Abstract:
:The contributions of membrane type-1 matrix metalloproteinase (MT1-MMP) and of the glucose-6-phosphate transporter (G6PT) in sphingosine-1-phosphate (S1P)-mediated Ca(2+) mobilization were assessed in glioblastoma cells. We show that gene silencing of MT1-MMP or G6PT decreased the extent of S1P-induced Ca(2+) mobilization, chemotaxis, and extracellular signal-related kinase phosphorylation. Chlorogenic acid and (-)-epigallocatechin-3-gallate, two diet-derived inhibitors of G6PT and of MT1-MMP, respectively, reduced S1P-mediated Ca(2+) mobilization. An intact MT1-MMP/G6PT signaling axis is thus required for efficient Ca(2+) mobilization in response to bioactive lipids such as S1P. Targeted inhibition of either MT1-MMP or G6PT may lead to reduced infiltrative and invasive properties of brain tumor cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fortier S,Labelle D,Sina A,Moreau R,Annabi Bdoi
10.1016/j.febslet.2008.01.061subject
Has Abstractpub_date
2008-03-05 00:00:00pages
799-804issue
5eissn
0014-5793issn
1873-3468pii
S0014-5793(08)00101-4journal_volume
582pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We have used a diethylpyrocarbonate (DEPC) modification [(1976) Prog. Nucl. Acids Res. 16, 189-262] to probe the accessibility of adenines essential for coat protein binding in the MS2 translational operator [(1983) Biochemistry 22, 2601-2610, 2610-2615, 4723-4730; (1987) Biochemistry 26, 1563-1568]. The essential ade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80576-o
更新日期:1991-05-20 00:00:00
abstract::When cultured porcine aortic endothelial cells (ECs) were incubated with porcine big endothelin-1 (bit ET-1(1-39)), there was a time-dependent increase in immunoreactive (IR)-ET in the culture supernatant, in addition to an endogenous IR-ET release fron the cells. Reverse-phase HPLC of the culture supernatant revealed...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81149-3
更新日期:1991-11-18 00:00:00
abstract::Acute myeloid leukemia (AML) associated translocations often cause gene fusions that encode oncofusion proteins. Although many of the breakpoints involved in chromosomal translocations have been cloned, in most cases the role of the chimeric proteins in tumorigenesis is not elucidated. Here we will discuss the fusion ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.04.002
更新日期:2010-06-18 00:00:00
abstract::Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.055
更新日期:2004-10-22 00:00:00
abstract::(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O(2) levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.07.065
更新日期:2012-09-21 00:00:00
abstract::Tropomodulin, the P-end (slow-growing end)-capping protein of the actin-tropomyosin filament, and its fragment (C20) of the C-terminal half were studied by synchrotron small-angle X-ray scattering, restoring low-resolution shapes using an ab initio shape-determining procedure. Tropomodulin is elongated (115 A long) an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02498-x
更新日期:2001-06-01 00:00:00
abstract::The multiline signal of the S(2) state in Photosystem II was measured both in frozen-solution and single-crystal preparations from the cyanobacterium Thermosynechococcus elongatus. The frozen-solution EPR spectrum shows a gaussian-like line shape without any resolution of Mn hyperfine couplings. This line shape can be...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.039
更新日期:2006-06-26 00:00:00
abstract::Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histoc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81037-8
更新日期:1988-12-05 00:00:00
abstract::The DNA binding characteristics of a mono-, di- and trimeric derivative of 9-aminoacridine were studied. The length of the linking carboxamidoalkyl chains was selected to allow bis- or tris-intercalation according to the excluded-site model. Measurements of DNA unwinding angle using closed circular DNA showed that the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80302-6
更新日期:1984-04-24 00:00:00
abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01365-8
更新日期:1995-01-03 00:00:00
abstract::A novel antimicrobial peptide, named misgurin, was isolated and characterized from the loach (mudfish), Misgurnus anguillicaudatus. The 21-amino-acid peptide with a molecular mass of 2502 Da was purified to homogeneity using a heparin-affinity column and C18 reverse-phase and gel-permeation high-performance liquid chr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00684-4
更新日期:1997-07-14 00:00:00
abstract::Recombinant vaccinia virus vectors were used to coexpress each of the candidate prohormone convertases PC1, PC2, furin, PACE4 and PC5 with rat prosomatostatin (rProSOM) in the constitutive secreting cell line LoVo and in the endocrine corticotroph cell line AtT-20, which exhibits regulated secretion. Mammalian ProSOM ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00229-3
更新日期:1995-04-03 00:00:00
abstract::Criteria for the design of peptide vaccines to prevent AIDS are presented. The best vaccine candidates contain both B and T lymphocyte-defined epitopes in regions conserved in sequence between viral isolates. We propose that attention should focus on proteins specified by the gag and, possibly, pol genes in addition t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81052-9
更新日期:1987-06-29 00:00:00
abstract::A 33-kDa protein component of the oxygen-evolving complex in photosystem II is essential for photosynthesis, and it has been believed that mutants with deletion of this 33-kDa protein are not found in higher plants. We report here the first isolation of an Arabidopsis thaliana mutant with a defect in one of the genes ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02963-0
