Revisiting the odorant-binding protein LUSH of Drosophila melanogaster: evidence for odour recognition and discrimination.

abstract：:In higher eukaryotes the hypermodified guanine analogue, queuine (7-(5-[(1S,4S,5R)-4,5-dihydroxy-2-cyclopentene-1-yl) amino)-methyl)-7-deazaguanine), occurs free or as modified nucleoside (Q) in the anticodon of specific tRNAs. Fast proliferating tissues and tumors contain considerable amounts of free queuine and Q-de...

journal_title：FEBS letters

authors： Langgut W,Kersten H

更新日期：1990-06-04 00:00:00

abstract：:Disulfide bond formation is a critical step in the folding of many secretory proteins. In bacteria, disulfide bonds are introduced by the periplasmic dithiol oxidase DsbA, which transfers its catalytic disulfide bond to folding polypeptides. Reduced DsbA is reoxidized by ubiquinone Q8, catalyzed by inner membrane quin...

journal_title：FEBS letters

authors： Malojcić G,Owen RL,Grimshaw JP,Glockshuber R

更新日期：2008-10-15 00:00:00

abstract：:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...

journal_title：FEBS letters

authors： Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi H

更新日期：1992-10-05 00:00:00

abstract：:The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha-monomeric CK-II (CK-IIalpha) as well as oligomeric CK-II (alpha2beta2) in a dose-dependent manner, but has no...

journal_title：FEBS letters

authors： Karino A,Tanoue S,Fukuda M,Nakamura T,Ohtsuki K

更新日期：1996-12-02 00:00:00

abstract：:A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...

journal_title：FEBS letters

authors： Guignard L,Ozawa K,Pursglove SE,Otting G,Dixon NE

更新日期：2002-07-31 00:00:00

abstract：:Spin-trapping techniques and electron spin resonance (ESR) spectroscopy were used to study the relationship between the effect of streptozotocin (STZ) on pancreatic beta-cells and free radical formation by these cells. Results showed that STZ enhanced generation of the DMPO-OH radical adduct, which is a degradation pr...

journal_title：FEBS letters

authors： Nukatsuka M,Sakurai H,Yoshimura Y,Nishida M,Kawada J

更新日期：1988-11-07 00:00:00

abstract：:The rat chromosomal gene for uricase was cloned. The gene spans more than 20 kilobases and the coding region is divided into 8 exons by 7 introns. The organization of the rat uricase gene is so greatly different from that of the soybean gene that the difference may not have been caused only by the removal of some ance...

journal_title：FEBS letters

authors： Motojima K,Goto S

更新日期：1990-05-07 00:00:00

abstract：:Urate oxidase from Aspergillus flavus is a 135 kDa homo-tetramer which has a hydrophobic cavity buried within each monomer and located close to its active site. Crystallographic studies under moderate gas pressure and high hydrostatic pressure have shown that both gas presence and high pressure would rigidify the cavi...

journal_title：FEBS letters

authors： Colloc'h N,Prangé T

更新日期：2014-05-02 00:00:00

abstract：:Jasmonic acid (JA) has been shown to be a signaling compound which elicits the production of secondary metabolites including phytoalexins in plants. It has been shown that the phytoalexin production is elicited by exogenously applied JA in rice leaves. We now show that this phytoalexin production by exogenously applie...

journal_title：FEBS letters

authors： Tamogami S,Rakwal R,Kodama O

更新日期：1997-07-21 00:00:00

abstract：:Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in...

journal_title：FEBS letters

authors： Paumi CM,Pickin KA,Jarrar R,Herren CK,Cowley ST

更新日期：2012-03-23 00:00:00

abstract：:The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...

journal_title：FEBS letters

authors： Gowran A,Murphy CE,Campbell VA

更新日期：2009-11-03 00:00:00

abstract：:The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...

journal_title：FEBS letters

authors： van Gastel M,Bubacco L,Groenen EJ,Vijgenboom E,Canters GW

更新日期：2000-06-02 00:00:00

abstract：:We propose a unified framework for the identification of functional interaction structures of biomolecular networks in a way that leads to a new experimental design procedure. In developing our approach, we have built upon previous work. Thus we begin by pointing out some of the restrictions associated with existing s...

journal_title：FEBS letters

authors： Cho KH,Choo SM,Wellstead P,Wolkenhauer O

更新日期：2005-08-15 00:00:00

abstract：:The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD...

journal_title：FEBS letters

authors： Collados R,Andreu V,Picorel R,Alfonso M

更新日期：2006-09-04 00:00:00

abstract：:Incubation of HER14 cells with phorbol myristate acetate (PMA) decreases epidermal growth factor (EGF)-dependent protein tyrosine phosphorylation, except for a 40-kDa MAP kinase II-like protein, whose tyrosine phosphorylation is further enhanced. The inhibitory effect of PMA on EGF-dependent protein tyrosine phosphory...

journal_title：FEBS letters

authors： Errasfa M,Stern A

更新日期：1994-02-14 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Rev is a small RNA-binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N- ...

journal_title：FEBS letters

authors： Jensen TH,Jensen A,Szilvay AM,Kjems J

更新日期：1997-09-01 00:00:00

abstract：:Using a radioimmunoassay for the peptide sequence Arg-Phe-NH2 (RFamide), a novel peptide has been purified from acetic acid extracts of the sea anemone Anthopleura elegantissima. This peptide has the structure less than Glu-Gly-Leu-Arg-Trp-NH2, and was named Antho-RWamide II. Antho-RWamide II is a neuropeptide. Its st...

