Abstract:
:The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they do not affect plant protein synthesis under the same conditions. Therefore, they should also be considered as true RIPs but of a new class, the four-chain RIPs. An extended classification of RIPs is presented based on the former one from Stirpe et al. [Bio/technology 10 (1992) 405-412].
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Citores L,Ferreras JM,Iglesias R,Carbajales ML,Arias FJ,Jiménez P,Rojo MA,Girbés Tdoi
10.1016/0014-5793(93)80193-xsubject
Has Abstractpub_date
1993-08-23 00:00:00pages
59-62issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80193-Xjournal_volume
329pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.12.029
更新日期:2013-04-17 00:00:00
abstract::Recombinant cell lines expressing D2 and D3 dopamine receptor isoforms have been used to study coupling of these receptors to G-proteins from the effects of GTP to reduce agonist binding affinities in agonist/[3H]spiperone competition experiments. D2(short) expressed in Ltk- cells, D2(long) expressed in Ltk- or CHO ce...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81168-y
更新日期:1993-01-11 00:00:00
abstract::The Ca2+-binding parvalbumin has been purified for the first time from rat kidney. Its biochemical and immunological properties were indistinguishable from the muscle counterpart. By immunohistochemical methods parvalbumin was localized in part of the distal tubule and proximal collecting duct, similar to the vitamin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80569-5
更新日期:1986-05-26 00:00:00
abstract::Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80070-j
更新日期:1991-01-14 00:00:00
abstract::We have investigated the availability of zinc in the periplasmic space of Escherichia coli using a mutant Cu,Zn superoxide dismutase whose dimerization is triggered by zinc binding. This mutant enzyme accumulates in the monomeric form when wild type cells are grown in minimal medium, but assembles in the dimeric form ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.008
更新日期:2004-07-02 00:00:00
abstract::The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81341-i
更新日期:1992-10-05 00:00:00
abstract::In tritium-hydrogen exchange experiments, the large GroEL substrate Rubisco was unfolded and exchanged in urea/acid/tritiated water, then diluted into either protic buffer or protic buffer containing GroEL. The respective Rubisco metastable folding intermediate or Rubisco-GroEL binary complex was then separated from r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.013
更新日期:2005-02-14 00:00:00
abstract::The effects of guanine nucleotides on arachidonic acid (AA) release were studied in intact and saponin-permeabilized human platelets. While GTP[S] itself caused a stimulation of AA release in permeabilized cells, GTP[S], GDP[S], GTP, ATP and other nucleotides inhibited AA release in response to thrombin and other agon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80107-8
更新日期:1988-07-18 00:00:00
abstract::Male germ cell Rac GTPase-activating protein (MgcRacGAP) is a core regulator of cytokinesis. Furthermore, it appears to be involved in human oncogenesis through cytokinesis-independent mechanisms and has been reported to be essential for nuclear translocation of signal transducer and activator of transcription (STAT) ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.11.013
更新日期:2015-12-21 00:00:00
abstract::The role of protein kinase C (PKC) in cellular regulation of L-type Ca2+ channels was investigated in human umbilical vein smooth muscle. Activation of PKC, by low concentrations (< 30 nM) of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) caused inhibition of Ca2+ channels, while higher concentrations of TPA (> 100 nM) e...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80458-3
更新日期:1994-03-21 00:00:00
abstract::The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochond...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80622-5
更新日期:1984-12-10 00:00:00
abstract::Muscle phosphofructokinase interacts with microtubule-associated protein-free microtubules resulting in a reduction of the overall activity of the enzyme [Lehotzky et al. (1993) J. Biol. Chem. 268, 10888-10894] and periodical cross-linking of the tubules [Lehotzky et al. (1994) Biochem. Biophys. Res. Commun. 204, 585-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01510-8
更新日期:1996-01-29 00:00:00
abstract::The phylogenetic distribution of divergently related protein families into the three domains of life (archaea, bacteria and eukaryotes) can signify the presence or absence of entire cellular processes in these domains and their ancestors. We can thus study the emergence of the major transitions during cellular evoluti...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(96)00631-x
更新日期:1996-07-22 00:00:00
abstract::Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00012-x
更新日期:1998-01-30 00:00:00
