12-O-tetradecanoyl phorbol-13-acetate interferes with germination of Phycomyces blakesleeanus sporangiospores.

abstract：:Abstract Type V adenylyl cyclase (AC) was stably over-expressed in HEK293 cells (293AC-V). Forskolin-stimulated cAMP accumulation in 293AC-V was 5 times as great as that in control cells. PMA, a protein kinase C (PKC) activator, enhanced cAMP accumulation in 293AC-V cells dose-and time-dependently and this enhancement...

journal_title：FEBS letters

authors： Kawabe Ji,Ebina T,Toya Y,Oka N,Schwencke C,Duzic E,Ishikawa Y

更新日期：1996-04-22 00:00:00

abstract：:Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

journal_title：FEBS letters

authors： Swain J,Gutteridge JM

更新日期：1995-07-24 00:00:00

abstract：:We identified a yeast mutant with temperature-sensitive growth defects that were rescued by VCP expression. The mutation occurred in GPI10, which encodes a mannosyl transferase for glycosylphosphatidylinositol anchor formation in the endoplasmic reticulum, and caused a Gly469Glu substitution in Gpi10. The mutant exhib...

journal_title：FEBS letters

authors： Ohnuma Y,Takata T,Kawawaki J,Yasuda K,Tanaka K,Kimura Y,Kakizuka A

更新日期：2015-02-27 00:00:00

abstract：:The regulatory mechanism by which GATA-3 suppresses IFN-gamma gene expression was investigated. A reduction of GATA-3 using RNA interference technology enhanced, whereas overexpression of GATA-3 suppressed IFN-gamma mRNA expression. IL-4 expression was reciprocally affected by GATA-3. GATA-3-mediated down-regulation o...

journal_title：FEBS letters

authors： Kaminuma O,Kitamura F,Kitamura N,Miyagishi M,Taira K,Yamamoto K,Miura O,Miyatake S

更新日期：2004-07-16 00:00:00

abstract：:The adipose-derived plasma protein, adiponectin (APN), has various protective effects on cardiovascular diseases. In this study, we show that endogenous APN is required for full cyclooxygenase-2 (COX-2) induction by ischemia-reperfusion injury in the heart in vivo. In rat neonatal cardiac myocytes, APN-induced COX-2 e...

journal_title：FEBS letters

authors： Ikeda Y,Ohashi K,Shibata R,Pimentel DR,Kihara S,Ouchi N,Walsh K

更新日期：2008-04-02 00:00:00

abstract：:Hexaheme nitrite reductases purified to homogeneity from Escherichia coli K-12 and Wolinella succinogenes were studied by low-temperature EPR spectroscopy. In their isolated states, the two enzymes revealed nearly identical EPR spectra when measured at 12 K. Both high-spin and low-spin ferric heme EPR resonances with ...

journal_title：FEBS letters

authors： Liu MC,Liu MY,Payne WJ,Peck HD Jr,Le Gall J,DerVartanian DV

更新日期：1987-06-29 00:00:00

abstract：:The gene of ecotin, an E. coli proteinase inhibitor, was cloned, and by site-directed mutagenesis the active site residue of the protein, Met84, was mutated to Lys, Arg and Leu. The recombinant wild-type and mutant inhibitors were overexpressed in E. coli, purified to homogeneity and their inhibitory effects on trypsi...

journal_title：FEBS letters

authors： Pál G,Sprengel G,Patthy A,Gráf L

更新日期：1994-03-28 00:00:00

abstract：:Based on the amino acid alignment, Caenorhabditis elegans F32D1.1 was identified to be a homologue of the mammalian fidgetin. We produced and purified the F32D1.1 protein by using a baculovirus-expression system. F32D1.1 has an ATPase activity, which is sensitive to N-ethylmaleimide. Km and Vmax for the ATPase activit...

journal_title：FEBS letters

authors： Yakushiji Y,Yamanaka K,Ogura T

更新日期：2004-12-03 00:00:00

abstract：:A simple method for isolation and fluorescence labelling of Leydig cells (L-cells) from rat testes was developed. Lateral diffusion coefficients of both lipid and protein membrane fluorescent probes were measured by the method of fluorescence recovery after photobleaching (FRAP). Age-dependent changes in diffusibility...

journal_title：FEBS letters

authors： Zlatanov IV,Foley M,Birmingham J,Garland PB

更新日期：1987-09-28 00:00:00

abstract：:The gene spa P (formerly designated as spa P1) encoding the Mr 185,000 surface antigen (I/II) of Streptococcus mutans, serotype c (NG5), has been sequenced. The gene (4683 bp) encodes a protein of 1561 amino acid residues including putative signal peptide (residues 1-38) and transmembrane (residues 1537-1556) sequence...

journal_title：FEBS letters

authors： Kelly C,Evans P,Bergmeier L,Lee SF,Progulske-Fox A,Harris AC,Aitken A,Bleiweis AS,Lehner T

更新日期：1989-11-20 00:00:00

abstract：:Intracellular vesicular transport involves targeting of vesicles to their correct destination and subsequent fusion with the target membrane. These processes employ unique compartment-specific proteins in combination with general components which act at several transport steps. This review will summarize recent progre...

journal_title：FEBS letters

authors： Söllner T

更新日期：1995-08-01 00:00:00

abstract：:Hypoxic-ischaemic encephalopathy, resulting from asphyxia during birth, affects 2-3 in every 1000 term infants and depending on severity, brings about life-changing neurological consequences or death. This hypoxic-ischaemia (HI) results in a delayed neural energy failure during which the majority of brain injury occur...

