Periplasmic competition for zinc uptake between the metallochaperone ZnuA and Cu,Zn superoxide dismutase.

abstract：:Free radicals, including dopamine (DA)-oxidized metabolites, have long been implicated in pathogenesis of Parkinson's disease (PD). However, the relationships between such oxidative stresses and alpha-synuclein (alpha-S), a major constituent of Lewy bodies, remain unknown. In this study, we established neuronal cells ...

journal_title：FEBS letters

authors： Hasegawa T,Matsuzaki-Kobayashi M,Takeda A,Sugeno N,Kikuchi A,Furukawa K,Perry G,Smith MA,Itoyama Y

更新日期：2006-04-03 00:00:00

abstract：:The mitochondrial alternative oxidase is a diiron carboxylate quinol oxidase (Dox) found in plants and some fungi and protists, but not animals. The plastid terminal oxidase is distantly related to alternative oxidase and is most likely also a Dox protein. Database searches revealed that the alpha-proteobacterium Novo...

journal_title：FEBS letters

authors： Finnegan PM,Umbach AL,Wilce JA

更新日期：2003-12-18 00:00:00

abstract：:Nucleoside modifications in tRNA anticodons regulate ribosome dynamics during translation elongation and, thereby, fine-tune global protein synthesis rates. The highly conserved eukaryotic Elongator complex conducts specific C5-substitutions in tRNA wobble base uridines. It harbors two copies of each of its six indivi...

journal_title：FEBS letters

authors： Dauden MI,Jaciuk M,Müller CW,Glatt S

更新日期：2018-02-01 00:00:00

abstract：:Stability of green fluorescent protein (GFP) is sometimes important for a proper practical application of this protein. Random mutagenesis and targeted mutagenesis have been used to create better-folded variants of GFP, including recently reported extra-superfolder GFP. Our aim was to determine the crystal structure o...

journal_title：FEBS letters

authors： Choi JY,Jang TH,Park HH

更新日期：2017-01-01 00:00:00

abstract：:Fatty acid (FA) uniport via mitochondrial uncoupling protein (UcP) was detected fluorometrically with PBFI, potassium-binding benzofuran phthalate and SPQ, 6-methoxy-N-(3-sulfopropyl)-quinolinium, indicating K+ and H+, respectively. The FA structural patterns required for FA flip-flop, UcP-mediated FA uniport, activat...

journal_title：FEBS letters

authors： Jezek P,Modrianský M,Garlid KD

更新日期：1997-05-19 00:00:00

abstract：:Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...

journal_title：FEBS letters

authors： Feuerbach D,Feyen JH

更新日期：1997-01-27 00:00:00

abstract：:Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...

journal_title：FEBS letters

authors： Chen H,Wu Y,Zhang Y,Jin L,Luo L,Xue B,Lu C,Zhang X,Yin Z

更新日期：2006-05-29 00:00:00

abstract：:The A-rich bulge in paired region P5a of the Tetrahymena intron is a structural feature that is conserved in the sub-group Ib self-splicing introns. We have constructed a series of substitution and deletion mutations in this region of the intron. Kinetic analysis has shown that some of the mutants have a reduced maxim...

journal_title：FEBS letters

authors： Pace U,Szostak JW

更新日期：1991-03-11 00:00:00

abstract：:Recent studies indicate that junctophilin (JP) subtypes contribute to the formation of the junctional membrane complexes between the plasma membrane and the endoplasmic/sarcoplasmic reticulum (ER/SR) in excitable cells. Cardiac muscle contains the diad, in which the transverse (T) tubule of the invaginated cell membra...

journal_title：FEBS letters

authors： Komazaki S,Nishi M,Takeshima H

更新日期：2003-05-08 00:00:00

abstract：:We report that the mitotic inhibitor, vinblastine (VLB), is highly toxic to the malarial parasite, Plasmodium falciparum. In cultures in vitro growth is inhibited by 50% at a VLB level of about 28 nM, and totally abolished at a level of 100 nM. By tests on synchronized cultures we have found that the effect of VLB tak...

journal_title：FEBS letters

authors： Usanga EA,O'Brien E,Luzzato L

更新日期：1986-12-01 00:00:00

abstract：:Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...

journal_title：FEBS letters

authors： Charnet P,Bourinet E,Dubel SJ,Snutch TP,Nargeot J

更新日期：1994-05-09 00:00:00

abstract：:Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...

journal_title：FEBS letters

authors： Guimond A,Sulea T,Zwaagstra JC,Ekiel I,O'Connor-McCourt MD

更新日期：2002-02-27 00:00:00

abstract：:Reg, first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet beta-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, the Reg family. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and H...

journal_title：FEBS letters

authors： Miyashita H,Nakagawara K,Mori M,Narushima Y,Noguchi N,Moriizumi S,Takasawa S,Yonekura H,Takeuchi T,Okamoto H

更新日期：1995-12-27 00:00:00

abstract：:Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...

