Two-step mechanism of inhibition of cathepsin B by cystatin C due to displacement of the proteinase occluding loop.

abstract：:Immunoprecipitated p100Gag-Mil protein kinase from MH2-transformed quail embryo fibroblasts is associated with an 89 kDa protein. The molar ratio between p89 and Gag-Mil in the immunocomplex is 0.72, indicating that the majority of Gag-Mil is complexed with p89. During mitosis part of Gag-Mil is shifted to a form with...

journal_title：FEBS letters

authors： Lovrić J,Bischof O,Moelling K

更新日期：1994-04-18 00:00:00

abstract：:Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be requi...

journal_title：FEBS letters

authors： Esyunina D,Pupov D,Kulbachinskiy A

更新日期：2019-02-01 00:00:00

abstract：:Mice are widely used for exploring obesity physiology and treatment. However, thermal biology is different between small and large mammals. In this Review, we discuss how the effect of environmental temperature must be understood to ensure applicability of mouse experiments to human obesity. At ambient environmental t...

journal_title：FEBS letters

authors： Reitman ML

更新日期：2018-06-01 00:00:00

abstract：:Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

journal_title：FEBS letters

authors： Manjithaya R,Nazarko TY,Farré JC,Subramani S

更新日期：2010-04-02 00:00:00

abstract：:The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using 1H-1H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the alpha(1-3) antenna is shown to exist in a preferred solution conformation wit...

journal_title：FEBS letters

authors： Homans SW,Dwek RA,Fernandes DL,Rademacher TW

更新日期：1982-12-27 00:00:00

abstract：:Friedreich ataxia is the most common recessive neurodegenerative disease and is caused by reduced expression of mitochondrial frataxin. Frataxin depletion causes impairment in iron-sulfur cluster and heme biosynthesis, disruption of iron homeostasis and hypersensitivity to oxidants. Currently no pharmacological treatm...

journal_title：FEBS letters

authors： Lefevre S,Sliwa D,Auchère F,Brossas C,Ruckenstuhl C,Boggetto N,Lesuisse E,Madeo F,Camadro JM,Santos R

更新日期：2012-01-20 00:00:00

abstract：:A novel gene named fad104 (factor for adipocyte differentiation-104), whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. The expr...

journal_title：FEBS letters

authors： Tominaga K,Kondo C,Johmura Y,Nishizuka M,Imagawa M

更新日期：2004-11-05 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:Cells of Saccharomyces cerevisiae were loaded with indo-1, by incubation in a medium of pH 4.5, which contained penta-potassium indo-1. Cells were then washed and resuspended in a buffer of pH 4.0. The emission fluorescence spectra were recorded between 390 and 500 nm (excitation at 355 nm) and the autofluorescent spe...

journal_title：FEBS letters

authors： Halachmi D,Eilam Y

更新日期：1989-10-09 00:00:00

abstract：:The sequence of the 18 S rRNA of Tenebrio molitor is reported. A detailed secondary structure model for eukaryotic small subunit rRNAs is proposed. The model comprises 48 universal helices that eukaryotic and prokaryotic small subunit rRNAs have in common, plus a number of helices in areas of variable secondary struct...

journal_title：FEBS letters

authors： Hendriks L,De Baere R,Van Broeckhoven C,De Wachter R

更新日期：1988-05-09 00:00:00

abstract：:Under destabilising conditions both heat and cold denaturation of yeast phosphoglycerate kinase (PGK) can be observed. According to previous interpretation of experimental data and theoretical calculations, the C-terminal domain should be more stable than the N-terminal domain at all temperatures. We report on thermal...

journal_title：FEBS letters

authors： Gast K,Damaschun G,Desmadril M,Minard P,Müller-Frohne M,Pfeil W,Zirwer D

更新日期：1995-01-30 00:00:00

abstract：:A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

journal_title：FEBS letters

authors： Lopina OD,Pindel EV,Boldyrev AA

更新日期：1990-06-18 00:00:00

abstract：:We have found that SV40 infection of CV1 cells induces the synthesis of a 72 kDa protein that upon molecular cloning was shown to be the product of the hsc70 gene. The above gene product was found to be mainly virus inducible, in contrast to the hsp70 gene product which was mainly heat inducible. The two genes were fo...

journal_title：FEBS letters

authors： Sainis I,Angelidis C,Pagoulatos G,Lazaridis I

更新日期：1994-12-05 00:00:00

abstract：:To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...

journal_title：FEBS letters

authors： Hagiwara Y,Nureki O,Tateno M

更新日期：2009-02-18 00:00:00

abstract：:Pasteurella multocida toxin is a potent mitogen for cultured Swiss 3T3 cells where it causes an accumulation of inositol phosphates and activation of protein kinase C. The gene sequence described here coded for a 146 kDa protein. The ORF was preceded by a ribosome binding site and followed by a stem loop. There was no...

journal_title：FEBS letters

authors： Lax AJ,Chanter N,Pullinger GD,Higgins T,Staddon JM,Rozengurt E

更新日期：1990-12-17 00:00:00

abstract：:The contact-dependent intrinsic pathway of fibrinolysis involving factor XII, prekallikrein (PK) and pro-urokinase (pro-UK) remains poorly understood. Casein autography of washed, intact platelets revealed both PK and pro-UK. Accordingly, platelets may mediate physiological thrombolysis by this pathway since factor XI...

