stem cells.

    abstract::We examined the mRNA levels of hypoxia-inducible factor-1alpha (HIF-1alpha) in bone marrow mesenchymal stem cells (bmMSCs) of eight osteoarthritis patients. BmMSC-1, expressing higher HIF-1alpha mRNA and protein than bmMSC-5, elicited higher matrix metalloproteinase-1 (MMP1) activity and stronger invasive capacity. In...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lin JL,Wang MJ,Lee D,Liang CC,Lin S

    更新日期:2008-07-23 00:00:00

  • Rescue of a neutralizing anti-viral antibody fragment from an intracellular polyclonal repertoire expressed in mammalian cells.

    abstract::The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this rea...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gargano N,Cattaneo A

    更新日期:1997-09-15 00:00:00

  • N-3 PUFAs modulate global gene expression profile in cultured rat cardiomyocytes. Implications in cardiac hypertrophy and heart failure.

    abstract::In cardiac cells the effects of n-3 PUFAs on the whole genome are still unknown despite their recognized cardioprotective effects and ability to modulate gene expression. We have evaluated the effects of n-3 PUFAs supplementation on the global gene expression profile in cultured neonatal rat cardiomyocytes, detecting ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bordoni A,Astolfi A,Morandi L,Pession A,Danesi F,Di Nunzio M,Franzoni M,Biagi P,Pession A

    更新日期:2007-03-06 00:00:00

  • Competitive binding of the troponin T-specific pool of caldesmon antibodies and tropomyosin to skeletal troponin T and smooth muscle caldesmon.

    abstract::The fraction of polyclonal caldesmon antibodies cross-reacting with rabbit skeletal troponin T are shown to compete with smooth muscle tropomyosin for caldesmon and troponin T, as revealed by ELISA method. The epitope recognized by these antibodies was also found in Mr 77 kDa non-muscle caldesmon. These results provid...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Birukov KG,Shirinsky VP,Vorotnikov AV,Gusev NB

    更新日期:1990-03-26 00:00:00

  • Evidence for a role of glycosphingolipids in CXCR4-dependent cell migration.

    abstract::Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Limatola C,Massa V,Lauro C,Catalano M,Giovanetti A,Nuccitelli S,Spinedi A

    更新日期:2007-06-12 00:00:00

  • Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-β-glucosidases.

    abstract::Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SG

    更新日期:2016-02-01 00:00:00

  • Meproadifen enhances activation and desensitization of the acetylcholine receptor-ionic channel complex (AChR): single channel studies.

    abstract::The effects of the quaternary agent meproadifen on ACh-activated channel currents were studied on myoballs cultured from hind limb muscles of neonatal rats. Meproadifen (0.02-0.1 microM) combined with ACh (0.1-0.3 microM) in the patch pipette caused an increase, followed by a decrease, in the frequency of channel open...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Aracava Y,Albuquerque EX

    更新日期:1984-09-03 00:00:00

  • Overlapping CRE and E-box promoter elements can independently regulate COX-2 gene transcription in macrophages.

    abstract::Macrophage cyclooxygenase-2 (COX-2) transcription is mediated through the collaboration of different promoter elements. Here, the role of an overlapping cyclic AMP responsive element (CRE)/E-box was investigated. Nuclear proteins bound both the CRE and E-box, which synergized with other promoter elements to induce COX...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mestre JR,Rivadeneira DE,Mackrell PJ,Duff M,Stapleton PP,Mack-Strong V,Maddali S,Smyth GP,Tanabe T,Daly JM

    更新日期:2001-05-11 00:00:00

  • Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem-resistant host.

    abstract::Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoneyama H,Nakae T

    更新日期:1991-06-03 00:00:00

  • Polyunsaturated fatty acids potentiate interleukin-1-stimulated arachidonic acid release by cells overexpressing type IIA secretory phospholipase A2.

    abstract::By analyzing human embryonic kidney 293 cell transfectants stably overexpressing various types of phospholipase A2 (PLA2), we have shown that polyunsaturated fatty acids (PUFAs) preferentially activate type IIA secretory PLA2 (sPLA2-IIA)-mediated arachidonic acid (AA) release from interleukin-1 (IL-1)-stimulated cells...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kambe T,Murakami M,Kudo I

    更新日期:1999-06-18 00:00:00

  • An acid amidase hydrolyzing anandamide as an endogenous ligand for cannabinoid receptors.

    abstract::Anandamide loses its cannabimimetic activities upon hydrolysis to arachidonic acid and ethanolamine. So far the anandamide hydrolyzing activity widely distributed in mammalian organs has been attributed exclusively to an enzyme referred to as anandamide amidohydrolase with an optimum pH around 9. We found another enzy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ueda N,Yamanaka K,Terasawa Y,Yamamoto S

    更新日期:1999-07-09 00:00:00

  • The membrane insertion of colicins.

    abstract::Pore-forming toxins, such as colicin A, are water-soluble proteins that insert into lipid bilayers. The water-soluble structure of Colicin A is known at a high resolution and this review describes the kinetic and structural steps involved in its soluble-to-membrane bound transformation. ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Lakey JH,González-Mañas JM,van der Goot FG,Pattus F

    更新日期:1992-07-27 00:00:00

  • Quantitative structure activity relationships for the conversion of nitrobenzimidazolones and nitrobenzimidazoles by DT-diaphorase: implications for the kinetic mechanism.

    abstract::Quantitative structure activity relationships (QSARs) for the conversion of nitrobenzimidazolones and nitrobenzimidazoles by rat liver DT-diaphorase (EC are described. The parameter used for description of the QSARs is the energy of the lowest unoccupied molecular orbital (E(LUMO)) of the nitroaromatic compo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Anusevicius Z,Soffers AE,Cénas N,Sarlauskas J,Segura-Aguilar J,Rietjens IM

    更新日期:1998-05-15 00:00:00

  • The crystal structure of triacylglycerol lipase from Pseudomonas glumae reveals a partially redundant catalytic aspartate.

    abstract::The family of lipases (triacylglycerol-acyl-hydrolases EC constitutes an interesting class of enzymes because of their ability to interact with lipid-water interfaces, their wide range of substrate specificities, and their potential industrial applications. Here we report the first crystal structure of a bact...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Noble ME,Cleasby A,Johnson LN,Egmond MR,Frenken LG

    更新日期:1993-09-27 00:00:00

  • Revised assignments for the beta-, gamma- and delta-subunits of the acetylcholine receptor structural model.

    abstract::Revised assignments for the bilayer helices of the beta-, gamma- and delta-subunits of the acetylcholine receptor are presented. A new feature of the model is extensive charge matching between the polar groups of the ion channel elements of different subunits. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kosower EM

    更新日期:1984-06-25 00:00:00

  • Engineering plant resistance by constructing chimeric receptors that recognize damage-associated molecular patterns (DAMPs).

    abstract::An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: De Lorenzo G,Brutus A,Savatin DV,Sicilia F,Cervone F

    更新日期:2011-06-06 00:00:00

  • The nucleosome remodeling factor.

    abstract::An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Alkhatib SG,Landry JW

    更新日期:2011-10-20 00:00:00

  • Between the ground- and M-state of bacteriorhodopsin the retinal transition dipole moment tilts out of the plane of the membrane by only 3 degrees.

    abstract::The orientation of the transition dipole moments in the ground state and the M-intermediate of bacteriorhodopsin were determined by time-resolved and steady-state polarized absorption spectroscopy on samples of oriented immobilized purple membranes. The angle between the transition dipole moment and the membrane norma...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Otto H,Heyn MP

    更新日期:1991-11-18 00:00:00