Abstract:
:An open reading frame encoding a putative polypeptide very similar to several lysyl-tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl-tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypeptide of the expected mass (62 kDa), thus confirming that it encodes R. meliloti lysyl-tRNA synthetase. Reverse transcription/polymerase chain reaction was used to demonstrate that this lysS gene is co-transcribed with gltX in R. meliloti. This is the first reported case of two immediately adjacent and co-transcribed genes encoding aminoacyl-tRNA synthetases.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pelchat M,Gagnon Y,Laberge S,Lapointe Jdoi
10.1016/s0014-5793(99)00385-3keywords:
subject
Has Abstractpub_date
1999-04-16 00:00:00pages
23-7issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00385-3journal_volume
449pub_type
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