journal_title：FEBS letters

authors： Gurewich V,Johnstone M,Loza JP,Pannell R

更新日期：1993-03-08 00:00:00

abstract：:The role of retinoic acids (RA) on liver fatty acid-binding protein (L-FABP) expression was investigated in the well differentiated FAO rat hepatoma cell line. 9-cis-Retinoic acid (9-cis-RA) specifically enhanced L-FABP mRNA levels in a time- and dose-dependent manner. The higher induction was found 6 h after addition...

journal_title：FEBS letters

authors： Poirier H,Braissant O,Niot I,Wahli W,Besnard P

更新日期：1997-08-04 00:00:00

abstract：:Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

journal_title：FEBS letters

authors： Yu HJ,Unnerstall JR,Green RD

更新日期：1995-10-23 00:00:00

abstract：:The verprolin family of proteins, WIP, CR16 and WIRE/WICH, has emerged as critical regulators of cytoskeletal organisation in vertebrate cells. The founding father of the family, verprolin, was originally identified in budding yeast and later shown to be needed for actin polymerisation during polarised growth and duri...

journal_title：FEBS letters

authors： Aspenström P

更新日期：2005-10-10 00:00:00

abstract：:We report that the mitotic inhibitor, vinblastine (VLB), is highly toxic to the malarial parasite, Plasmodium falciparum. In cultures in vitro growth is inhibited by 50% at a VLB level of about 28 nM, and totally abolished at a level of 100 nM. By tests on synchronized cultures we have found that the effect of VLB tak...

journal_title：FEBS letters

authors： Usanga EA,O'Brien E,Luzzato L

更新日期：1986-12-01 00:00:00

abstract：:The function of the coenzyme Q (CoQ) pool in the inner mitochondrial membrane is reviewed in view of recent findings suggesting a supramolecular organization of the mitochondrial respiratory complexes. In spite of the structural evidence for preferential aggregations of the inner membrane components, most kinetic evid...

journal_title：FEBS letters

authors： Lenaz G

更新日期：2001-12-07 00:00:00

abstract：:The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced m...

journal_title：FEBS letters

authors： Zhou JH,Baba T,Takano T,Kobayashi S,Arai Y

更新日期：1989-09-11 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:The kinetics of AMP-aminohydrolase, which under steady state conditions shows a typical sigmoid dependence of initial velocities versus substrate concentration, have been examined by rapid mixing methods. Using this technique it was observed that when substrate or substrate plus activator (K(+)) were mixed with enzyme...

journal_title：FEBS letters

authors： Suelter CH,Kovacs AL,Antonini E

更新日期：1968-11-01 00:00:00

abstract：:Phospholipase C (PLC) was purified from squid retina. Soluble Gq alpha, membrane Gq alpha and G beta gamma were isolated from GTP gamma S-treated and light-illuminated photoreceptor membranes. The membrane Gq alpha stimulated phosphatidyl inositol-phospholipase C (PI-PLC) activity in a dose-dependent manner. Soluble G...

journal_title：FEBS letters

authors： Suzuki T,Terakita A,Narita K,Nagai K,Tsukahara Y,Kito Y

更新日期：1995-12-27 00:00:00

abstract：:The Pb, Eu and Mg-induced cleavages in E. coli and lupine tRNAPhe have been characterized and compared with those found in yeast tRNAPhe. The pattern of lupine tRNAPhe hydrolysis closely resembles that of yeast tRNAPhe, while several major differences occur in the specificity and efficiency of the E. coli tRNAPhe hydr...

journal_title：FEBS letters

authors： Marciniec T,Ciesiołka J,Wrzesinski J,Krzyzosiak WJ

更新日期：1989-01-30 00:00:00

abstract：:By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumpi...

journal_title：FEBS letters

authors： Kashuba VI,Gizatullin RZ,Protopopov AI,Allikmets R,Korolev S,Li J,Boldog F,Tory K,Zabarovska V,Marcsek Z,Sumegi J,Klein G,Zabarovsky ER,Kisselev L

更新日期：1997-12-15 00:00:00

abstract：:The in vitro methylation of the +6, +24, and -215 located 5'-CCGG-3' sequences in the late E2A promoter of adenovirus type 2 (Ad2) DNA abrogates promoter function. 5-Methyldeoxycytidine (5-mC) at positions +6 and +24 in both or either of the two DNA complements in the late E2A promoter abolishes the formation of a hig...

journal_title：FEBS letters

authors： Hermann R,Doerfler W

更新日期：1991-04-09 00:00:00

abstract：:Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

journal_title：FEBS letters

authors： Shuvaev VV,Siest G

更新日期：1996-03-25 00:00:00

abstract：:A human teratoma cell line (Tera 2) was grown in serum-free medium, and the population multiplication was stimulated by the addition of 1-10 ng basic fibroblast growth factor (bFGF)/ml. The bFGF-effect was abrogated by the addition of protamine sulphate. When high concentrations of bFGF were added, a preferential effe...

journal_title：FEBS letters

authors： Schofield PN,Granerus M,Lee A,Ektröm TJ,Engström W

更新日期：1992-02-24 00:00:00