更新日期:2002-07-17 00:00:00
abstract::The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00662-8
更新日期:2003-07-17 00:00:00
abstract::The morphology of complexes between relaxed circular plasmid pBR322 DNA and tripeptide L-Val-L-Val-L-Val-NH-NH-Dns (TVP) at different peptide/DNA ratios was studied by electron microscopy. The results show that interaction of TVP with circular DNA leads to the formation of perfect torus-shaped particles. The torus par...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80236-2
更新日期:1985-01-21 00:00:00
abstract::Caloric restriction (CR) is the most compelling example of lifespan extension by external manipulation. Although the molecular mechanisms remain unknown, the theory of hormesis has been invoked to explain the life promoting effects of CR. Hormesis is defined as the beneficial effects of low intensity stressor on a cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.017
更新日期:2005-12-19 00:00:00
abstract::Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02228-6
更新日期:2000-12-01 00:00:00
abstract::A cDNA coding for a new subunit of soluble guanylyl cyclase with a calculated molecular mass of 81.7 kDa was cloned and sequenced. On the basis of sequence homology, the new subunit appears to be an isoform of the alpha 1-subunit and was designated alpha 2 as the new subunit is very similar to the alpha 1-subunit in t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80871-y
更新日期:1991-11-04 00:00:00
abstract::Cytochrome c oxidase has been isolated from beef heart mitochondria and labeled with the fluorochrome eosin-5-maleimide (EMA) after pretreatment with mersalyl. On SDS-polyacrylamide gels, EMA fluorescence and absorption occurred at a single band corresponding to subunit III. Since only Cys 115 of the two cysteinyl res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80664-5
更新日期:1985-05-06 00:00:00
abstract::Botulinum neurotoxins (BoNTs), the causative agent of human botulism, are the most potent naturally occurring toxins known. BoNT/A1, the most studied BoNT, is also used as an important biopharmaceutical. In this study, the biological activity of BoNT/A1 is compared to that of BoNT/A2 using neuronal cell models. The da...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.11.045
更新日期:2011-01-03 00:00:00
abstract::The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80350-7
更新日期:1986-03-03 00:00:00
abstract::Proteins containing the caspase recruiting domain (CARD) have emerged as critical regulators of different signal transduction pathways, including those controlling apoptosis and activation of necrosis factor (NF)-kappaB transcription factor. TUCAN/CARDINAL is a recently identified CARD-containing protein involved in r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02869-7
更新日期:2002-06-19 00:00:00
abstract::Phalloidin and fluorescently labeled phalloidin analogs are established reagents to stabilize and mark actin filaments for the investigation of acto-myosin interactions. In the present study, we employed transient and steady-state kinetic measurements as well as in vitro motility assays to show that phalloidin perturb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.01.042
更新日期:2011-03-09 00:00:00
abstract::Phosphorylation of H2AX is believed to be associated with the repair of damaged DNA. Recent findings suggest a novel function of H2AX in cellular apoptosis. Specifically, it was shown that ultraviolet A-activated JNK phosphorylates H2AX to regulate apoptosis. Here we show that serum starvation induces H2AX phosphoryla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.051
更新日期:2008-08-06 00:00:00
abstract::Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be requi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13312
更新日期:2019-02-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) cascades play an important role in mediating stress responses in plants. In Arabidopsis, 20 MAPKs have been identified and classified into four major groups (A-D). Little is known about the role of group C MAPKs. We have studied the activation of Arabidopsis subgroup C1 MAPKs (A...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.03.075
更新日期:2007-05-01 00:00:00
abstract::A drastic inhibition of protein synthesis occurs in HeLa cells treated with human lymphoblastoid interferon and infected with poliovirus. At the time when this inhibition has been established no degradation of 32P-labelled ribosomal RNA can be detected. Isolation of the mRNAs from poliovirus-infected cells plus or min...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80942-9
更新日期:1983-08-22 00:00:00
abstract::A new enzyme catalyzing the deamidation of seed storage proteins was found in germinating wheat grains and was partially purified. It also acts on egg lysozyme, horse hemoglobin and reduced RNAse, glutamine and Gly-L-Gln-L-Tyr. No activity was observed when using ovalbumin, serum albumin, RNAse, insulin, asparagine an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80432-g
更新日期:1992-05-11 00:00:00
abstract::Both cisplatin and the estrogen receptor (ER) are known to bend DNA. The influence of the bending of sequences by the d(GpG)cisPt adduct binding of ER to estrogen response element (ERE)-like sequences was examined. Three ERE-like oligonucleotides with different affinities for ER and which include a GG in the linker se...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01755-x
更新日期:2000-01-21 00:00:00