Abstract:
:We previously showed that heat stress stimulates reactive oxygen species (ROS) production in skeletal muscle mitochondria of birds, probably via an elevation in mitochondrial membrane potential (DeltaPsi). To clarify the mechanism underlying the elevation of DeltaPsi, modular kinetic analysis was applied to oxidative phosphorylation in skeletal muscle mitochondria of heat-stressed birds (34 degrees C for 12h). In the birds exposed to heat stress, 'substrate oxidation' (a DeltaPsi-producer) was increased compared to control (24 degrees C) birds, although there was little difference in 'proton leak' (a DeltaPsi-consumer), suggesting that an elevation in the DeltaPsi at state 4 may be due to enhanced substrate oxidation. It thus appears that enhanced substrate oxidation plays a crucial role in the overproduction of ROS for heat-stressed birds, probably via elevated DeltaPsi.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kikusato M,Ramsey JJ,Amo T,Toyomizu Mdoi
10.1016/j.febslet.2010.05.057subject
Has Abstractpub_date
2010-07-16 00:00:00pages
3143-8issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(10)00461-8journal_volume
584pub_type
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