Molecular mechanism of inhibition of mammalian protein synthesis by some four-chain agglutinins. Proposal of an extended classification of plant ribosome-inactivating proteins (rRNA N-glycosidases).


:The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they do not affect plant protein synthesis under the same conditions. Therefore, they should also be considered as true RIPs but of a new class, the four-chain RIPs. An extended classification of RIPs is presented based on the former one from Stirpe et al. [Bio/technology 10 (1992) 405-412].




FEBS letters


Citores L,Ferreras JM,Iglesias R,Carbajales ML,Arias FJ,Jiménez P,Rojo MA,Girbés T




Has Abstract


1993-08-23 00:00:00














  • Inhibition of human telomerase by oligonucleotide chimeras, composed of an antisense moiety and a chemically modified homo-oligonucleotide.

    abstract::Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

    更新日期:2005-02-28 00:00:00

  • Spectroscopic characterization of PS I core complexes from thermophilic Synechococcus sp. Identical reoxidation kinetics of A1- before and after removal of the iron-sulfur-clusters FA and FB.

    abstract::Monomeric and trimeric PS I complexes missing the three stromal subunits E,C and D (termed PS I core complexes) were prepared from the thermophilic cyanobacterium Synechococcus sp. by incubation with urea. The subunits E,C and D are sequentially removed. In the monomeric PS I the subunit C is removed with a half life ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lüneberg J,Fromme P,Jekow P,Schlodder E

    更新日期:1994-01-31 00:00:00

  • Dimeric structure of H(+)-translocating pyrophosphatase from pumpkin vacuolar membranes.

    abstract::Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato MH,Maeshima M,Ohsumi Y,Yoshida M

    更新日期:1991-09-23 00:00:00

  • Extreme thermostability of tarantula hemocyanin.

    abstract::Biotops with extreme temperatures such as deserts force animals to avoid or escape high temperatures by biochemical, behavioural or morphological adaptation. In this context we tested the resistance to heat of the oxygen carrier hemocyanin from the ancient tarantula Eurypelma californicum, which is found in arid zones...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sterner R,Vogl T,Hinz HJ,Penz F,Hoff R,Föll R,Decker H

    更新日期:1995-05-01 00:00:00

  • A constitutive form of heat-shock protein 70 is located in the outer membranes of mitochondria from rat liver.

    abstract::HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lithgow T,Ryan M,Anderson RL,Høj PB,Hoogenraad NJ

    更新日期:1993-10-18 00:00:00

  • The role of mild uncoupling and non-coupled respiration in the regulation of hydrogen peroxide generation by plant mitochondria.

    abstract::The roles of mild uncoupling caused by free fatty acids (mediated by plant uncoupling mitochondrial protein (PUMP) and ATP/ADP carrier (AAC)) and non-coupled respiration (alternative oxidase (AO)) on H(2)O(2) formation by plant mitochondria were examined. Both laurate and oleate prevent H(2)O(2) formation dependent on...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Casolo V,Braidot E,Chiandussi E,Macrì F,Vianello A

    更新日期:2000-05-26 00:00:00

  • Laminin alpha1 chain mediated reduction of laminin alpha2 chain deficient muscular dystrophy involves integrin alpha7beta1 and dystroglycan.

    abstract::Transgenically introduced laminin (LN) alpha1 chain prevents muscular dystrophy in LNalpha2 chain deficient mice. We now report increased integrin alpha7Bbeta1D synthesis in dystrophic LNalpha2 chain deficient muscle. Yet, immunofluorescence demonstrated a reduced expression of integrin alpha7B subunit at the sarcolem...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gawlik KI,Mayer U,Blomberg K,Sonnenberg A,Ekblom P,Durbeej M

    更新日期:2006-03-20 00:00:00

  • Structural variability of the ubiquitin specific protease DUSP-UBL double domains.

    abstract::USP4, 11 and 15 are three closely related paralogues of the ubiquitin specific protease (USP) family of deubiquitinating enzymes. The DUSP domain and the UBL domain in these proteins are juxtaposed which may provide a functional unit conferring specificity. We determined the structures of the USP15 DUSP-UBL double dom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Elliott PR,Liu H,Pastok MW,Grossmann GJ,Rigden DJ,Clague MJ,Urbé S,Barsukov IL

    更新日期:2011-11-04 00:00:00

  • On how hydrolysis at the 3' end is prevented in the splicing of a sequentially folded group I intron.

    abstract::We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fernández A

    更新日期:1992-02-03 00:00:00

  • Voltage sensor mutations differentially target misfolded K+ channel subunits to proteasomal and non-proteasomal disposal pathways.

    abstract::In Shaker K(+) channels, formation of an electrostatic interaction between two charged residues, D316 and K374 in transmembrane segments S3 and S4, respectively, is a key step in voltage sensor biogenesis. Mutations D316K and K374E disrupt formation of the voltage sensor and lead to endoplasmic reticulum retention. We...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Myers MP,Khanna R,Lee EJ,Papazian DM