journal_title：FEBS letters

authors： Thornton C,Jones A,Nair S,Aabdien A,Mallard C,Hagberg H

更新日期：2018-03-01 00:00:00

abstract：:We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

journal_title：FEBS letters

authors： Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

更新日期：1999-05-07 00:00:00

abstract：:This study investigates to what extent the expression of the slow myosin heavy chain (MyHCI) isoform and the slow type sarcoplasmic reticulum Ca2+ ATPase (SERCA2a) isoform are co-regulated in fibers of regenerating skeletal soleus muscle. Both overexpression of cain, a calcineurin inhibitor, or partial tenotomy preven...

journal_title：FEBS letters

authors： Zádor E,Fenyvesi R,Wuytack F

更新日期：2005-01-31 00:00:00

abstract：:Matrix metalloproteinase-13 (MMP-13) plays a critical role in parathyroid hormone (PTH)-induced bone resorption. PTH acts via protein kinase A (PKA) to phosphorylate and stimulate the transactivation of Runx2 for MMP-13 promoter activation. We show here that PTH stimulated Runx2 phosphorylation in rat osteoblastic cel...

journal_title：FEBS letters

authors： Selvamurugan N,Shimizu E,Lee M,Liu T,Li H,Partridge NC

更新日期：2009-04-02 00:00:00

abstract：:The influence of poly (ADP-ribose) polymerase (PARP) and poly ADP-ribosylation on DNA synthesis supported by human replicative DNA polymerase (DNA pol) alpha, delta, and epsilon has been examined using the replication system containing poly(dA)4500-oligo(dT)12-18 as the template primer. PARP alone inhibited the pol ac...

journal_title：FEBS letters

authors： Eki T

更新日期：1994-12-19 00:00:00

abstract：:Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771...

journal_title：FEBS letters

authors： Pei ZD,Williamson JR

更新日期：1998-02-13 00:00:00

abstract：:The nuclear factor NF-IL6 had been suggested to be responsible for the IL-6-mediated induction of several acute-phase proteins. To obtain evidence for the involvement of NF-IL6 in the induction of acute-phase proteins, we introduced the NF-IL6 gene and its truncated mutant (delNFIL6) gene into a hepatoma cell line Hep...

journal_title：FEBS letters

authors： Natsuka S,Isshiki H,Akira S,Kishimoto T

更新日期：1991-10-07 00:00:00

abstract：:Spermatid-specific thioredoxin-1 (Sptrx-1) is the first member of the thioredoxin family of proteins with a tissue-specific expression pattern, found exclusively in the tail of elongating spermatids and spermatozoa. We describe here further biochemical characterization of human Sptrx-1 protein structure and enzymatic ...

journal_title：FEBS letters

authors： Jiménez A,Johansson C,Ljung J,Sagemark J,Berndt KD,Ren B,Tibbelin G,Ladenstein R,Kieselbach T,Holmgren A,Gustafsson JA,Miranda-Vizuete A

更新日期：2002-10-23 00:00:00

abstract：:PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...

journal_title：FEBS letters

authors： Philipp S,Flockerzi V

更新日期：1997-08-18 00:00:00

abstract：:Three clones were isolated from a lobster digestive gland cDNA library, using oligonucleotide probes based on the partial amino terminal sequence of a digestive cysteine proteinase. The cDNAs, LCP1, LCP2 and LCP3 encode preproenzymes of 322, 323 and 321 amino acid residues, and putative mature enzymes of 217, 216 and ...

journal_title：FEBS letters

authors： Laycock MV,MacKay RM,Di Fruscio M,Gallant JW

更新日期：1991-11-04 00:00:00

abstract：:Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 mi...

journal_title：FEBS letters

authors： Zhang SQ,Outlaw WH Jr,Chollet R

更新日期：1994-09-19 00:00:00

abstract：:The ability of the Escherichia coli single-stranded DNA-binding protein (SSB) to recognize structural features associated with intramolecular triplex formation in oligopurine.oligopyrimidine (pur.pyr) inserts in recombinant plasmids was evaluated. The SSB protein binds to supercoiled plasmids and causes a site-prefere...

journal_title：FEBS letters

authors： Klysik J,Shimizu M

更新日期：1993-11-01 00:00:00

abstract：:The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

journal_title：FEBS letters

authors： Marais RM,Nguyen O,Woodgett JR,Parker PJ

更新日期：1990-12-17 00:00:00

abstract：:We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-b...

journal_title：FEBS letters

authors： Kase R,Kitagawa H,Hayashi K,Tanoue K,Inagaki F

更新日期：1989-08-28 00:00:00

abstract：:We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery...

journal_title：FEBS letters

authors： Hasan MR,Jin M,Matsushima K,Miyamoto S,Kotani S,Nakagawa H

更新日期：2006-06-12 00:00:00

abstract：:It is shown that conformational changes of receptor proteins brought about by binding of a ligand induce changes in the lipid environment of the receptor that can be monitored by fluorescent lipid probes. On this basis a new approach to studies of ligand-receptor binding is proposed. Using the interaction of the ricin...

journal_title：FEBS letters

authors： Manevich EM,Tonevitsky AG,Bergelson LD

更新日期：1986-01-06 00:00:00

abstract：:Cytosolic phospholipase A(2)alpha (cPLA(2)alpha) plays an important role in arachidonate pathway. To investigate the contribution of cPLA(2)alpha to autoimmune diabetes, we established non-obese diabetic (NOD) mouse, an excellent model for human type 1 diabetes, deficient in cPLA(2)alpha. These mice showed severe insu...

journal_title：FEBS letters

authors： Oikawa Y,Yamato E,Tashiro F,Yamamoto M,Uozumi N,Shimada A,Shimizu T,Miyazaki J

更新日期：2005-07-18 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

journal_title：FEBS letters

authors： Manjithaya R,Nazarko TY,Farré JC,Subramani S

更新日期：2010-04-02 00:00:00