Abstract:
:The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G4-forming amylase) has been determined. The coding region for the G4-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced mature form of G4-forming amylase contains 530 amino acid residues with a calculated molecular mass of 57 740 Da. Sequence similarities between the G4-forming amylase and other amylolytic enzymes of species ranging from prokaryotes to eukaryotes are quite limited. However, three regions, which are involved in both the catalytic and substrate-binding sites of various amylolytic enzymes, are highly conserved in the G4-forming amylase of P. saccharophila.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zhou JH,Baba T,Takano T,Kobayashi S,Arai Ydoi
10.1016/0014-5793(89)81056-7subject
Has Abstractpub_date
1989-09-11 00:00:00pages
37-41issue
1eissn
0014-5793issn
1873-3468journal_volume
255pub_type
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