    更新日期:2004-06-18 00:00:00

  • Why do pathway methods work better than they should?

    abstract::Pathway analysis methods are frequently applied to cancer gene expression data to identify dysregulated pathways. These methods often infer pathway activity based on the expression of genes belonging to a given pathway, even though the proteins ultimately determine the activity of a given pathway. Furthermore, the ass...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Szalai B,Saez-Rodriguez J

    更新日期:2020-12-01 00:00:00

  • Fluorescence of native and carotenoid-depleted LH2 from Chromatium minutissimum, originating from simultaneous two-photon absorption in the spectral range of the presumed (optically 'dark') S(1) state of carotenoids.

    abstract::Native and carotenoid-depleted peripheral purple bacterial light-harvesting complex (LH2) were investigated by simultaneous two-photon excited (between 1300-1500 nm) fluorescence (TPF). TPF results from direct bacteriochlorophyll excitation in both samples. The spectral position of the 2A(g)(-) state of rhodopin [corr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Krikunova M,Kummrow A,Voigt B,Rini M,Lokstein H,Moskalenko A,Scheer H,Razjivin A,Leupold D

    更新日期:2002-09-25 00:00:00

  • Cloning of the protein D2 gene of Pseudomonas aeruginosa and its functional expression in the imipenem-resistant host.

    abstract::Protein D2 forms the water-filled pore across the outer membrane of Pseudomonas aeruginosa and allows the penetration of imipenem. We cloned the protein D2 gene by the antibody screening technique. When the imipenem-resistant mutant lacking protein D2 harbored the plasmid with the cloned D2 gene, the mutant overproduc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoneyama H,Nakae T

    更新日期:1991-06-03 00:00:00

  • Cloning and sequence analysis of a cDNA encoding rice glutaredoxin.

    abstract::A full-length cDNA clone (RASC8) encoding glutaredoxin (thioltransferase) was isolated from a cDNA library of an aleurone layer prepared from a developing seed of rice (Oryza sativa L.). RASC8, 568bp in length, contained an ATG codon and two possible polyadenylation signals, and encoded 112 amino acid residues. Cys-Pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minakuchi K,Yabushita T,Masumura T,Ichihara K,Tanaka K

    更新日期:1994-01-10 00:00:00

  • Measurement of GTP gamma S binding to specific G proteins in membranes using G-protein antibodies.

    abstract::We developed a novel method to quantitatively measure GTP gamma S binding to specific G proteins in crude membranes using G-protein antibodies. The basic strategy was that the materials were initially incubated with [35S]GTP gamma S at 37 degrees C. After 4 degrees C incubation in the wells of an ELISA plate precoated...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Okamoto T,Ikezu T,Murayama Y,Ogata E,Nishimoto I

    更新日期:1992-06-29 00:00:00

  • Determination of the catalytic pathway of C4-leaf pyruvate, orthophosphate dikinase from maize.

    abstract::The mechanism of the maize pyruvate, phosphate dikinase-catalyzed conversion of adenosine 5'-triphosphate, orthophosphate and pyruvate to adenosine 5'-monophosphate, inorganic pyrophosphate and phosphoenolpyruvate, respectively, was determined by using transient kinetic techniques. The data obtained demonstrate that c...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Carroll LJ,Dunaway-Mariano D,Smith CM,Chollet R

    更新日期:1990-11-12 00:00:00

  • Effect of lysophosphatidic acid on the preimplantation development of mouse embryos.

    abstract::The effect of lysophosphatidic acid (LPA) on the preimplantation development of mouse embryos was examined. The blastocyst rates from 2-cell and 4-cell stage embryos were significantly higher in the presence of LPA at the pronuclear stage. However, the blastocyst rate from 4-cell stage embryos was significantly lower ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kobayashi T,Yamano S,Murayama S,Ishikawa H,Tokumura A,Aono T

    更新日期:1994-08-29 00:00:00

  • Novel nuclear-encoded proteins interacting with a plastid sigma factor, Sig1, in Arabidopsis thaliana.

    abstract::Sigma factor binding proteins are involved in modifying the promoter preferences of the RNA polymerase in bacteria. We found the nuclear encoded protein (SibI) that is transported into chloroplasts and interacts specifically with the region 4 of Sig1 in Arabidopsis. SibI and its homologue, T3K9.5 are novel proteins, w...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Morikawa K,Shiina T,Murakami S,Toyoshima Y

    更新日期:2002-03-13 00:00:00

  • Na+ + Cl- -gradient-driven, high-affinity, uphill transport of taurine in human placental brush-border membrane vesicles.

    abstract::Uptake of taurine in human placental brush-border membrane vesicles was greatly stimulated in the presence of an inwardly-directed Na+ + Cl- -gradient and uphill transport of taurine could be demonstrated under these conditions. Na+ as well as Cl- were obligatory for this uptake and both ion gradients could energize t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Miyamoto Y,Balkovetz DF,Leibach FH,Mahesh VB,Ganapathy V

    更新日期:1988-04-11 00:00:00

  • A pre-existing protease is a common effector of thymocyte apoptosis mediated by diverse stimuli.

