Abstract:
:The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they do not affect plant protein synthesis under the same conditions. Therefore, they should also be considered as true RIPs but of a new class, the four-chain RIPs. An extended classification of RIPs is presented based on the former one from Stirpe et al. [Bio/technology 10 (1992) 405-412].
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Citores L,Ferreras JM,Iglesias R,Carbajales ML,Arias FJ,Jiménez P,Rojo MA,Girbés Tdoi
10.1016/0014-5793(93)80193-xsubject
Has Abstractpub_date
1993-08-23 00:00:00pages
59-62issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(93)80193-Xjournal_volume
329pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Amylin appears to interfere with the action of insulin in muscle and possibly in liver. We have attempted to detect a direct antagonism between amylin and insulin in cultured rat hepatocytes. The stimulation of glucokinase gene expression was used as a marker of insulin action. Amylin proved ineffective in suppressing...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80222-3
更新日期:1992-04-13 00:00:00
abstract::The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The iden...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00934-1
更新日期:1994-09-26 00:00:00
abstract::The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80968-7
更新日期:1987-09-14 00:00:00
abstract::A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80835-q
更新日期:1991-11-04 00:00:00
abstract::We report phosphorylated and ubiquitinated aggregates of TAR DNA binding protein of 43 kDa (TDP-43) in SH-SY5Y cells similar to those in brains of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U). Two candidate sequences for the nuclear localization signa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.12.031
更新日期:2009-01-22 00:00:00
abstract::Small RNAs are non-coding regulatory RNA molecules that control gene expression by mediating mRNA degradation, translational inhibition, or chromatin modification. Virus-derived small RNAs induce silencing of viral RNAs and are essential for antiviral defense in both animal and plant systems. The role of host endogeno...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2008.06.053
更新日期:2008-08-06 00:00:00
abstract::The incorporation of carbon into the carbon monoxide and cyanide ligands of [NiFe]-hydrogenases has been investigated by using (13)C labelling in infrared studies of the Allochromatium vinosum enzyme and by (14)C labelling experiments with overproduced Hyp proteins from Escherichia coli. The results suggest that the b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.12.013
更新日期:2005-01-17 00:00:00
abstract::32 consensus patterns for a set of functional regions and structural motifs in protein sequences were constructed. The pattern definition is heuristic and based on 11 selected steric and physicochemical properties. By comparison with these patterns, it was possible to identify, without false detection, 1532 sites in 8...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81818-6
更新日期:1989-10-23 00:00:00
abstract::Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/auto...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.09.009
更新日期:2010-10-08 00:00:00
abstract::In order to investigate oxysterol-mediated effects on the biliary system, we studied the effects of cholestan-3beta,5alpha,6beta-triol (TriolC) and 7-ketocholesterol (7KC) on gallbladder epithelial cells. We compared their cell proliferation effects in cultured dog gallbladder epithelial cells (DGBE) to their effects ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01831-7
更新日期:2000-07-28 00:00:00
abstract::A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80523-w
更新日期:1993-03-08 00:00:00
abstract::Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00576-1
更新日期:1999-06-18 00:00:00
abstract::Previously, we found that intracellular reactive oxygen species (ROS) affect photomorphogenesis in Neurospora crassa. In this study, we investigated the physiological roles of ROS in the response to light and found that the exposure of mycelia to air was important for the light-induced carotenogenesis. Mycelia treated...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.06.014
更新日期:2005-07-18 00:00:00
abstract::Here we report that splice blocking morpholinos (Sb MO) against zebrafish sox31 elicit developmental arrest, likely through creating a series of dominant negative splicing variants. Embryos injected with the Sb MO develop normally before the Mid-Blastula Transition (MBT); however, they do not initiate epiboly. Microar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.12.008
更新日期:2012-02-03 00:00:00
abstract::Treatment of rat liver microsomes with 2,5-di(tert-butyl)-1,4-benzohydroquinone caused a dose-related inhibition (Ki congruent to 1 microM) of ATP-dependent Ca2+ sequestration. This was paralleled by a similar impairment of the microsomal Ca2+-stimulated ATPase activity. In contrast, the hydroquinose failed to induce ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80479-9