journal_title：FEBS letters

authors： Graff D,Grimmelikhuijzen CJ

更新日期：1988-10-24 00:00:00

abstract：:Insulin stimulated protein kinase B alpha (PKB alpha) more than 10-fold and decreased glycogen synthase kinase-3 (GSK3) activity by 50 +/- 10% in skeletal muscle and adipocytes. Rapamycin did not prevent the activation of PKB, inhibition of GSK3 or stimulation of glycogen synthase up to 5 min. Thus rapamycin-insensiti...

journal_title：FEBS letters

authors： Cross DA,Watt PW,Shaw M,van der Kaay J,Downes CP,Holder JC,Cohen P

更新日期：1997-04-07 00:00:00

abstract：:Cell stimulation raised the cytosolic free Ca2+ concentration, [Ca(2+]i and induces activation of Na+/H+ exchange which raises the cytosolic pH, pHi. Recent studies have addressed the question whether Na+/H+ exchange plays a role in Ca(2+) influx and, specifically, whether a rise in pHi alone suffices to open Ca(2+) c...

journal_title：FEBS letters

authors： Siffert W,Akkerman JW

更新日期：1989-12-18 00:00:00

abstract：:Prohormone convertases (PCs) 1 and 2 are thought to mediate the proteolytic cleavage of many peptide precursors. Endogenous inhibitors of both PC1 and PC2 have now been identified; the 7B2 protein is a nanomolar inhibitor of PC2, while the novel protein proSAAS was recently reported to be a micromolar inhibitor of PC1...

journal_title：FEBS letters

authors： Cameron A,Fortenberry Y,Lindberg I

更新日期：2000-05-12 00:00:00

abstract：:ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...

journal_title：FEBS letters

authors： Jones CP,Khan K,Ingram-Smith C

更新日期：2017-02-01 00:00:00

abstract：:The angiotensin II type I (AT(1)) receptor mediates regulation of blood pressure and water-electrolyte balance by Ang II. Substitution of Gly for Asn(111) of the AT(1) receptor constitutively activates the receptor leading to Gq-coupled IP(3) production independent of Ang II binding. The Ang II-activated conformation ...

journal_title：FEBS letters

authors： Lee C,Hwang SA,Jang SH,Chung HS,Bhat MB,Karnik SS

更新日期：2007-05-29 00:00:00

abstract：:LIMD1 is a tumour suppressor gene (TSG) down regulated in ∼80% of lung cancers with loss also demonstrated in breast and head and neck squamous cell carcinomas. LIMD1 is also a candidate TSG in childhood acute lymphoblastic leukaemia. Mechanistically, LIMD1 interacts with pRB, repressing E2F-driven transcription as we...

journal_title：FEBS letters

authors： Foxler DE,James V,Shelton SJ,Vallim TQ,Shaw PE,Sharp TV

更新日期：2011-04-06 00:00:00

abstract：:The amplification of hTERT was detected in glioma tissues, although telomerase activity was not always found within these specimens. The aim of this study was to correlate the level of hTERT transcription with telomerase activity in two glioma age groups. hTERT was significantly transcribed at similar copy numbers in ...

journal_title：FEBS letters

authors： Shervington A,Patel R

更新日期：2008-05-28 00:00:00

abstract：:Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...

journal_title：FEBS letters

authors： Hamada M,Kuwabara T,Warashina M,Nakayama A,Taira K

更新日期：1999-11-12 00:00:00

abstract：:Protein kinase CK2 is composed of two regulatory beta-subunits and two catalytic alpha- or alpha'-subunits. To analyse these subunits individually we generated antibodies against unique peptides derived from the alpha-, alpha'- and beta-subunit. Immunofluorescence studies with these antibodies revealed the presence of...

journal_title：FEBS letters

authors： Faust M,Schuster N,Montenarh M

更新日期：1999-11-26 00:00:00

abstract：:The dependence of CMP-N-acetylneuraminate:GM1 sialyltransferase (SAT IV) activity of rat liver Golgi apparatus on GM1 ganglioside ceramide composition was evaluated. SAT IV activity was assayed on GM1 molecular species carrying homogeneous ceramide moieties containing long chain bases of different length (18 or 20 C a...

journal_title：FEBS letters

authors： Pitto M,Palestini P,Masserini M

更新日期：1996-04-01 00:00:00

abstract：:Phospholipase C (PLC) was purified from squid retina. Soluble Gq alpha, membrane Gq alpha and G beta gamma were isolated from GTP gamma S-treated and light-illuminated photoreceptor membranes. The membrane Gq alpha stimulated phosphatidyl inositol-phospholipase C (PI-PLC) activity in a dose-dependent manner. Soluble G...

journal_title：FEBS letters

authors： Suzuki T,Terakita A,Narita K,Nagai K,Tsukahara Y,Kito Y

更新日期：1995-12-27 00:00:00

abstract：:Proteasomes were purified from human hepatoma tissues, and their sensitivities to Na+ and K+ were examined. At concentrations of 10 mM or more, these cations were found to inhibit completely polylysine-activated casein degradation by the purified proteasomes. They also strongly inhibited the hydrolyses of peptides, al...

journal_title：FEBS letters

authors： Seol JH,Park SC,Ha DB,Chung CH,Tanaka K,Ichihara A

更新日期：1989-04-24 00:00:00

abstract：:This study describes for the first time the amphiphilicity of the protein moiety of proteoglycogen. Glycogenin but not proteoglycogen associates to phospholipid vesicles and forms by itself stable Gibbs and Langmuir monolayers at the air-buffer interface. The adsorption free energy (-6.7 kcal/mol) and the glycogenin c...

journal_title：FEBS letters

authors： Carrizo ME,Miozzo MC,Maggio B,Curtino JA

更新日期：2001-12-07 00:00:00