    abstract::Data from a number of model systems support a role for proteolysis in apoptotic cell death. Using immature rat thymocytes, we demonstrate that the inhibitors N-tosyl-L-lysyl chloromethylketone (TLCK) and N-tosyl-L-phenylalanyl chloromethylketone (TPCK) have very different effects on apoptosis. TLCK inhibits apoptosis ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fearnhead HO,Rivett AJ,Dinsdale D,Cohen GM

    更新日期:1995-01-09 00:00:00

  • Structural domains in RNAi.

    abstract::Structural and biochemical studies have begun to elucidate the pathway of RNA silencing that leads to the formation of the RISC complex. The outstanding feature of this pathway is the precise recognition and processing of double-stranded RNA. We present a review of recent structures that illustrate the molecular mecha...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Collins RE,Cheng X

    更新日期:2005-10-31 00:00:00

  • Ovine colostrum nanopeptide affects amyloid beta aggregation.

    abstract::A colostral proline-rich polypeptide complex (PRP) consisting of over 30 peptides shows beneficial effects in Alzheimer's disease (AD) patients when administered in the form of sublinqual tablets called Colostrinin. The aim of the present studies was to investigate whether nanopeptide fragment of PRP (NP) - one of the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Janusz M,Woszczyna M,Lisowski M,Kubis A,Macała J,Gotszalk T,Lisowski J

    更新日期:2009-01-05 00:00:00

  • Stimulation of synaptosomal free radical production by fatty acids: relation to esterification and to degree of unsaturation.

    abstract::The ability of three fatty acids and their respective ethyl esters, to promote generation of reactive oxygen species (ROS), was compared in a preparation of rat brain synaptosomes. Arachidonic but not palmitic or linoleic acids promoted ROS generation. Ethyl esterification of each fatty acid significantly enhanced ROS...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bondy SC,Marwah S

    更新日期:1995-11-13 00:00:00

  • Characterization of a novel aphid prenyltransferase displaying dual geranyl/farnesyl diphosphate synthase activity.

    abstract::We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vandermoten S,Charloteaux B,Santini S,Sen SE,Béliveau C,Vandenbol M,Francis F,Brasseur R,Cusson M,Haubruge E

    更新日期:2008-06-11 00:00:00

  • Identification of a troponin-I like protein in platelet preparations as histone H2B.

    abstract::A tropomyosin-binding protein (app. Mr 17000) was detected in equine platelet preparations by a gel overlay technique. Its isolation, amino acid and partial sequence analyses have shown it to be histone H2B. As with a similar protein from pig platelet preparations [der Terrossian et al. (1983) FEBS Lett. 152, 202-206]...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Stewart DI,Golosinska K,Smillie LB

    更新日期:1983-06-27 00:00:00

  • Hetero-oligomerization of adenosine A1 receptors with P2Y1 receptors in rat brains.

    abstract::Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshioka K,Hosoda R,Kuroda Y,Nakata H

    更新日期:2002-11-06 00:00:00

  • Catechin derivatives: specific inhibitor for caspases-3, 7 and 2, and the prevention of apoptosis at the cell and animal levels.

    abstract::Tea-catechin derivatives are shown to inhibit activities of caspases-3, 2 and 7 in vitro, and prevented experimental apoptosis at the cell and animal levels. Epigallo-catechin-gallate showed the strongest inhibition at 1 x 10(-7)M to these caspases, but cysteine cathepsins and caspase-8 were not inhibited. Caspase-3 i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Katunuma N,Ohashi A,Sano E,Ishimaru N,Hayashi Y,Murata E

    更新日期:2006-02-06 00:00:00

  • Ser727-dependent transcriptional activation by association of p300 with STAT3 upon IL-6 stimulation.

    abstract::Activation of the signal transducer and activator of transcription 3 (STAT3) in response to interleukin-6 (IL-6) type cytokines involves both phosphorylation of Tyr705, which enables dimerization, nuclear translocation and DNA binding, as well as ser727 phosphorylation. Here, we describe that the 65 C-terminal amino a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schuringa JJ,Schepers H,Vellenga E,Kruijer W

    更新日期:2001-04-20 00:00:00

  • Resistance to tumor necrosis factor (TNF) cytotoxicity by autocrine TNF production is independent of intracellular signaling pathways.

    abstract::We previously showed that autocrine tumor necrosis factor (TNF) production in the TNF-sensitive L929sA fibrosarcoma cell line induced TNF resistance, which is correlated with downmodulation of both TNF receptors on the cell surface. We now analyzed whether autocrine TNF production also interfered with intracellular TN...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Decoster E,Vandevoorde V,Beyaert R,Haegeman G,Fiers W

    更新日期:1997-10-20 00:00:00

  • The C-terminal 26-residue peptide of serpin A1 stimulates proliferation of breast and liver cancer cells: role of protein kinase C and CD47.

    abstract::C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Congote LF,Temmel N

    更新日期:2004-10-22 00:00:00