更新日期:1987-11-30 00:00:00
abstract::Iron dependent regulator, IdeR, regulates the expression of genes in response to intracellular iron levels in M. tuberculosis. Orthologs of IdeR are present in all the sequenced genomes of mycobacteria. We have used a computational approach to identify conserved IdeR regulated genes across the mycobacteria and the gen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.090
更新日期:2006-05-15 00:00:00
abstract::The initial processes of platelet activation by platelet-activating factor (PAF) were observed by stopped-flow light scattering and fluorometry. The binding of PAF to rabbit platelets did not change the membrane fluidity, though it caused the removal of calcium from internal stores and induced concomitantly platelet s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80034-5
更新日期:1986-10-20 00:00:00
abstract::Hepatocyte tight junctional permeability has been shown to be regulated by hormones that exert their effects via phospholipase C activation. However, the precise transduction pathway involved in this effect is not known. The present study has employed the selective inhibitor of microsomal Ca2+ sequestration, 2,5-di(te...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80209-l
更新日期:1991-03-11 00:00:00
abstract::The amino acid residue Asn141 of the restriction endonuclease EcoRI was proposed to make three hydrogen bonds to both adenine residues within the recognition sequence -GAATTC-. We have mutated Asn141 to alanine, aspartate, serine, and tyrosine. Only the serine mutant is active under normal buffer conditions although 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01274-5
更新日期:1998-10-30 00:00:00
abstract::A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00265-7
更新日期:1996-04-01 00:00:00
abstract::A new phagemid cloning vector for positive selection of recombinants, pBa-7, was constructed which contains an active barnase gene encoding the cytotoxic ribonuclease from Bacillus amyloliquefaciens, under control of the lac promoter. PBa-7 is a derivative of the high-copy number pBluescript II KS+ phagemid in which t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00661-4
更新日期:1999-06-11 00:00:00
abstract::Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03722-5
更新日期:2002-12-18 00:00:00
abstract::The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80502-7
更新日期:1988-10-10 00:00:00
abstract::Criteria for the design of peptide vaccines to prevent AIDS are presented. The best vaccine candidates contain both B and T lymphocyte-defined epitopes in regions conserved in sequence between viral isolates. We propose that attention should focus on proteins specified by the gag and, possibly, pol genes in addition t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81052-9
更新日期:1987-06-29 00:00:00
abstract::We report on the cDNA cloning and characterization of a novel short-chain isoprenyl diphosphate synthase from the aphid Myzus persicae. Of the three IPPS cDNAs we cloned, two yielded prenyltransferase activity following expression in Escherichia coli; these cDNAs encode identical proteins except for the presence, in o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.043
更新日期:2008-06-11 00:00:00
abstract::c-Myc is a transcriptional regulator involved in carcinogenesis through its role in growth control and cell cycle progression. Here we attempt to relate its role in stimulating the G1-S transition to the ability to affect functioning of key cell cycle regulators, and we focus on how its property of modulating transcri...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02118-4
更新日期:2001-02-16 00:00:00
abstract::SufC is an ATPase component of the SUF machinery, which is involved in the biosynthesis of Fe-S clusters. To gain insight into the function of this protein, we have determined the crystal structure of Escherichia coli SufC at 2.5A resolution. Despite the similarity of the overall structure with ABC-ATPases (nucleotide...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.058
更新日期:2006-01-09 00:00:00
abstract::Mechanisms involved in cell volume regulation are important in SS, SC cells as they might be involved in determining the extent of sickling and the generation of dense cells and irreversibly sickled cells. We have studied in these cells the response to cell swelling of the K+,Cl- transporter. We found that Hb SS, SC a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80538-5
更新日期:1986-05-05 00:00:00
abstract::The oxysterols, 7beta-hydroxycholesterol and 7-ketocholesterol, are involved in the cytotoxicity of oxidized LDL. To elucidate their molecular mechanisms, the human promonocytic leukemia cells U937 and U4 were used. U4 cells overexpressing Bcl-2 were obtained by transfection of U937 cells. 7Beta-hydroxycholesterol and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01473-7
更新日期:1997-12-15 00:00:00
abstract::When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13481
更新日期:2019-07-01 00:00:00