abstract::The beta-adrenergic receptor kinase mediates agonist-dependent phosphorylation of beta-adrenergic receptors, which is thought to represent the first step of homologous desensitization. We have expressed bovine and human beta ARK1 in Sf9 cells and purified them to apparent homogeneity in milligram quantities. The Km-va...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81532-5
更新日期:1993-06-07 00:00:00
abstract::Respiratory supercomplex factor (Rcf) 1 is a membrane-bound protein that modulates the activity of cytochrome c oxidase (CytcO) in Saccharomyces cerevisiae mitochondria. To investigate this regulatory mechanism, we studied the interactions of CytcO with potassium cyanide (KCN) upon removal of Rcf1. While the addition ...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12918
更新日期:2017-12-01 00:00:00
abstract::Epidermal growth factor (EGF) dose-dependently enhanced the induction of tyrosine aminotransferase and tryptophan oxygenase by glucocorticoids in primary cultures of adult rat hepatocytes without itself having any effect on these enzymes in the absence of glucocorticoids. The amplifications were observed even with dex...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80293-4
更新日期:1987-11-02 00:00:00
abstract::DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00136-2
更新日期:1999-02-26 00:00:00
abstract::Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00084-5
更新日期:2004-02-27 00:00:00
abstract::Several myeloid precursors of antibacterial peptides have recently been shown to share homologous pre- and pro-regions. Taking advantage of this homology, a novel cDNA was cloned from pig bone marrow RNA. This encodes a 166-residue polypeptide with highly conserved pre- (29 residues) and pro- (101 residues) sequences,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80214-9
更新日期:1994-01-17 00:00:00
abstract::The less polar fluorescent analogue of ATP, 2'(3')-trinitrophenyl-5'-triphosphate bound to rat liver fatty acid-binding protein with high affinity (Kd 6.3 x 10(-6) M) and 1:1 molar stoichiometry. This probe bound to the fatty acid binding site of the protein and was displaced by oleic acid and oleoyl CoA. High concent...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81532-q
更新日期:1992-12-21 00:00:00
abstract::Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00348-d
更新日期:1995-05-01 00:00:00
abstract::The role of two positively charged amino acid residues located at the active site of Escherichia coli maltodextrin phosphorylase was investigated by site-directed mutagenesis. Substitution of Lys539 by an arginine caused a 600-fold reduction, substitution of Arg534 by a glutamine caused an even larger 7000-fold reduct...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80956-4
更新日期:1991-07-29 00:00:00
abstract::Stopped-flow kinetics showed that the inhibition of the lysosomal cysteine proteinase, cathepsin B, by its endogenous inhibitor, cystatin C, occurs by a two-step mechanism, in which an initial, weak interaction is followed by a conformational change. The initial interaction most likely involves binding of the N-termin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01604-9
更新日期:1998-01-23 00:00:00
abstract::Oligopeptides derived from the gag polyprotein (Pr55gag) of human immunodeficiency virus type 1 (HIV-1) segment were used to evaluate the extension of the putative binding region for the complex of Pr55gag and the human cytosolic peptidyl prolyl cis/trans isomerase (PPIase) 18 kDa cyclophilin (Cyp18). Five N-terminall...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00972-6
更新日期:1996-10-07 00:00:00
abstract::The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80398-3
更新日期:1988-05-09 00:00:00
abstract::Properties of Klebsiella pneumoniae respiratory chain enzymes catalyzing NADH oxidation have been studied. Using constructed K. pneumoniae mutant strains, it was shown that three enzymes belonging to different families of NADH:quinone oxidoreductases operate in this bacterium. The NDH-2-type enzyme is not coupled with...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00312-6
更新日期:2004-04-09 00:00:00
abstract::Acylphosphatase (AcP) activity in prokaryotes was classically attributed to some aspecific acid phosphatases. We identified an open reading frame for a putative AcP in the b0968 Escherichia coli gene and purified the recombinant enzyme after checking by RT-PCR that it was indeed expressed. EcoAcP has a predicted typic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.033
更新日期:2006-12-22 00:00:00
abstract::Secreted proteins are indispensable for the development and differentiation of multicellular organisms. Cloning and characterization of novel or hypothetical genes encoding these proteins are therefore inviting great incentives. Using bioinformatics tools and experimental approaches, we isolated and characterized a hu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.059
更新日期:2004-07-30 00:00:00
abstract::Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81075-9
更新日期:1985-01-28 00:00:00