journal_title：FEBS letters

authors： Petersen TE

更新日期：1988-04-11 00:00:00

abstract：:Flavonoid 3'-hydroxylase (F3'H) and flavonoid 3',5'-hydroxylase (F3'5'H) are cytochrome P450 enzymes and determine the B-ring hydroxylation pattern of flavonoids by introducing hydroxyl groups at the 3'- or the 3'- and 5'-position, respectively. Sequence identity between F3'H and F3'5'H is generally low since their di...

journal_title：FEBS letters

authors： Seitz C,Ameres S,Forkmann G

更新日期：2007-07-24 00:00:00

abstract：:The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...

journal_title：FEBS letters

authors： Laliberté F,Liu S,Gorseth E,Bobechko B,Bartlett A,Lario P,Gresser MJ,Huang Z

更新日期：2002-02-13 00:00:00

abstract：:We have isolated cDNA molecules encoding a protein with the characteristic sequence elements that are conserved between the catalytic domains of protein kinases. This protein is apparently a serine/threonine kinase and is most closely related to the amino-terminal half of the ribosomal protein S6 kinase II first chara...

journal_title：FEBS letters

authors： Harmann B,Kilimann MW

更新日期：1990-10-29 00:00:00

abstract：:Spectrin repeats are three-helix bundle structures which occur in a large number of diverse proteins, either as single copies or in tandem arrangements of multiple repeats. They can serve structural purposes, by coordination of cytoskeletal interactions with high spatial precision, as well as a 'switchboard' for inter...

journal_title：FEBS letters

authors： Djinovic-Carugo K,Gautel M,Ylänne J,Young P

更新日期：2002-02-20 00:00:00

abstract：:The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 greater than CCK33 greater than desulphated CCK8 greater than CCK4. Binding of CCK to its receptor was abolish...

journal_title：FEBS letters

authors： Sakamoto C,Williams JA,Wong KY,Goldfine ID

更新日期：1983-01-10 00:00:00

abstract：:Myosin light chain kinase (MLCK) is the key regulator of cell motility and smooth muscle contraction in higher vertebrates. We searched for the features of the high molecular weight MLCK (MLCK-210) associated with its unique N-terminal sequence not found in a more ubiquitous lower molecular weight MLCK (MLCK-108). MLC...

journal_title：FEBS letters

authors： Kudryashov DS,Chibalina MV,Birukov KG,Lukas TJ,Sellers JR,Van Eldik LJ,Watterson DM,Shirinsky VP

更新日期：1999-12-10 00:00:00

abstract：:The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...

journal_title：FEBS letters

authors： Galietta LJ,Haggie PM,Verkman AS

更新日期：2001-06-22 00:00:00

abstract：:The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...

journal_title：FEBS letters

authors： Kunugi S,Yokoyama M,Sakamoto A

更新日期：1995-04-03 00:00:00

abstract：:The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA ...

journal_title：FEBS letters

authors： Holck A,Kleppe K

更新日期：1985-06-03 00:00:00

abstract：:Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

journal_title：FEBS letters

authors： Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

更新日期：2001-11-23 00:00:00

abstract：:Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...

journal_title：FEBS letters

authors： Kim SM,Kim JY,Lee S,Park JH

更新日期：2010-01-04 00:00:00

abstract：:Proline residues are rarely found in the three most C-terminal positions of bacterial signal peptides, and have never been found in position +1 immediately following the cleavage site. It was recently shown that a Pro+1 mutation in the E. coli maltose binding protein precursor not only prevents cleavage of the signal ...

journal_title：FEBS letters

authors： Nilsson I,von Heijne G

更新日期：1992-03-16 00:00:00

abstract：:31P-NMR studies of Mycoplasma gallisepticum cells have been carried out using a continuous perfusion technique; these are the first such studies with this organism. Using this technique, glucose metabolism was monitored in the intact organisms, and cell extracts were prepared to identify the intermediates. Under glyco...

journal_title：FEBS letters

authors： Egan W,Barile M,Rottem S

更新日期：1986-08-18 00:00:00

abstract：:The RhoGTPase Rac1 is activated in a polarised fashion and controls cell motility. We previously showed that Rac1 binds the PP2A inhibitor SET and recruits nuclear SET to the cytosol. We show that a SET mutant, lacking a nuclear localization signal, SET(ΔNLS), promotes cell spreading and motility. This was accompanied...

journal_title：FEBS letters

authors： Lam BD,Anthony EC,Hordijk PL

更新日期：2013-01-16 00:00:00

abstract：:Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known ...

journal_title：FEBS letters

authors： Schlenzka W,Shaw L,Kelm S,Schmidt CL,Bill E,Trautwein AX,Lottspeich F,Schauer R

更新日期：1996-05-06 00:00:00

abstract：:In rat cerebrocortical synaptosomes, the addition of 4 beta-phorbol dibutyrate (4 beta-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or pre-incubation with staurosporine, pre...

journal_title：FEBS letters

authors： Herrero I,Miras-Portugal MT,Sánchez-Prieto J

更新日期：1992-01-27 00:00:00